FastQCFastQC Report
Thu 26 May 2016
SRR1547510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547510_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3164389
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171060.5405782917334121No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG48950.15469021033760388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCG453.8562575E-1045.029
CGCGATC207.035164E-445.012
CTCGACA351.2128294E-745.044
CGTAAAC406.8212103E-945.034
CCGCGAC207.035164E-445.011
ACTCCCG207.035164E-445.036
TATACGT207.035164E-445.01
TATACGA253.8923623E-545.017
TAAGCGT253.8923623E-545.021
TGCGTTA406.8212103E-945.030
TCCGCGA302.1667129E-644.99999630
CCGCGAA302.1667129E-644.99999631
ATACGCC302.1667129E-644.99999641
TATACGC302.1667129E-644.99999640
TGTCGCG600.044.9999961
CGGTCTA2850.044.21052631
TGTTGCG1600.043.593751
CGACGGT2850.042.6315828
CCGTACA700.041.78571342
TAAACGG2650.041.6037752