FastQCFastQC Report
Thu 26 May 2016
SRR1547514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547514_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2401709
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126380.5262086289388098No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44020.18328615165284387No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG38780.16146835440929772No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG35450.14760322753505942No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG26100.10867261604132723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG302.1664928E-645.0000041
ATTACGC302.1664928E-645.00000441
GCGCAAC600.045.00000427
CCGTCGA302.1664928E-645.00000424
ATATCGT302.1664928E-645.00000411
CGAGCAA302.1664928E-645.00000433
CACCGTA453.8562575E-1045.00000425
TATCGGT302.1664928E-645.00000428
ATACCCG302.1664928E-645.00000422
ACGACTA453.8562575E-1045.00000410
TACGTGC302.1664928E-645.0000041
TACCGTA302.1664928E-645.00000445
CTTCGTA406.8212103E-945.012
AAACTCG253.89208E-545.039
GGTACGT207.0348236E-445.09
CGTATTA502.1827873E-1145.013
CCCTCGA253.89208E-545.023
CGTATCA253.89208E-545.011
CACGGTA207.0348236E-445.018
GATCGAC800.045.09