##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547512_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3683039
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.592375209711328	31.0	31.0	33.0	30.0	34.0
2	32.020451317512524	33.0	31.0	34.0	30.0	34.0
3	31.9602762827111	33.0	31.0	34.0	30.0	34.0
4	35.754003147943855	37.0	35.0	37.0	35.0	37.0
5	35.734491272017486	37.0	35.0	37.0	33.0	37.0
6	35.805873356214796	37.0	35.0	37.0	35.0	37.0
7	36.14468785152696	37.0	35.0	37.0	35.0	37.0
8	36.156520742788764	37.0	35.0	37.0	35.0	37.0
9	38.01344406073354	39.0	39.0	39.0	35.0	39.0
10	37.50020051376051	39.0	37.0	39.0	35.0	39.0
11	37.155077641045885	39.0	37.0	39.0	33.0	39.0
12	36.85328610421991	39.0	35.0	39.0	33.0	39.0
13	36.739851519356705	39.0	35.0	39.0	33.0	39.0
14	37.9243839123072	40.0	36.0	41.0	33.0	41.0
15	38.03492849247591	40.0	36.0	41.0	33.0	41.0
16	38.06310902491122	40.0	36.0	41.0	33.0	41.0
17	37.96645297538256	40.0	36.0	41.0	33.0	41.0
18	37.81196805138365	39.0	36.0	41.0	33.0	41.0
19	37.75405364971699	39.0	36.0	41.0	33.0	41.0
20	37.62282207709448	39.0	35.0	41.0	33.0	41.0
21	37.51031987442978	39.0	35.0	41.0	33.0	41.0
22	37.436315227723625	39.0	35.0	41.0	33.0	41.0
23	37.35402801870955	39.0	35.0	41.0	32.0	41.0
24	37.24254073877578	39.0	35.0	41.0	32.0	41.0
25	37.16473298273518	39.0	35.0	41.0	32.0	41.0
26	37.132972526220875	39.0	35.0	41.0	32.0	41.0
27	37.09817164575232	39.0	35.0	41.0	32.0	41.0
28	37.05995646529944	39.0	35.0	41.0	32.0	41.0
29	37.01683256680149	39.0	35.0	41.0	32.0	41.0
30	36.94992694891366	39.0	35.0	41.0	31.0	41.0
31	36.86585425785608	39.0	35.0	41.0	31.0	41.0
32	36.70247477694372	39.0	35.0	41.0	31.0	41.0
33	36.55042751380042	39.0	35.0	41.0	30.0	41.0
34	36.36126633467634	39.0	35.0	41.0	30.0	41.0
35	36.152456164596686	39.0	35.0	41.0	29.0	41.0
36	36.03453506737235	39.0	35.0	41.0	29.0	41.0
37	35.996268027571794	39.0	35.0	41.0	29.0	41.0
38	35.8714276443991	39.0	35.0	41.0	28.0	41.0
39	35.80118945251462	39.0	35.0	41.0	27.0	41.0
40	35.68457814321271	39.0	35.0	41.0	27.0	41.0
41	35.61684087515771	39.0	35.0	41.0	26.0	41.0
42	35.57786246629482	39.0	35.0	41.0	26.0	41.0
43	35.493910870886786	38.0	35.0	41.0	26.0	41.0
44	35.36607432069006	38.0	35.0	41.0	26.0	41.0
45	35.27938829863056	38.0	34.0	40.0	25.0	41.0
46	35.21807100060575	38.0	34.0	40.0	25.0	41.0
47	35.167996591944856	38.0	34.0	40.0	24.0	41.0
48	35.02875967373682	38.0	34.0	40.0	24.0	41.0
49	34.939706313183216	38.0	34.0	40.0	24.0	41.0
50	34.868842279432826	38.0	34.0	40.0	24.0	41.0
51	34.494961633585746	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	15.0
12	14.0
13	26.0
14	53.0
15	100.0
16	227.0
17	551.0
18	1282.0
19	2376.0
20	4336.0
21	6894.0
22	10885.0
23	16425.0
24	24852.0
25	38002.0
26	54283.0
27	63249.0
28	65059.0
29	69113.0
30	76794.0
31	91081.0
32	111866.0
33	143482.0
34	229821.0
35	342713.0
36	248207.0
37	331100.0
38	547215.0
39	1202660.0
40	350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.232685833628153	24.132217986287955	29.37527949065975	15.259816689424142
2	32.11717280213433	25.153439863113043	29.81630115782103	12.913086176931603
3	27.98865284891091	24.543671679827447	34.431864555330534	13.035810915931108
4	24.586869701895637	27.577307761335135	33.791143672385765	14.044678864383462
5	23.30173533323975	31.092937109816106	31.41136979543252	14.193957761511623
6	22.191918141513028	39.2430001420023	28.29169063917053	10.273391077314143
7	87.52342834273544	3.0758838013933603	7.941023703523096	1.4596641523481015
8	88.29996098330753	2.5497422101693736	7.770186522597236	1.3801102839258557
9	83.62393121549894	5.6451207820498235	8.670964385660863	2.0599836167903733
10	38.19514265257576	40.470817713306865	13.207272581148342	8.12676705296903
11	26.74269808166571	24.225103236756386	32.1682990595538	16.863899622024096
12	26.939845057301863	23.310912537173785	32.19732400335701	17.551918402167342
13	23.875093367189432	26.16928574473417	31.316719698053703	18.638901190022697
14	16.985185332004356	30.549988745707008	32.53307934018619	19.931746582102445
15	17.508421713698933	29.078187876913603	36.862221659884675	16.551168749502786
16	22.70049271810589	27.87754894802906	33.68128874008665	15.740669593778398
17	21.936232551433747	26.7137545923353	30.42267540474049	20.92733745149047
18	21.57069202905535	27.636389405596844	32.43590415415096	18.35701441119684
19	23.617018445908393	28.474474476105193	30.20671244589047	17.70179463209594
20	25.963178777091418	28.181890009853277	29.785158397725358	16.069772815329948
21	24.616926402354142	26.26988201862647	32.72370995799936	16.389481621020032
22	23.302767089895056	22.854740338074077	33.915334591895444	19.927157980135426
23	20.375021823010833	26.645495744139552	33.20244504606114	19.777037386788464
24	19.459907972736644	27.028494675185357	35.80920538718162	17.70239196489638
25	19.595502518436543	28.930212251350042	32.441687421718854	19.03259780849456
26	19.584424710137473	32.03175421167139	30.041251260168572	18.34256981802256
27	18.40708176047009	30.702362912801085	32.59259540830276	18.29795991842606
28	17.239160378155105	29.617660850183775	35.0437505549086	18.099428216752525
29	18.726138930377875	26.995098341342572	35.10685061982781	19.171912108451743
30	19.72800179417052	28.3211500068286	33.02987016971583	18.920978029285056
31	22.63940186351543	27.46517210379798	30.738854516609788	19.156571516076806
32	23.072685355761912	28.121287882099537	31.003853068077746	17.8021736940608
33	22.686428245804617	27.426861350097028	30.69147516493852	19.195235239159835
34	19.846789566985308	28.336897871567473	31.658774180778426	20.15753838066879
35	19.683853469919814	28.30518493016229	32.10519899463459	19.9057626052833
36	22.74735619144951	28.129487632360124	30.254336160980106	18.868820015210268
37	20.346838575426435	29.72564232960878	30.761797526444873	19.16572156851991
38	19.781490231300836	30.630221401402487	29.3497570891864	20.238531278110276
39	19.795337491674676	29.454398935227132	30.794080649159568	19.956182923938627
40	21.78994031830779	28.25859297172797	29.77375477153514	20.17771193842911
41	19.776467205478955	27.928159327120895	30.527045735871926	21.76832773152823
42	20.004186759901266	28.23603008276589	29.674271708770938	22.085511448561906
43	20.754300999799348	27.936820652727274	29.645111007513087	21.663767339960287
44	19.657733735646023	29.21712205599778	30.11059616800148	21.01454804035472
45	19.21269364782724	30.807547788660393	29.477341945062218	20.502416618450145
46	20.61493239686031	29.698083566315752	29.54668685289512	20.140297183928816
47	20.074454818425764	28.77987444607565	30.424548857614596	20.721121877883995
48	20.369292858424796	27.565605468744696	31.92811697079504	20.136984702035466
49	20.59606211066459	27.337831611340523	31.6670553855118	20.399050892483082
50	19.730092458972063	29.414513395052293	30.546187536976937	20.309206608998707
51	19.15757612124118	30.15273528192343	29.476798915243634	21.21288968159175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2106.0
1	3652.5
2	5199.0
3	34513.0
4	63827.0
5	44829.0
6	25831.0
7	23912.5
8	21994.0
9	22396.0
10	22798.0
11	22925.0
12	23052.0
13	22194.5
14	21337.0
15	20599.5
16	19862.0
17	19271.0
18	18680.0
19	18391.0
20	18102.0
21	20213.5
22	22325.0
23	24503.5
24	26682.0
25	33366.5
26	46551.5
27	53052.0
28	65832.5
29	78613.0
30	93119.5
31	107626.0
32	115617.5
33	123609.0
34	139107.5
35	154606.0
36	164266.0
37	173926.0
38	189997.0
39	206068.0
40	220397.5
41	234727.0
42	259191.0
43	283655.0
44	289452.0
45	295249.0
46	286773.0
47	278297.0
48	267687.5
49	257078.0
50	243802.5
51	230527.0
52	214323.0
53	198119.0
54	187569.5
55	177020.0
56	157510.5
57	138001.0
58	126344.0
59	114687.0
60	101747.0
61	88807.0
62	74442.5
63	60078.0
64	49003.0
65	37928.0
66	31683.5
67	25439.0
68	20100.0
69	14761.0
70	11395.0
71	8029.0
72	6652.5
73	5276.0
74	4038.0
75	2123.5
76	1447.0
77	1187.0
78	927.0
79	647.0
80	367.0
81	290.0
82	213.0
83	159.5
84	106.0
85	90.5
86	75.0
87	53.0
88	31.0
89	29.5
90	28.0
91	21.0
92	14.0
93	10.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3683039.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.490670034003255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.11410047968583	14.187399665951702
2	7.229070277009328	2.528825657355813
3	2.2058826876147566	1.1574709866836996
4	1.1867881807055374	0.8303088187590233
5	0.7855338163798589	0.6869756391425708
6	0.5120850675333698	0.5374026567339868
7	0.39733780025501647	0.48647930494080316
8	0.30187474259817226	0.4223993211507438
9	0.25547099598644873	0.402152300465143
>10	2.9552727421063554	12.620503803238273
>50	1.4166909678494024	18.051807056712843
>100	1.636751026146148	46.549926964610144
>500	0.002355912097276021	0.2897365042665075
>1k	6.282432259402724E-4	0.18979705205056419
>5k	0.0	0.0
>10k+	1.570608064850681E-4	1.0588142679381813
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38543	1.046499914880076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.145447278728246E-5	0.0	0.0	0.01596507666630736	0.0
2	8.145447278728246E-5	0.0	0.0	0.05223946854757715	0.0
3	8.145447278728246E-5	0.0	0.0	0.08188889664214796	0.0
4	8.145447278728246E-5	0.0	0.0	0.13087018627823382	0.0
5	8.145447278728246E-5	0.0	0.0	0.2592695868819201	0.0
6	8.145447278728246E-5	0.0	0.0	0.4116166024850674	0.0
7	8.145447278728246E-5	0.0	0.0	0.5054793066269458	0.0
8	8.145447278728246E-5	0.0	0.0	0.6777012135901901	0.0
9	8.145447278728246E-5	0.0	0.0	0.7478878176418985	0.0
10	8.145447278728246E-5	0.0	0.0	0.8620869884896685	0.0
11	8.145447278728246E-5	0.0	0.0	0.9986046848811538	0.0
12	8.145447278728246E-5	0.0	0.0	1.1062331949240831	0.0
13	8.145447278728246E-5	0.0	0.0	1.151467578811954	0.0
14	8.145447278728246E-5	0.0	0.0	1.1766098594122951	0.0
15	8.145447278728246E-5	0.0	0.0	1.2079155284535408	0.0
16	8.145447278728246E-5	0.0	0.0	1.266236930969235	0.0
17	8.145447278728246E-5	0.0	0.0	1.345274921063828	0.0
18	8.145447278728246E-5	0.0	0.0	1.4376442932046063	0.0
19	8.145447278728246E-5	0.0	0.0	1.5025635080160704	0.0
20	8.145447278728246E-5	0.0	0.0	1.5709309621755294	0.0
21	8.145447278728246E-5	0.0	0.0	1.6546390087099268	0.0
22	8.145447278728246E-5	0.0	0.0	1.7609099442064013	0.0
23	8.145447278728246E-5	0.0	0.0	1.8634068224637317	0.0
24	8.145447278728246E-5	0.0	0.0	1.9441553564868577	0.0
25	8.145447278728246E-5	0.0	0.0	2.0182246237414265	0.0
26	1.086059637163766E-4	0.0	0.0	2.0885469852477803	0.0
27	1.086059637163766E-4	0.0	0.0	2.164245341958095	0.0
28	1.086059637163766E-4	0.0	0.0	2.2471931467464774	0.0
29	1.086059637163766E-4	0.0	0.0	2.342712091835031	0.0
30	1.3575745464547076E-4	0.0	0.0	2.4519153883518476	0.0
31	1.3575745464547076E-4	0.0	0.0	2.54925348333265	0.0
32	1.3575745464547076E-4	0.0	0.0	2.6419758248555065	0.0
33	1.3575745464547076E-4	0.0	0.0	2.73358495525027	0.0
34	1.3575745464547076E-4	0.0	0.0	2.8359732275438843	0.0
35	1.3575745464547076E-4	0.0	0.0	2.952480275120627	0.0
36	1.3575745464547076E-4	0.0	0.0	3.057556545016222	0.0
37	1.3575745464547076E-4	0.0	0.0	3.170180929390104	0.0
38	1.3575745464547076E-4	0.0	0.0	3.293231486280759	0.0
39	1.3575745464547076E-4	0.0	0.0	3.452800798471045	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGA	20	7.0353167E-4	45.0	29
CGTTTTT	18540	0.0	42.961166	1
CTATGCG	160	0.0	42.1875	1
GCGATCG	65	0.0	41.538464	9
ACGGGAT	2175	0.0	39.41379	5
CGAACGG	180	0.0	38.750004	2
CGAGGGA	3175	0.0	38.19685	4
GGGCGAT	3600	0.0	38.125	7
CGGGATA	1245	0.0	37.590363	6
AGGGATT	8690	0.0	37.51726	6
TGCGGGA	3665	0.0	37.51023	4
CGTACTA	30	1.1404105E-4	37.500004	45
CCCGGTA	30	1.1404105E-4	37.500004	22
TAAGGGA	6310	0.0	37.11965	4
TAGACGG	485	0.0	37.113403	2
TAGGGAT	7650	0.0	37.058823	5
AAGGGAT	7365	0.0	37.057026	5
TACGGGA	2180	0.0	36.94954	4
GACCGAT	1225	0.0	36.91837	9
ACGGGAC	1740	0.0	36.85345	5
>>END_MODULE