FastQCFastQC Report
Thu 26 May 2016
SRR1547513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547513_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3674011
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200180.5448541117596001No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC45810.12468661634382695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG302.1668093E-645.00000441
CGTACCG351.2129021E-745.00000420
CGTAAGT302.1668093E-645.00000428
TACGCTA253.8924853E-545.011
CGTAGAT253.8924853E-545.014
GTATCGC551.8189894E-1245.017
CGTCGGT253.8924853E-545.044
TTCGCGA207.035314E-445.023
TACGTAA253.8924853E-545.026
CGTTTTT97950.042.31241
TAACCGG1650.042.2727282
TACGGGA17000.042.2205854
CGCGATA1550.042.09677514
TATTGCG3700.041.9594571
CTCACGA4500.041.524
CGAACGG3650.041.3013732
CCGTCGA603.6379788E-1241.25000429
CACGACG4400.040.90909226
CGCTACG501.0822987E-940.511
CGGTCTA4500.040.531
Produced by FastQC (version 0.11.5)