##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547515_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1670980
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.595081927970412	31.0	31.0	33.0	30.0	34.0
2	32.0143664197058	31.0	31.0	34.0	30.0	34.0
3	32.04204718189326	33.0	31.0	34.0	30.0	34.0
4	35.77096793498426	37.0	35.0	37.0	35.0	37.0
5	35.71504685872961	37.0	35.0	37.0	33.0	37.0
6	35.76527726244479	37.0	35.0	37.0	35.0	37.0
7	36.122054722378486	37.0	35.0	37.0	35.0	37.0
8	36.143425415025916	37.0	35.0	37.0	35.0	37.0
9	38.0000365055237	39.0	39.0	39.0	35.0	39.0
10	37.47388957378305	39.0	37.0	39.0	35.0	39.0
11	37.08656117966702	39.0	37.0	39.0	33.0	39.0
12	36.151681647895245	37.0	35.0	39.0	32.0	39.0
13	35.71313301176555	37.0	35.0	39.0	31.0	39.0
14	36.72806855857042	38.0	35.0	41.0	31.0	41.0
15	37.007795425438964	38.0	35.0	41.0	32.0	41.0
16	37.155866018743495	38.0	35.0	41.0	33.0	41.0
17	37.086401393194414	38.0	35.0	41.0	32.0	41.0
18	37.00230882476152	38.0	35.0	41.0	32.0	41.0
19	36.971753102969515	38.0	35.0	41.0	32.0	41.0
20	36.84504542244671	38.0	35.0	41.0	32.0	41.0
21	36.683537205711616	38.0	35.0	41.0	32.0	41.0
22	36.565016337717985	38.0	35.0	41.0	31.0	41.0
23	36.57953835473794	38.0	35.0	40.0	32.0	41.0
24	36.42011095285402	38.0	35.0	40.0	31.0	41.0
25	36.31824917114508	37.0	35.0	40.0	31.0	41.0
26	36.254640390668946	37.0	35.0	40.0	31.0	41.0
27	36.2319806341189	37.0	35.0	40.0	31.0	41.0
28	36.27077283989036	37.0	35.0	40.0	31.0	41.0
29	36.258041987336775	37.0	35.0	40.0	31.0	41.0
30	36.24259057559037	37.0	35.0	40.0	31.0	41.0
31	36.11715400543394	37.0	35.0	40.0	31.0	41.0
32	35.94108128164311	37.0	35.0	40.0	30.0	41.0
33	35.837558797831214	37.0	35.0	40.0	30.0	41.0
34	35.73971082837616	37.0	35.0	40.0	30.0	41.0
35	35.572775257633246	37.0	35.0	40.0	30.0	41.0
36	35.417718943374545	37.0	35.0	40.0	29.0	41.0
37	35.38064848172928	37.0	34.0	40.0	29.0	41.0
38	35.2965493303331	37.0	34.0	40.0	29.0	41.0
39	35.28899508073107	37.0	34.0	40.0	28.0	41.0
40	35.11255490789836	37.0	34.0	40.0	27.0	41.0
41	35.07907216124669	36.0	34.0	40.0	27.0	41.0
42	35.06928748399143	36.0	34.0	40.0	27.0	41.0
43	34.96113717698596	36.0	34.0	40.0	27.0	41.0
44	34.804978515601626	36.0	34.0	40.0	26.0	41.0
45	34.698356652982085	36.0	34.0	40.0	26.0	41.0
46	34.668329962058195	36.0	34.0	40.0	26.0	41.0
47	34.600254940214725	35.0	34.0	40.0	26.0	41.0
48	34.51194867682438	35.0	34.0	40.0	26.0	41.0
49	34.46759446552323	36.0	34.0	40.0	25.0	41.0
50	34.347125040395454	35.0	34.0	40.0	24.0	41.0
51	33.956415396952686	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	1.0
12	3.0
13	6.0
14	28.0
15	61.0
16	148.0
17	342.0
18	768.0
19	1396.0
20	2407.0
21	3793.0
22	5799.0
23	8404.0
24	12260.0
25	17632.0
26	23753.0
27	27572.0
28	30129.0
29	33854.0
30	39186.0
31	47528.0
32	59491.0
33	79393.0
34	151670.0
35	277186.0
36	98232.0
37	124335.0
38	203473.0
39	421984.0
40	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.684879531771777	24.466420902703803	29.79987791595351	14.048821649570911
2	28.27179260074926	27.063040850279478	30.858179032663468	13.806987516307794
3	28.03648158565632	26.660402877353413	31.651007193383524	13.652108343606745
4	25.485583310392702	27.582137428335468	32.02581718512489	14.906462076146932
5	24.557445331482125	32.244072340782054	29.020455062298772	14.178027265437048
6	21.595351231014135	40.36649151994638	26.937306251421322	11.100850997618164
7	88.61093490047757	3.9077666997809666	6.10869070844654	1.3726076912949288
8	90.14638116554357	2.5207961794874865	5.84327759757747	1.4895450573914708
9	85.54297478126608	5.376844725849502	6.775724425187614	2.3044560676968007
10	50.7262803863601	30.028007516547177	11.071048127446169	8.174663969646554
11	45.27510802044309	19.206094627105053	22.344432608409438	13.17436474404242
12	42.445929933332536	20.38061496846162	23.145579240924487	14.027875857281355
13	23.324456307077284	37.5107421991885	22.89422973344983	16.270571760284383
14	14.365103113143185	42.696621144478094	26.824737579145175	16.11353816323355
15	14.33799327340842	26.15931968066644	46.45998156770278	13.042705478222361
16	17.90823349172342	21.463273049348285	44.96505044943685	15.663443009491438
17	17.500209457922896	21.35333756238854	29.189457683515062	31.956995296173503
18	21.40492405654167	25.320949382996805	33.12690756322637	20.147218997235157
19	28.0079354630217	26.632574896168716	27.32306790027409	18.036421740535495
20	31.91432572502364	25.46248309375337	25.68307220912279	16.940118972100205
21	24.370010413051023	27.135453446480508	29.071263569881147	19.42327257058732
22	22.231504865408322	24.8542172856647	27.58201773809381	25.332260110833165
23	19.556547654669714	29.198613987001636	26.96034662293983	24.284491735388812
24	20.88887957964787	25.033812493267426	36.72910507606315	17.348202851021558
25	17.659577014685993	26.747597218398784	34.97396737244012	20.618858394475097
26	17.145208201175357	35.64082155381872	28.242288956181405	18.97168128882452
27	17.887108164071382	35.62681779554513	29.928305545248897	16.557768495134592
28	15.83376222336593	29.8958694897605	38.14887072256999	16.12149756430358
29	15.58696094507415	26.40983135644951	38.30261283797532	19.700594860501024
30	18.04671510131779	30.569605860034233	32.33467785371459	19.049001184933392
31	27.957725406647594	28.001172964368216	25.971406001268715	18.069695627715472
32	28.92978970424541	28.85252965325737	26.361775724425186	15.855904918072032
33	26.9802152030545	29.57839112377168	25.00532621575363	18.436067457420197
34	20.716884702390214	29.286227243892803	27.73079270847048	22.2660953452465
35	19.585572538270952	28.169756669738717	29.5725263019306	22.672144490059726
36	27.621216292235694	26.287986690445127	28.22547247722893	17.865324540090246
37	20.558055751714562	31.126345019090596	29.601311805048535	18.71428742414631
38	19.119438892147123	33.099438652766636	24.606219104956374	23.174903350129863
39	19.831476139750325	31.393553483584487	28.614226382123064	20.160743994542123
40	23.264431650887506	27.26860884032125	26.625752552394406	22.841206956396846
41	17.385665896659443	25.765119869777013	29.81166740475649	27.037546828807045
42	21.676201989251815	25.98918000215442	27.305652970113346	25.02896503848041
43	23.682449819866186	26.164645896420062	25.75817783576105	24.394726447952696
44	19.629857927683155	31.980933344504425	27.289016026523356	21.100192701289064
45	18.22188176997929	36.64418484960921	23.464912805658955	21.66902057475254
46	22.024739972950005	33.45138780835198	25.13183880118254	19.39203341751547
47	21.6568720152246	29.03182563525596	26.47990999293828	22.83139235658117
48	23.25036804749309	25.527055979126022	30.19102562568074	21.03155034770015
49	20.273312666818274	25.243449951525456	31.29397120252786	23.189266179128413
50	18.73858454320219	32.27788483404948	27.368071431136222	21.615459191612107
51	17.532166752444674	34.506217908053955	24.85463620151049	23.10697913799088
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2118.0
1	2541.0
2	2964.0
3	8961.5
4	14959.0
5	11139.5
6	7320.0
7	7283.0
8	7246.0
9	7510.5
10	7775.0
11	7804.5
12	7834.0
13	7457.5
14	7081.0
15	6596.5
16	6112.0
17	5607.0
18	5102.0
19	4889.0
20	4676.0
21	5492.0
22	6308.0
23	8374.5
24	10441.0
25	12073.0
26	15652.5
27	17600.0
28	18702.0
29	19804.0
30	26211.0
31	32618.0
32	36854.0
33	41090.0
34	45746.5
35	50403.0
36	51349.0
37	52295.0
38	64515.5
39	76736.0
40	100768.5
41	124801.0
42	144536.0
43	164271.0
44	168716.5
45	173162.0
46	166454.5
47	159747.0
48	142776.0
49	125805.0
50	116823.0
51	107841.0
52	99348.5
53	90856.0
54	84766.0
55	78676.0
56	70645.0
57	62614.0
58	59426.5
59	56239.0
60	54404.0
61	52569.0
62	44336.0
63	36103.0
64	27948.5
65	19794.0
66	15802.5
67	11811.0
68	8467.5
69	5124.0
70	4229.5
71	3335.0
72	2570.0
73	1805.0
74	1420.0
75	786.5
76	538.0
77	350.5
78	163.0
79	154.0
80	145.0
81	186.5
82	228.0
83	135.0
84	42.0
85	38.5
86	35.0
87	30.0
88	25.0
89	19.0
90	13.0
91	14.5
92	16.0
93	8.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1670980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.84904259222488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.17040501752702	13.023222489431546
2	8.54495940328843	2.708588510631017
3	2.5463577561749475	1.2107199759797675
4	1.0885159948469965	0.6900774545859234
5	0.6532002948900959	0.517629964748349
6	0.41263220998721883	0.3923895282606787
7	0.30422255456726693	0.33751453573964496
8	0.21240816111843877	0.269317279400184
9	0.15578690067040932	0.22221659016324213
>10	1.3867996527928854	4.96629640477176
>50	0.6302294444287135	7.420800713726039
>100	1.8766495068323075	65.69766849634898
>500	0.01593596427152384	1.5700819748365666
>1k	0.0015177108830022703	0.44289914376924866
>5k	3.7942772075056757E-4	0.5305769376070715
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8823	0.5280135010592587	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3650	0.21843469101964114	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1680	0.10053980298986225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.196902416545979E-4	0.0	0.0	0.03530862128810638	0.0
2	1.196902416545979E-4	0.0	0.0	0.13812253886940598	0.0
3	1.196902416545979E-4	0.0	0.0	0.19557385486361298	0.0
4	1.196902416545979E-4	0.0	0.0	0.3578139774264204	0.0
5	1.196902416545979E-4	0.0	0.0	0.6259201187327197	0.0
6	1.7953536248189686E-4	0.0	0.0	0.8721229458162276	0.0
7	1.7953536248189686E-4	0.0	0.0	1.0098265688398425	0.0
8	1.7953536248189686E-4	0.0	0.0	1.2918167781780752	0.0
9	1.7953536248189686E-4	0.0	0.0	1.3621347951501515	0.0
10	1.7953536248189686E-4	0.0	0.0	1.5507067708769704	0.0
11	1.7953536248189686E-4	0.0	0.0	1.762618343726436	0.0
12	1.7953536248189686E-4	0.0	0.0	1.953584124286347	0.0
13	1.7953536248189686E-4	0.0	0.0	2.019832673042167	0.0
14	1.7953536248189686E-4	0.0	0.0	2.0463440615686603	0.0
15	1.7953536248189686E-4	0.0	0.0	2.0955367508887	0.0
16	1.7953536248189686E-4	0.0	0.0	2.1949394965828435	0.0
17	1.7953536248189686E-4	0.0	0.0	2.321930842978372	0.0
18	1.7953536248189686E-4	0.0	0.0	2.485308022836898	0.0
19	1.7953536248189686E-4	0.0	0.0	2.5693904175992532	0.0
20	1.7953536248189686E-4	0.0	0.0	2.647248919795569	0.0
21	1.7953536248189686E-4	0.0	0.0	2.761792481059019	0.0
22	1.7953536248189686E-4	0.0	0.0	2.8855521909298734	0.0
23	1.7953536248189686E-4	0.0	0.0	3.0248716322158256	0.0
24	1.7953536248189686E-4	0.0	0.0	3.118110330464757	0.0
25	1.7953536248189686E-4	0.0	0.0	3.201295048414703	0.0
26	1.7953536248189686E-4	0.0	0.0	3.2810685944774924	0.0
27	1.7953536248189686E-4	0.0	0.0	3.3568325174448526	0.0
28	1.7953536248189686E-4	0.0	0.0	3.4433087170402996	0.0
29	1.7953536248189686E-4	0.0	0.0	3.532717327556284	0.0
30	1.7953536248189686E-4	0.0	0.0	3.663957677530551	0.0
31	2.393804833091958E-4	0.0	0.0	3.789452895905397	0.0
32	2.393804833091958E-4	0.0	0.0	3.892326658607524	0.0
33	2.393804833091958E-4	0.0	0.0	3.9990305090425977	0.0
34	2.393804833091958E-4	0.0	0.0	4.106153275323463	0.0
35	2.393804833091958E-4	0.0	0.0	4.241522938634813	0.0
36	2.393804833091958E-4	0.0	0.0	4.356545260864881	0.0
37	2.393804833091958E-4	0.0	0.0	4.476235502519479	0.0
38	2.393804833091958E-4	0.0	0.0	4.596703730744832	0.0
39	2.393804833091958E-4	0.0	0.0	4.723395851536225	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGCA	75	0.0	45.000004	13
ATAGCGC	75	0.0	45.000004	12
ATCGTAG	75	0.0	45.000004	1
CGTCGGG	75	0.0	45.000004	3
TATCACG	40	6.8175723E-9	45.0	12
AACGTAG	25	3.8915685E-5	45.0	1
AACGTAC	25	3.8915685E-5	45.0	10
CTATGCG	40	6.8175723E-9	45.0	1
AGCCGAC	50	2.1827873E-11	45.0	26
GTACCGG	20	7.0342066E-4	45.0	2
TCTTGCG	20	7.0342066E-4	45.0	1
CGAACTA	35	1.2123746E-7	45.0	38
TCGCACA	35	1.2123746E-7	45.0	36
TACCCCG	25	3.8915685E-5	45.0	24
ACTGCGC	35	1.2123746E-7	45.0	34
CACGGTT	20	7.0342066E-4	45.0	13
GATCGCG	45	3.8562575E-10	45.0	1
GTCGAAA	20	7.0342066E-4	45.0	12
CGTGCTC	20	7.0342066E-4	45.0	26
CGACGGC	20	7.0342066E-4	45.0	34
>>END_MODULE