FastQCFastQC Report
Thu 26 May 2016
SRR1547516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547516_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences5109613
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195590.3827882855316048No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGT207.035571E-445.041
CTATGCG3100.042.8225821
CGGTCTA7650.042.05882331
TCGTTAG1600.040.781251
CACGACG8300.040.39156326
CGTTTTT121700.040.156121
GCGATAT2750.040.0909089
CTATCGA900.040.00000413
TACGGGA20400.039.8161774
TACGGGT3000.039.7500044
ATTACGC1250.039.612
CGCTTAT800.039.37535
CTATTCG750.039.00000439
CGGGTAT4550.038.571436
GTACGGG15950.038.0877763
ACGGGAT23400.038.0769235
TACGCTA659.094947E-1238.0769213
TTCGGTA1300.038.0769235
GGGCGAT49300.038.0628787
ACGGGAC17500.038.0571445
Produced by FastQC (version 0.11.5)