##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547517_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3201459
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6092013047801	31.0	31.0	33.0	30.0	34.0
2	32.04231039660355	33.0	31.0	34.0	30.0	34.0
3	32.11013353599093	33.0	31.0	34.0	30.0	34.0
4	35.83520294965514	37.0	35.0	37.0	35.0	37.0
5	35.77485015425779	37.0	35.0	37.0	35.0	37.0
6	35.83249199817958	37.0	35.0	37.0	35.0	37.0
7	36.15082966859797	37.0	35.0	37.0	35.0	37.0
8	36.15880103415349	37.0	35.0	37.0	35.0	37.0
9	38.01738020071474	39.0	39.0	39.0	35.0	39.0
10	37.46061561306892	39.0	37.0	39.0	35.0	39.0
11	37.15294995188131	39.0	37.0	39.0	33.0	39.0
12	36.50617952627224	39.0	35.0	39.0	33.0	39.0
13	36.2118062420915	39.0	35.0	39.0	32.0	39.0
14	37.19825210942886	39.0	35.0	41.0	32.0	41.0
15	37.41901458053969	39.0	35.0	41.0	32.0	41.0
16	37.55986348724129	40.0	35.0	41.0	33.0	41.0
17	37.49352935645904	39.0	35.0	41.0	33.0	41.0
18	37.396667894231975	39.0	35.0	41.0	33.0	41.0
19	37.398388047449615	39.0	35.0	41.0	33.0	41.0
20	37.272189960889705	39.0	35.0	41.0	32.0	41.0
21	37.14246723134671	39.0	35.0	41.0	32.0	41.0
22	37.07732130881576	39.0	35.0	41.0	32.0	41.0
23	37.04647162434377	39.0	35.0	41.0	32.0	41.0
24	36.924927353434796	38.0	35.0	41.0	32.0	41.0
25	36.83812817843364	38.0	35.0	41.0	32.0	41.0
26	36.78716860031629	38.0	35.0	41.0	32.0	41.0
27	36.74871706931121	38.0	35.0	41.0	31.0	41.0
28	36.76598263479245	38.0	35.0	41.0	31.0	41.0
29	36.71237395200126	38.0	35.0	41.0	31.0	41.0
30	36.69066853581445	38.0	35.0	41.0	31.0	41.0
31	36.61233643785537	38.0	35.0	41.0	31.0	41.0
32	36.478330348756614	38.0	35.0	41.0	31.0	41.0
33	36.38896203262325	38.0	35.0	41.0	30.0	41.0
34	36.28412670597999	38.0	35.0	41.0	30.0	41.0
35	36.135275822679596	38.0	35.0	41.0	30.0	41.0
36	36.00600070155514	38.0	35.0	41.0	30.0	41.0
37	35.9496042273226	38.0	35.0	41.0	30.0	41.0
38	35.81128385526724	38.0	35.0	41.0	29.0	41.0
39	35.75842545539393	38.0	35.0	41.0	29.0	41.0
40	35.5805253167384	38.0	35.0	40.0	28.0	41.0
41	35.55192523158972	38.0	35.0	40.0	28.0	41.0
42	35.520696969725364	38.0	35.0	40.0	28.0	41.0
43	35.429526975044816	38.0	34.0	40.0	28.0	41.0
44	35.3173584293911	38.0	34.0	40.0	27.0	41.0
45	35.209840575812464	37.0	34.0	40.0	27.0	41.0
46	35.18627631964051	37.0	34.0	40.0	27.0	41.0
47	35.09860691640905	37.0	34.0	40.0	27.0	41.0
48	34.97608902690929	37.0	34.0	40.0	26.0	41.0
49	34.901616731621424	37.0	34.0	40.0	26.0	41.0
50	34.78811223257896	37.0	34.0	40.0	26.0	41.0
51	34.43952585368109	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	7.0
12	5.0
13	29.0
14	47.0
15	91.0
16	210.0
17	541.0
18	1073.0
19	2150.0
20	3751.0
21	6013.0
22	9291.0
23	13928.0
24	20079.0
25	29111.0
26	39747.0
27	47903.0
28	52225.0
29	58742.0
30	69668.0
31	84251.0
32	106335.0
33	140971.0
34	254423.0
35	413867.0
36	202215.0
37	270274.0
38	445497.0
39	928727.0
40	280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.651456726448785	24.08782995502988	30.38402178506737	12.876691533453965
2	29.40237560437288	25.398232493372554	31.21195679844721	13.987435103807359
3	29.08477041249006	25.19929194782754	32.12931978825904	13.586617851423366
4	25.500748252593585	27.892251626524033	31.554269475261123	15.052730645621262
5	22.98111579751607	32.315203786773466	30.566907150770945	14.136773264939517
6	22.36177317904118	41.12031420674136	25.857179492225264	10.660733121992191
7	90.01489633320307	3.1242317955657093	5.213216848942935	1.6476550222882753
8	90.9579038806994	2.4043100348934656	5.1782015637245395	1.4595845206826012
9	86.05944976962067	5.059536917386729	6.679891886792866	2.2011214261997423
10	45.085287676649926	31.32634214587786	13.920715523765884	9.667654653706325
11	37.653832205878636	22.12185131841451	24.054001628632445	16.170314847074412
12	36.105069594831605	20.457610108391204	27.071250951519293	16.3660693452579
13	25.934144401037152	30.734362051802005	26.110626436259217	17.220867110901622
14	17.74153596844439	35.32002127779865	28.82779382775166	18.1106489260053
15	16.85981297901988	27.857361284339422	39.645392928661586	15.637432807979113
16	22.365896299156105	24.228297160763265	37.01512341716698	16.390683122913646
17	22.059473508797083	24.73997011987347	27.028208076380178	26.17234829494927
18	23.40517245418417	26.858129371639617	31.333807492146548	18.402890682029664
19	26.703918432189823	27.25082532682755	26.368196500408096	19.67705974057453
20	28.622106358382226	27.43096194578784	26.043781913183956	17.903149782645976
21	24.918669893945232	26.522313732582553	30.195357804051216	18.363658569421005
22	24.457692570793505	22.62565286639623	30.282505570116626	22.634148992693646
23	21.12458725849683	27.48115780961118	29.13946422552967	22.254790706362318
24	20.850555949646708	26.92244379828072	34.43704885803629	17.789951394036283
25	21.229164577775318	27.038328462116805	30.74582557515183	20.986681384956047
26	18.824073648920695	33.319464656583136	26.316126491077974	21.54033520341819
27	18.08194326399307	32.94560386373838	30.37493217935947	18.59752069290908
28	17.125660519157044	28.945365222543845	35.356941944282276	18.57203231401683
29	18.37009313566096	27.128912161611314	34.41668314352925	20.08431155919848
30	19.586319862287787	28.774162030499216	32.31951432143907	19.320003785773924
31	26.84772786407697	26.409021636697517	27.558778669350442	19.184471829875065
32	27.326509569543134	26.974420100335507	27.863483492994913	17.835586837126446
33	25.446429268655322	27.414438229569704	27.06322336159857	20.0759091401764
34	20.748477491043925	28.262988843524155	28.973945941522288	22.014587723909628
35	20.111549140563724	28.74954825284347	29.22723670676401	21.911665899828797
36	27.327977650190117	25.667078666320574	28.67333300223429	18.331610681255015
37	21.338708382646786	30.775811903260358	29.08926836170633	18.79621135238652
38	20.925303119608905	31.35367343451845	25.898441929132936	21.82258151673971
39	20.501746235075945	30.22022146777454	28.08282098880542	21.195211308344103
40	23.381558220798702	27.57202263093171	26.565262900446328	22.481156247823257
41	19.93116263553586	25.78302580167355	28.98025556472846	25.305555998062136
42	21.883647424502392	26.18706033717752	27.238174844656765	24.691117393663326
43	21.759703934987144	26.771856206810707	27.69096839909554	23.777471459106614
44	20.946668378386228	28.972571568150645	28.59539978491057	21.485360268552554
45	18.791369809827334	33.334551527912744	25.642027588046574	22.232051074213352
46	22.28034155677146	30.671734356117007	26.874715559374646	20.173208527736886
47	21.235942737358187	28.92856038449969	27.463634549122762	22.37186232901936
48	22.545345731430576	26.752271386264827	29.640641969801894	21.061740912502707
49	22.208936612963026	24.8999284388774	30.820697688147806	22.07043726001176
50	20.841497579697258	29.79866367178215	28.729588603196234	20.630250145324368
51	19.480805470255905	31.915604728968887	26.26730500062628	22.33628480014893
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1830.0
1	3300.0
2	4770.0
3	14773.0
4	24776.0
5	18361.5
6	11947.0
7	11376.5
8	10806.0
9	10924.5
10	11043.0
11	11163.0
12	11283.0
13	10552.0
14	9821.0
15	9237.0
16	8653.0
17	8463.0
18	8273.0
19	9962.5
20	11652.0
21	12931.5
22	14211.0
23	17178.0
24	20145.0
25	21330.0
26	32419.0
27	42323.0
28	43988.5
29	45654.0
30	56835.0
31	68016.0
32	77873.5
33	87731.0
34	96349.5
35	104968.0
36	112462.5
37	119957.0
38	138007.5
39	156058.0
40	182076.5
41	208095.0
42	238811.0
43	269527.0
44	275889.5
45	282252.0
46	269132.0
47	256012.0
48	254823.0
49	253634.0
50	236657.5
51	219681.0
52	205938.5
53	192196.0
54	178998.5
55	165801.0
56	161214.0
57	156627.0
58	142100.5
59	127574.0
60	108358.5
61	89143.0
62	77966.0
63	66789.0
64	56541.5
65	46294.0
66	37282.0
67	28270.0
68	22289.0
69	16308.0
70	13579.0
71	10850.0
72	9214.0
73	7578.0
74	6132.5
75	3402.0
76	2117.0
77	1453.0
78	789.0
79	551.0
80	313.0
81	298.0
82	283.0
83	183.0
84	83.0
85	65.5
86	48.0
87	46.0
88	44.0
89	31.0
90	18.0
91	13.0
92	8.0
93	6.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3201459.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.924063913547053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.45893092884894	12.156982915113565
2	8.773588736341884	2.618751981046856
3	2.7087951378212676	1.2127869529665027
4	1.184735161975738	0.7072425311180973
5	0.6425382313747185	0.47946408159668924
6	0.441394053239733	0.395243583696562
7	0.3010746671435899	0.31452803026405884
8	0.22035952721499594	0.26309277344924886
9	0.18547624560933054	0.24912534095265598
>10	1.611666037943423	5.340055254663399
>50	0.5855564702943261	6.465537606892625
>100	1.8432053581059358	65.10945105658983
>500	0.03868481631457648	3.4260733738539058
>1k	0.00378438420468683	0.8287576450770048
>5k	0.0	0.0
>10k+	2.1024356692704612E-4	0.4329068727190184
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13797	0.43095975928475105	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4901	0.1530864521457248	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019085048410740227	0.0
2	0.0	0.0	0.0	0.06534520666983397	0.0
3	0.0	0.0	0.0	0.09754927362805521	0.0
4	0.0	0.0	0.0	0.18001167592650727	0.0
5	0.0	0.0	0.0	0.32803793520391794	0.0
6	0.0	0.0	0.0	0.47384645563163547	0.0
7	0.0	0.0	0.0	0.5592762549824939	0.0
8	0.0	0.0	0.0	0.7478152929648638	0.0
9	0.0	0.0	0.0	0.8011659683912866	0.0
10	0.0	0.0	0.0	0.9157074946141743	0.0
11	0.0	0.0	0.0	1.081819258032041	0.0
12	0.0	0.0	0.0	1.216507848452846	0.0
13	3.123575844638335E-5	0.0	0.0	1.26604776134881	0.0
14	3.123575844638335E-5	0.0	0.0	1.2873817843676898	0.0
15	6.24715168927667E-5	0.0	0.0	1.3200231519441605	0.0
16	6.24715168927667E-5	0.0	0.0	1.3954262728337299	0.0
17	6.24715168927667E-5	0.0	0.0	1.4918822949161616	0.0
18	9.370727533915006E-5	0.0	0.0	1.6113278352151317	0.0
19	9.370727533915006E-5	0.0	0.0	1.6794530243866936	0.0
20	9.370727533915006E-5	0.0	0.0	1.7581671356715798	0.0
21	9.370727533915006E-5	0.0	0.0	1.8599332366898966	0.0
22	9.370727533915006E-5	0.0	0.0	1.9674467172623482	0.0
23	1.249430337855334E-4	0.0	0.0	2.0681820382519347	0.0
24	1.249430337855334E-4	0.0	0.0	2.1450220040300376	0.0
25	1.249430337855334E-4	0.0	0.0	2.2155523466019713	0.0
26	1.249430337855334E-4	0.0	0.0	2.2886752571249547	0.0
27	1.249430337855334E-4	0.0	0.0	2.379727492996162	0.0
28	1.249430337855334E-4	0.0	0.0	2.464282691110522	0.0
29	1.5617879223191677E-4	0.0	0.0	2.576731421517502	0.0
30	1.874145506783001E-4	0.0	0.0	2.6927410283873696	0.0
31	1.874145506783001E-4	0.0	0.0	2.7931952275509384	0.0
32	1.874145506783001E-4	0.0	0.0	2.887183624716106	0.0
33	1.874145506783001E-4	0.0	0.0	2.983577175281645	0.0
34	1.874145506783001E-4	0.0	0.0	3.095151304452126	0.0
35	1.874145506783001E-4	0.0	0.0	3.2217810691937645	0.0
36	1.874145506783001E-4	0.0	0.0	3.327982647911468	0.0
37	1.874145506783001E-4	0.0	0.0	3.4400877849755376	0.0
38	1.874145506783001E-4	0.0	0.0	3.5908003194793374	0.0
39	1.874145506783001E-4	0.0	0.0	3.9119976235834972	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGTA	25	3.8923718E-5	45.0	36
GTAATCG	20	7.0351776E-4	45.0	1
TACGTCG	20	7.0351776E-4	45.0	34
TTTCGCG	65	0.0	44.999996	1
TACGATA	30	2.166722E-6	44.999996	24
AGCGATC	30	2.166722E-6	44.999996	45
CCCGGTA	60	0.0	44.999996	36
CTAGACG	105	0.0	42.857143	1
ACGGTCT	865	0.0	42.65896	30
CGATACA	90	0.0	42.500004	10
CGTTTTT	7460	0.0	42.406166	1
TCGTCGA	80	0.0	42.1875	32
TTCGTAA	70	0.0	41.785713	28
TCGCATA	65	0.0	41.53846	37
CCGGTTA	65	0.0	41.53846	45
TCACGAC	905	0.0	41.51934	25
CGACGGT	905	0.0	41.51934	28
CTCACGA	915	0.0	41.311478	24
CTACGGG	425	0.0	41.294117	3
CGTTCTA	60	3.6379788E-12	41.249996	21
>>END_MODULE