FastQCFastQC Report
Thu 26 May 2016
SRR1547512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547512_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3683039
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385431.046499914880076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGA207.0353167E-445.029
CGTTTTT185400.042.9611661
CTATGCG1600.042.18751
GCGATCG650.041.5384649
ACGGGAT21750.039.413795
CGAACGG1800.038.7500042
CGAGGGA31750.038.196854
GGGCGAT36000.038.1257
CGGGATA12450.037.5903636
AGGGATT86900.037.517266
TGCGGGA36650.037.510234
CGTACTA301.1404105E-437.50000445
CCCGGTA301.1404105E-437.50000422
TAAGGGA63100.037.119654
TAGACGG4850.037.1134032
TAGGGAT76500.037.0588235
AAGGGAT73650.037.0570265
TACGGGA21800.036.949544
GACCGAT12250.036.918379
ACGGGAC17400.036.853455
Produced by FastQC (version 0.11.5)