FastQCFastQC Report
Thu 26 May 2016
SRR1547517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547517_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3201459
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137970.43095975928475105No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC49010.1530864521457248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTA253.8923718E-545.036
GTAATCG207.0351776E-445.01
TACGTCG207.0351776E-445.034
TTTCGCG650.044.9999961
TACGATA302.166722E-644.99999624
AGCGATC302.166722E-644.99999645
CCCGGTA600.044.99999636
CTAGACG1050.042.8571431
ACGGTCT8650.042.6589630
CGATACA900.042.50000410
CGTTTTT74600.042.4061661
TCGTCGA800.042.187532
TTCGTAA700.041.78571328
TCGCATA650.041.5384637
CCGGTTA650.041.5384645
TCACGAC9050.041.5193425
CGACGGT9050.041.5193428
CTCACGA9150.041.31147824
CTACGGG4250.041.2941173
CGTTCTA603.6379788E-1241.24999621