FastQCFastQC Report
Thu 26 May 2016
SRR1547515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547515_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1670980
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88230.5280135010592587No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36500.21843469101964114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16800.10053980298986225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCA750.045.00000413
ATAGCGC750.045.00000412
ATCGTAG750.045.0000041
CGTCGGG750.045.0000043
TATCACG406.8175723E-945.012
AACGTAG253.8915685E-545.01
AACGTAC253.8915685E-545.010
CTATGCG406.8175723E-945.01
AGCCGAC502.1827873E-1145.026
GTACCGG207.0342066E-445.02
TCTTGCG207.0342066E-445.01
CGAACTA351.2123746E-745.038
TCGCACA351.2123746E-745.036
TACCCCG253.8915685E-545.024
ACTGCGC351.2123746E-745.034
CACGGTT207.0342066E-445.013
GATCGCG453.8562575E-1045.01
GTCGAAA207.0342066E-445.012
CGTGCTC207.0342066E-445.026
CGACGGC207.0342066E-445.034
Produced by FastQC (version 0.11.5)