##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547516_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	5109613
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56794242538525	31.0	31.0	33.0	30.0	34.0
2	32.0005540537023	31.0	31.0	34.0	30.0	34.0
3	32.07837658155324	33.0	31.0	34.0	30.0	34.0
4	35.79878593545147	37.0	35.0	37.0	35.0	37.0
5	35.73857804886593	37.0	35.0	37.0	33.0	37.0
6	35.788846435140975	37.0	35.0	37.0	35.0	37.0
7	36.10128653579048	37.0	35.0	37.0	35.0	37.0
8	36.09226041189421	37.0	35.0	37.0	35.0	37.0
9	37.903662371298964	39.0	38.0	39.0	35.0	39.0
10	37.41743885495829	39.0	37.0	39.0	35.0	39.0
11	37.10254455670126	39.0	37.0	39.0	33.0	39.0
12	36.62294032835755	39.0	35.0	39.0	33.0	39.0
13	36.419764667108836	39.0	35.0	39.0	32.0	39.0
14	37.53027695052443	40.0	35.0	41.0	33.0	41.0
15	37.65537076878425	40.0	35.0	41.0	33.0	41.0
16	37.70796966423876	40.0	35.0	41.0	33.0	41.0
17	37.644822024681716	40.0	35.0	41.0	33.0	41.0
18	37.51028933110981	39.0	36.0	41.0	32.0	41.0
19	37.49575554939288	39.0	36.0	41.0	32.0	41.0
20	37.37943675969198	39.0	35.0	41.0	32.0	41.0
21	37.25616049591231	39.0	35.0	41.0	32.0	41.0
22	37.19730300513953	39.0	35.0	41.0	32.0	41.0
23	37.123242601739115	39.0	35.0	41.0	32.0	41.0
24	37.01452399624003	39.0	35.0	41.0	32.0	41.0
25	36.917966585727726	39.0	35.0	41.0	31.0	41.0
26	36.891466927143014	39.0	35.0	41.0	31.0	41.0
27	36.85925822562296	39.0	35.0	41.0	31.0	41.0
28	36.838998374240866	39.0	35.0	41.0	31.0	41.0
29	36.785600201032835	39.0	35.0	41.0	31.0	41.0
30	36.744191585546695	39.0	35.0	41.0	31.0	41.0
31	36.67705205854142	39.0	35.0	41.0	31.0	41.0
32	36.565635792769434	39.0	35.0	41.0	30.0	41.0
33	36.469411088471865	39.0	35.0	41.0	30.0	41.0
34	36.356485119323125	39.0	35.0	41.0	30.0	41.0
35	36.19458342539836	39.0	35.0	41.0	30.0	41.0
36	36.09819569505557	39.0	35.0	41.0	30.0	41.0
37	36.06490902539977	39.0	35.0	41.0	30.0	41.0
38	35.933484590711664	38.0	35.0	41.0	29.0	41.0
39	35.853563273774355	38.0	35.0	41.0	29.0	41.0
40	35.69569593626758	38.0	35.0	41.0	28.0	41.0
41	35.63583817404567	38.0	35.0	40.0	28.0	41.0
42	35.604893169012996	38.0	35.0	40.0	28.0	41.0
43	35.48943608840826	38.0	35.0	40.0	27.0	41.0
44	35.36542493531311	38.0	34.0	40.0	27.0	41.0
45	35.26691121225815	38.0	34.0	40.0	27.0	41.0
46	35.207668760823964	38.0	34.0	40.0	26.0	41.0
47	35.13560635609781	38.0	34.0	40.0	26.0	41.0
48	35.01662063252149	37.0	34.0	40.0	26.0	41.0
49	34.904155950754	37.0	34.0	40.0	26.0	41.0
50	34.81226484275815	37.0	34.0	40.0	26.0	41.0
51	34.451770809256985	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	14.0
11	43.0
12	49.0
13	65.0
14	82.0
15	195.0
16	455.0
17	946.0
18	2211.0
19	4235.0
20	7118.0
21	10898.0
22	16705.0
23	23707.0
24	33560.0
25	46983.0
26	63344.0
27	75556.0
28	84083.0
29	93934.0
30	110058.0
31	134765.0
32	167453.0
33	217740.0
34	369017.0
35	554577.0
36	349750.0
37	471625.0
38	752209.0
39	1517802.0
40	429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.43607431717432	23.334330799612417	30.194987369884963	13.034607513328309
2	30.528535135635515	25.326634326317865	30.409074033591192	13.735756504455427
3	30.434105283511688	25.11426990654674	30.296854184455846	14.154770625485726
4	26.39327087981027	28.022122223346464	30.157137145220197	15.427469751623068
5	24.584816893177624	31.63752323316854	28.96313282434501	14.814527049308824
6	23.108403708852315	40.17973572558235	25.973493491581458	10.738367073983882
7	87.89558035021439	3.8832490836390154	6.153636293003012	2.0675342731435826
8	88.02020818406405	3.698401424922005	6.168334079312856	2.113056311701101
9	82.20499282430978	6.771041172785493	7.655922278262562	3.3680437246421597
10	41.93051411134268	34.4469727942214	13.302514300006674	10.319998794429246
11	33.64988698752723	23.25340490561614	26.289505682720005	16.807202424136623
12	32.31131203087201	22.0193779842035	27.703467953443834	17.965842031480662
13	25.396072070428815	28.36208926194606	27.348587848042506	18.89325081958262
14	19.204996542791008	31.798298618701654	29.152051241454096	19.84465359705324
15	18.405483937824645	28.247697036938025	36.23252876489863	17.1142902603387
16	23.024757452276717	26.21182856705586	33.71809176154828	17.04532221911914
17	23.504656810603855	24.74093439170442	27.532241678577225	24.222167119114502
18	23.877405196831933	26.8952854159405	30.01156839079594	19.215740996431627
19	27.58030794112979	26.728423463773087	26.630216417564306	19.06105217753282
20	28.278325579647618	26.737015112494824	27.31024834953254	17.674410958325023
21	26.217543285567814	25.473553476554876	30.03753904649922	18.271364191378094
22	24.976294682200002	23.104058957106925	29.676043958710768	22.2436024019823
23	22.332317535594182	27.128786465824316	29.232566145420407	21.306329853161092
24	21.950742649198677	25.814244640445374	32.58984584546814	19.645166864887813
25	21.694147873821365	27.05050265059213	29.75381892914395	21.501530546442556
26	20.99157020306626	31.226102642215757	26.987856027452565	20.794471127265414
27	19.402056476684244	31.05428923873491	30.08991091888955	19.453743365691295
28	18.538253288458442	28.759477479018468	32.79005670292447	19.912212529598623
29	19.44879191437786	27.207187706779358	33.119572852190565	20.224447526652213
30	21.48376012038485	27.183369855994965	31.201619379001894	20.13125064461829
31	25.25539605445657	26.631879948637987	27.654110007939934	20.458613988965503
32	25.928128020654402	27.257915619049815	27.920098841145112	18.893857519150668
33	24.285146448468797	27.331326266783805	28.04883657529445	20.334690709452946
34	21.13332262149795	27.628941761342784	29.682071812483645	21.555663804675618
35	20.76628895378182	27.444955224593333	30.002096049152843	21.786659772472007
36	24.507785618989146	27.25601723653044	28.292631946881304	19.943565197599114
37	21.48203787644974	29.71590999161776	28.616785654803994	20.1852664771285
38	21.654614547129107	30.408173769716022	26.65462139696294	21.28259028619193
39	21.388919278231054	29.179470147739174	28.229280769404653	21.202329804625126
40	23.538436276876546	27.35220847449699	27.572675269144646	21.536679979481814
41	20.262708741346948	26.416384176257573	28.941957052324707	24.37895003007077
42	22.3175218945153	26.22744618819468	27.51382541104385	23.941206506246168
43	22.05313787952238	27.187107908172305	27.348646560903926	23.411107651401387
44	20.541340410712124	28.85482716597128	28.41639865876339	22.187433764553205
45	20.47147210561739	30.130794641394566	27.07465320759126	22.323080045396786
46	22.02530798320734	29.529555369457533	26.834517604366514	21.610619042968615
47	21.286817612214467	28.593652787402878	28.252139643452452	21.867389956930204
48	22.489570149441846	27.036587702434606	29.436691193638342	21.037150954485202
49	21.899134044006853	26.535629997810013	29.80523182479769	21.760004133385443
50	20.59388842168673	29.286210129808264	28.64166033709402	21.47824111141098
51	20.568093904567725	29.240003107867462	27.47153257986466	22.720370407700152
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3062.0
1	5947.5
2	8833.0
3	22901.5
4	36970.0
5	27577.0
6	18184.0
7	17356.5
8	16529.0
9	17040.0
10	17551.0
11	17547.0
12	17543.0
13	17735.0
14	17927.0
15	17105.0
16	16283.0
17	16590.5
18	16898.0
19	16831.0
20	16764.0
21	20380.5
22	23997.0
23	26237.0
24	28477.0
25	34819.0
26	48473.5
27	55786.0
28	66218.0
29	76650.0
30	96216.0
31	115782.0
32	132085.0
33	148388.0
34	169619.0
35	190850.0
36	192233.5
37	193617.0
38	212483.5
39	231350.0
40	271153.5
41	310957.0
42	351131.5
43	391306.0
44	405236.5
45	419167.0
46	405511.0
47	391855.0
48	381474.5
49	371094.0
50	356596.5
51	342099.0
52	328620.5
53	315142.0
54	287297.5
55	259453.0
56	248227.0
57	237001.0
58	225341.5
59	213682.0
60	200479.5
61	187277.0
62	159108.0
63	130939.0
64	114267.5
65	97596.0
66	76563.5
67	55531.0
68	46717.5
69	37904.0
70	31484.0
71	25064.0
72	19323.0
73	13582.0
74	10341.5
75	6196.5
76	5292.0
77	3783.0
78	2274.0
79	1816.0
80	1358.0
81	1067.5
82	777.0
83	521.5
84	266.0
85	203.0
86	140.0
87	99.0
88	58.0
89	51.0
90	44.0
91	34.5
92	25.0
93	17.5
94	10.0
95	11.0
96	12.0
97	8.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5109613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.196934720091177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.88006540016096	12.443260087630424
2	8.596584718310684	2.6128347355980175
3	2.657755013999785	1.2116918834924926
4	1.1476012500531507	0.6976008512701105
5	0.6870392495268359	0.5220445312599878
6	0.4083508950058903	0.3723409136577193
7	0.2785200785973331	0.2962856016874834
8	0.2066431834489538	0.2512274375380461
9	0.16680742594925	0.22814654066794757
>10	1.469595054432104	5.093227993092897
>50	0.6278378179654097	7.083198061776005
>100	1.8393193492436324	65.35220117253675
>500	0.030375677446793356	2.710777886746227
>1k	0.0033750752718659286	0.7393174070982246
>5k	0.0	0.0
>10k+	1.2981058737945877E-4	0.3858448959477593
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19559	0.3827882855316048	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.871286142414308E-5	0.0	0.0	0.019825376207552314	0.0
2	5.871286142414308E-5	0.0	0.0	0.06624767864024145	0.0
3	5.871286142414308E-5	0.0	0.0	0.09677836658079585	0.0
4	5.871286142414308E-5	0.0	0.0	0.16001211833459794	0.0
5	5.871286142414308E-5	0.0	0.0	0.29419057764257295	0.0
6	5.871286142414308E-5	0.0	0.0	0.414043098763057	0.0
7	5.871286142414308E-5	0.0	0.0	0.4888432842174153	0.0
8	7.828381523219078E-5	0.0	0.0	0.6342946129188257	0.0
9	7.828381523219078E-5	0.0	0.0	0.683613416515106	0.0
10	9.785476904023846E-5	0.0	0.0	0.7782976910384407	0.0
11	9.785476904023846E-5	0.0	0.0	0.9326538037225128	0.0
12	9.785476904023846E-5	0.0	0.0	1.0507840809078888	0.0
13	9.785476904023846E-5	0.0	0.0	1.1001811683194012	0.0
14	9.785476904023846E-5	0.0	0.0	1.121826643231102	0.0
15	9.785476904023846E-5	0.0	0.0	1.1586004654364235	0.0
16	1.1742572284828616E-4	0.0	0.0	1.236747283991958	0.0
17	1.1742572284828616E-4	0.0	0.0	1.3354240330921343	0.0
18	1.1742572284828616E-4	0.0	0.0	1.4573706462700795	0.0
19	1.1742572284828616E-4	0.0	0.0	1.5303311620664815	0.0
20	1.1742572284828616E-4	0.0	0.0	1.6092216768667216	0.0
21	1.1742572284828616E-4	0.0	0.0	1.709131396056805	0.0
22	1.1742572284828616E-4	0.0	0.0	1.815362533326888	0.0
23	1.1742572284828616E-4	0.0	0.0	1.9267799733561035	0.0
24	1.1742572284828616E-4	1.9570953808047694E-5	0.0	2.010993787592133	0.0
25	1.1742572284828616E-4	1.9570953808047694E-5	0.0	2.089140606147667	0.0
26	1.1742572284828616E-4	1.9570953808047694E-5	0.0	2.1661131674747187	0.0
27	1.3699667665633384E-4	1.9570953808047694E-5	0.0	2.2481154639304384	0.0
28	1.3699667665633384E-4	1.9570953808047694E-5	0.0	2.3305287504161276	0.0
29	1.3699667665633384E-4	1.9570953808047694E-5	0.0	2.4279921003802047	0.0
30	1.3699667665633384E-4	1.9570953808047694E-5	0.0	2.5394291113632286	0.0
31	1.3699667665633384E-4	1.9570953808047694E-5	0.0	2.640943648765572	0.0
32	1.5656763046438155E-4	1.9570953808047694E-5	0.0	2.7396986816809807	0.0
33	1.5656763046438155E-4	1.9570953808047694E-5	0.0	2.836868467337937	0.0
34	1.5656763046438155E-4	1.9570953808047694E-5	0.0	2.946720231062509	0.0
35	1.5656763046438155E-4	1.9570953808047694E-5	0.0	3.06770786750386	0.0
36	1.5656763046438155E-4	1.9570953808047694E-5	0.0	3.17928187516354	0.0
37	1.5656763046438155E-4	1.9570953808047694E-5	0.0	3.2961204693975845	0.0
38	1.5656763046438155E-4	1.9570953808047694E-5	0.0	3.4254257612073555	0.0
39	1.5656763046438155E-4	1.9570953808047694E-5	0.0	3.6112715385685767	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATCGT	20	7.035571E-4	45.0	41
CTATGCG	310	0.0	42.822582	1
CGGTCTA	765	0.0	42.058823	31
TCGTTAG	160	0.0	40.78125	1
CACGACG	830	0.0	40.391563	26
CGTTTTT	12170	0.0	40.15612	1
GCGATAT	275	0.0	40.090908	9
CTATCGA	90	0.0	40.000004	13
TACGGGA	2040	0.0	39.816177	4
TACGGGT	300	0.0	39.750004	4
ATTACGC	125	0.0	39.6	12
CGCTTAT	80	0.0	39.375	35
CTATTCG	75	0.0	39.000004	39
CGGGTAT	455	0.0	38.57143	6
GTACGGG	1595	0.0	38.087776	3
ACGGGAT	2340	0.0	38.076923	5
TACGCTA	65	9.094947E-12	38.07692	13
TTCGGTA	130	0.0	38.07692	35
GGGCGAT	4930	0.0	38.062878	7
ACGGGAC	1750	0.0	38.057144	5
>>END_MODULE