##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547514_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2401709
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63148782804245	31.0	31.0	33.0	30.0	34.0
2	32.0622619143285	33.0	31.0	34.0	30.0	34.0
3	32.09211440686611	33.0	31.0	34.0	30.0	34.0
4	35.81896349641026	37.0	35.0	37.0	35.0	37.0
5	35.76033024816912	37.0	35.0	37.0	35.0	37.0
6	35.81514579826282	37.0	35.0	37.0	35.0	37.0
7	36.15453329275112	37.0	35.0	37.0	35.0	37.0
8	36.16241143285885	37.0	35.0	37.0	35.0	37.0
9	37.99705251552124	39.0	39.0	39.0	35.0	39.0
10	37.508877220346015	39.0	37.0	39.0	35.0	39.0
11	37.17463148116612	39.0	37.0	39.0	34.0	39.0
12	36.449924616179565	38.0	35.0	39.0	33.0	39.0
13	36.119334607148495	38.0	35.0	39.0	32.0	39.0
14	37.198754720076415	39.0	35.0	41.0	32.0	41.0
15	37.3839299432196	39.0	35.0	41.0	33.0	41.0
16	37.51009510311199	39.0	35.0	41.0	33.0	41.0
17	37.43808721206441	39.0	35.0	41.0	33.0	41.0
18	37.34454965193535	39.0	35.0	41.0	33.0	41.0
19	37.29920194328289	39.0	35.0	41.0	32.0	41.0
20	37.170298316740286	39.0	35.0	41.0	32.0	41.0
21	37.03470570331377	39.0	35.0	41.0	32.0	41.0
22	36.93673421717619	38.0	35.0	41.0	32.0	41.0
23	36.87980475569688	38.0	35.0	41.0	32.0	41.0
24	36.77478495521314	38.0	35.0	41.0	32.0	41.0
25	36.68166917807278	38.0	35.0	41.0	32.0	41.0
26	36.61249968251774	38.0	35.0	41.0	31.0	41.0
27	36.58949189931003	38.0	35.0	41.0	31.0	41.0
28	36.59173530182049	38.0	35.0	41.0	31.0	41.0
29	36.59345366153851	38.0	35.0	41.0	31.0	41.0
30	36.5621384605712	38.0	35.0	40.0	31.0	41.0
31	36.49486719665038	38.0	35.0	41.0	31.0	41.0
32	36.345880371019135	38.0	35.0	41.0	31.0	41.0
33	36.23484402148637	38.0	35.0	41.0	30.0	41.0
34	36.12688881125898	38.0	35.0	41.0	30.0	41.0
35	35.96650260293816	38.0	35.0	41.0	30.0	41.0
36	35.840633898611365	38.0	35.0	41.0	30.0	41.0
37	35.75217230730284	38.0	35.0	41.0	29.0	41.0
38	35.660329790161924	38.0	35.0	41.0	29.0	41.0
39	35.624314602643366	38.0	35.0	40.0	29.0	41.0
40	35.446492476815465	38.0	35.0	40.0	28.0	41.0
41	35.42625855172296	38.0	35.0	40.0	28.0	41.0
42	35.40604586150945	38.0	35.0	40.0	28.0	41.0
43	35.30349888350337	37.0	34.0	40.0	27.0	41.0
44	35.16541471094125	37.0	34.0	40.0	27.0	41.0
45	35.07726581363521	37.0	34.0	40.0	27.0	41.0
46	35.02093883980116	37.0	34.0	40.0	27.0	41.0
47	34.95301512381392	37.0	34.0	40.0	27.0	41.0
48	34.83991482731671	37.0	34.0	40.0	26.0	41.0
49	34.788244121165384	36.0	34.0	40.0	26.0	41.0
50	34.67859886439198	36.0	34.0	40.0	26.0	41.0
51	34.29319788533915	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	14.0
12	9.0
13	17.0
14	24.0
15	47.0
16	161.0
17	374.0
18	887.0
19	1822.0
20	2951.0
21	4752.0
22	7210.0
23	10525.0
24	15728.0
25	22591.0
26	31109.0
27	37301.0
28	40954.0
29	45209.0
30	53109.0
31	63951.0
32	80477.0
33	105757.0
34	196825.0
35	344391.0
36	147977.0
37	192453.0
38	312870.0
39	681904.0
40	306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.197447734092684	23.860342780911424	30.04635449173901	13.895854993256885
2	29.490292121152063	26.701486316618706	30.020997547996032	13.787224014233198
3	29.233724818452195	25.114199930133086	32.20531713042671	13.446758120988015
4	27.248638365430615	26.60330622902275	31.361043323733224	14.787012081813408
5	23.999035686671448	32.82858164748519	29.108730491495848	14.063652174347515
6	22.460880981001445	41.54970481436344	25.813618552455775	10.175795652179344
7	88.40267492856128	3.9695899877961907	6.0189639960544765	1.6087710875880468
8	89.0974718419259	3.430682068477072	5.696193835306442	1.7756522542905904
9	83.81531651003515	7.039903668595987	6.9498844364575385	2.1948953849113275
10	45.415576991217506	35.36477566599451	11.298787654957366	7.920859687830624
11	41.57181407073047	20.497903784346896	23.214094630115472	14.716187514807164
12	39.64177175502944	20.115176318196752	25.354612069988498	14.888439856785315
13	24.000950989482906	34.247446297615575	25.14967466916267	16.60192804373885
14	16.66962983442207	38.56853598833164	27.54451101278298	17.217323164463306
15	15.749077011411458	28.7845030351304	41.7285774421464	13.737842511311737
16	19.2841430831129	22.468625466282553	42.44898112135983	15.798250329244718
17	19.301339171398364	22.92188604031546	28.605214037171027	29.171560751115145
18	21.24516333993835	27.69182278119456	31.662495331449396	19.400518547417693
19	27.84796159734589	26.69748916292523	26.389458506421885	19.06509073330699
20	31.269650070012645	25.76998295796868	26.115153834207227	16.84521313781145
21	24.687920143531127	26.754032232880835	29.295347604559918	19.262700019028117
22	24.63312582831642	24.945486734654367	27.44516508869309	22.976222348336123
23	21.04763732825251	28.809776704838097	28.249967002663517	21.892618964245877
24	21.7593388707791	25.896601128613	34.69966594620747	17.64439405440043
25	19.931015789173458	27.570617422843487	31.55307324909054	20.945293538892514
26	18.27261337655811	35.57991413614222	26.4027823520668	19.74469013523287
27	19.90170332875465	34.49289651660546	28.315753490535283	17.289646664104602
28	15.941939677121583	31.058550390576045	35.79934121910689	17.20016871319548
29	17.690694418016502	27.753820300461047	34.71586274606957	19.83962253545288
30	19.054098560649937	30.148864829169565	31.7099615315594	19.087075078621098
31	27.189888533540074	27.922533495939767	25.967300784566323	18.920277185953836
32	27.311593536102833	29.080500593535685	27.023715196137417	16.584190674224065
33	25.521909606867442	30.508275565441107	25.945108254164012	18.024706573527435
34	20.495530474341397	29.945467997996428	28.138005062228604	21.42099646543357
35	20.310120834788894	27.823978675185046	29.997764092152714	21.868136397873347
36	27.575572227942686	26.998441526429723	27.144420910276807	18.281565335350784
37	21.36641033530707	31.91423274010298	29.13737675963241	17.581980164957535
38	20.276644672606047	32.20635805586772	25.135434809129663	22.381562462396566
39	21.292462991977796	30.177261275200284	28.380457415948396	20.149818316873528
40	22.89969350991315	28.13138477642379	27.137384254295586	21.831537459367475
41	18.144412999243457	27.244308115596024	29.35976007084955	25.251518814310973
42	21.86347305189763	28.480302984249967	26.150628573236805	23.5055953906156
43	23.656363031491328	26.340743195782668	26.91262763307295	23.090266139653057
44	20.647921958905098	31.31928139503995	28.255129992850925	19.777666653204033
45	20.013790180242484	34.16059147881779	24.69095964581887	21.13465869512085
46	22.904107033783028	32.191410366534825	24.735261432588214	20.16922116709393
47	22.15235068028641	29.39956505971373	26.8200269058408	21.628057354159058
48	22.818876058673222	26.860664635057784	29.697769380053952	20.622689926215042
49	20.29683862616162	26.291403329878847	30.441822885287102	22.969935158672428
50	19.09777579215467	30.94334076276518	28.899671025923624	21.059212419156527
51	20.013457084101365	31.53850029291642	25.47585906535721	22.97218355762501
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2123.0
1	3135.5
2	4148.0
3	13194.0
4	22240.0
5	16565.5
6	10891.0
7	10557.5
8	10224.0
9	10371.5
10	10519.0
11	10401.0
12	10283.0
13	10044.5
14	9806.0
15	9229.0
16	8652.0
17	8369.0
18	8086.0
19	9160.0
20	10234.0
21	10615.0
22	10996.0
23	11205.5
24	11415.0
25	15252.5
26	22010.0
27	24930.0
28	32865.5
29	40801.0
30	47933.0
31	55065.0
32	60488.5
33	65912.0
34	69559.0
35	73206.0
36	84090.0
37	94974.0
38	103200.0
39	111426.0
40	142512.5
41	173599.0
42	197387.5
43	221176.0
44	220247.0
45	219318.0
46	209329.5
47	199341.0
48	186492.0
49	173643.0
50	159716.5
51	145790.0
52	136041.0
53	126292.0
54	121043.0
55	115794.0
56	106044.5
57	96295.0
58	95615.0
59	94935.0
60	87912.0
61	80889.0
62	69001.0
63	57113.0
64	44349.0
65	31585.0
66	26089.5
67	20594.0
68	16935.5
69	13277.0
70	10629.5
71	7982.0
72	6153.0
73	4324.0
74	3177.0
75	1646.0
76	1262.0
77	928.0
78	594.0
79	442.5
80	291.0
81	299.0
82	307.0
83	191.5
84	76.0
85	67.0
86	58.0
87	43.5
88	29.0
89	20.5
90	12.0
91	18.5
92	25.0
93	40.0
94	55.0
95	28.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2401709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.32683638136533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.80481835642702	12.53809066156268
2	8.82126102882814	2.7040404893232655
3	2.5635199270505464	1.1787195144681992
4	1.1575756318966313	0.7096788923654099
5	0.6097598957206587	0.46728450768144614
6	0.3867244429259775	0.3556357356840667
7	0.2844310557124492	0.3051599776878601
8	0.19278996161067974	0.2363888158061273
9	0.16532129492111883	0.2280467193850288
>10	1.4228005967499737	5.074186857691059
>50	0.68891582280519	7.844253804741346
>100	1.8750924315965694	64.20707764071678
>500	0.021537118653059257	2.182685974725253
>1k	0.005179813346938303	1.4406805467877557
>5k	0.0	0.0
>10k+	2.726217551020159E-4	0.5280698613736958
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12638	0.5262086289388098	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4402	0.18328615165284387	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3878	0.16146835440929772	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3545	0.14760322753505942	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2610	0.10867261604132723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6654807056142105E-4	0.0	0.0	0.022400715490511133	0.0
2	1.6654807056142105E-4	0.0	0.0	0.08710464090362321	0.0
3	1.6654807056142105E-4	0.0	0.0	0.12545233415039042	0.0
4	1.6654807056142105E-4	0.0	0.0	0.23279256562722628	0.0
5	1.6654807056142105E-4	0.0	0.0	0.3978833405712349	0.0
6	1.6654807056142105E-4	0.0	0.0	0.5708018748316304	0.0
7	1.6654807056142105E-4	0.0	0.0	0.6609043810053591	0.0
8	1.6654807056142105E-4	0.0	0.0	0.861303346908389	0.0
9	1.6654807056142105E-4	0.0	0.0	0.9192204384461231	0.0
10	1.6654807056142105E-4	0.0	0.0	1.0401343376737149	0.0
11	2.081850882017763E-4	0.0	0.0	1.219215150544883	0.0
12	2.081850882017763E-4	0.0	0.0	1.3676511184327493	0.0
13	2.081850882017763E-4	0.0	0.0	1.4236945441766675	0.0
14	2.081850882017763E-4	0.0	0.0	1.4479272884433543	0.0
15	2.081850882017763E-4	0.0	0.0	1.48727427011349	0.0
16	2.081850882017763E-4	0.0	0.0	1.5702152092530777	0.0
17	2.081850882017763E-4	0.0	0.0	1.6725173615954305	0.0
18	2.081850882017763E-4	0.0	0.0	1.8067967434855763	0.0
19	2.081850882017763E-4	0.0	0.0	1.8853657957729266	0.0
20	2.081850882017763E-4	0.0	0.0	1.9652672326247684	0.0
21	2.081850882017763E-4	0.0	0.0	2.0666950075966737	0.0
22	2.081850882017763E-4	0.0	0.0	2.17994769557844	0.0
23	2.081850882017763E-4	0.0	0.0	2.2961149747950316	0.0
24	2.081850882017763E-4	0.0	0.0	2.3808879427107947	0.0
25	2.081850882017763E-4	0.0	0.0	2.452045605858162	0.0
26	2.081850882017763E-4	0.0	0.0	2.5254933049757486	0.0
27	2.081850882017763E-4	0.0	0.0	2.594694028294019	0.0
28	2.081850882017763E-4	0.0	0.0	2.673679450757773	0.0
29	2.081850882017763E-4	0.0	0.0	2.7569534860384834	0.0
30	2.081850882017763E-4	0.0	0.0	2.8591723643455556	0.0
31	2.081850882017763E-4	0.0	0.0	2.961058146511505	0.0
32	2.081850882017763E-4	0.0	0.0	3.053742147778936	0.0
33	2.081850882017763E-4	0.0	0.0	3.1432617357056998	0.0
34	2.081850882017763E-4	0.0	0.0	3.2434404001483945	0.0
35	2.081850882017763E-4	0.0	0.0	3.3614813451588015	0.0
36	2.081850882017763E-4	0.0	0.0	3.4789393719222437	0.0
37	2.081850882017763E-4	0.0	0.0	3.590318394110194	0.0
38	2.081850882017763E-4	0.0	0.0	3.7021970605098287	0.0
39	2.081850882017763E-4	0.0	0.0	3.8179479695500165	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	30	2.1664928E-6	45.000004	1
ATTACGC	30	2.1664928E-6	45.000004	41
GCGCAAC	60	0.0	45.000004	27
CCGTCGA	30	2.1664928E-6	45.000004	24
ATATCGT	30	2.1664928E-6	45.000004	11
CGAGCAA	30	2.1664928E-6	45.000004	33
CACCGTA	45	3.8562575E-10	45.000004	25
TATCGGT	30	2.1664928E-6	45.000004	28
ATACCCG	30	2.1664928E-6	45.000004	22
ACGACTA	45	3.8562575E-10	45.000004	10
TACGTGC	30	2.1664928E-6	45.000004	1
TACCGTA	30	2.1664928E-6	45.000004	45
CTTCGTA	40	6.8212103E-9	45.0	12
AAACTCG	25	3.89208E-5	45.0	39
GGTACGT	20	7.0348236E-4	45.0	9
CGTATTA	50	2.1827873E-11	45.0	13
CCCTCGA	25	3.89208E-5	45.0	23
CGTATCA	25	3.89208E-5	45.0	11
CACGGTA	20	7.0348236E-4	45.0	18
GATCGAC	80	0.0	45.0	9
>>END_MODULE