FastQCFastQC Report
Thu 26 May 2016
SRR1547511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547511_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2856287
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123160.4311891627136909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG302.1666383E-645.00000414
ACCGCGA253.8922655E-545.00000430
GCAACCG253.8922655E-545.00000412
CGTTCGA253.8922655E-545.00000414
TTAGTCG302.1666383E-645.00000421
TAGTCGT302.1666383E-645.00000422
AGTCGAT253.8922655E-545.00000415
CTAACGA351.2127748E-745.036
GTGCGAT207.0350495E-445.027
TAATCGC207.0350495E-445.027
TATCGCG1350.043.3333321
CACGACG4300.042.90697526
CGACGGT4350.041.89655328
CGTTTTT62750.041.3067741
ACGCGAA603.6379788E-1241.25000421
CGTTAGG4700.041.170212
CGTAACG501.0822987E-940.50000422
TAGTACG1450.040.3448261
GACGGTC4550.040.05494329
ACGGGAT15400.040.0324675
Produced by FastQC (version 0.11.5)