##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547510_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3164389
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.635202245994407	31.0	31.0	33.0	30.0	34.0
2	32.066308535391826	33.0	31.0	34.0	30.0	34.0
3	32.11354229837103	33.0	31.0	34.0	30.0	34.0
4	35.832029816814554	37.0	35.0	37.0	35.0	37.0
5	35.77042771922162	37.0	35.0	37.0	35.0	37.0
6	35.83121449354046	37.0	35.0	37.0	35.0	37.0
7	36.17812411811569	37.0	35.0	37.0	35.0	37.0
8	36.196489432873136	37.0	35.0	37.0	35.0	37.0
9	38.05342295147657	39.0	39.0	39.0	35.0	39.0
10	37.52990008497691	39.0	37.0	39.0	35.0	39.0
11	37.12324970160116	39.0	37.0	39.0	33.0	39.0
12	36.64397961186188	39.0	35.0	39.0	33.0	39.0
13	36.441213137828505	39.0	35.0	39.0	32.0	39.0
14	37.60779316322993	40.0	35.0	41.0	33.0	41.0
15	37.772481512228744	40.0	35.0	41.0	33.0	41.0
16	37.84823420887887	40.0	35.0	41.0	33.0	41.0
17	37.782940403344845	40.0	35.0	41.0	33.0	41.0
18	37.64428203991355	39.0	36.0	41.0	33.0	41.0
19	37.62850174235848	39.0	36.0	41.0	33.0	41.0
20	37.52987385558476	39.0	35.0	41.0	33.0	41.0
21	37.42729639118326	39.0	35.0	41.0	33.0	41.0
22	37.32923164629886	39.0	35.0	41.0	32.0	41.0
23	37.27167677551654	39.0	35.0	41.0	32.0	41.0
24	37.155122837299714	39.0	35.0	41.0	32.0	41.0
25	37.07693270328016	39.0	35.0	41.0	32.0	41.0
26	37.035897609301514	39.0	35.0	41.0	32.0	41.0
27	37.01689141252861	39.0	35.0	41.0	32.0	41.0
28	36.99911294091845	39.0	35.0	41.0	32.0	41.0
29	36.99858298079029	39.0	35.0	41.0	32.0	41.0
30	36.95682484043523	39.0	35.0	41.0	32.0	41.0
31	36.88481883864468	39.0	35.0	41.0	31.0	41.0
32	36.73549522514457	39.0	35.0	41.0	31.0	41.0
33	36.66845131872219	39.0	35.0	41.0	31.0	41.0
34	36.59965446726051	39.0	35.0	41.0	31.0	41.0
35	36.45885161400827	39.0	35.0	41.0	30.0	41.0
36	36.33175978048211	39.0	35.0	41.0	30.0	41.0
37	36.28040705488485	39.0	35.0	41.0	30.0	41.0
38	36.16555707910753	39.0	35.0	41.0	30.0	41.0
39	36.135188499264785	38.0	35.0	41.0	30.0	41.0
40	35.997021225898585	38.0	35.0	41.0	29.0	41.0
41	35.980085255004994	38.0	35.0	41.0	29.0	41.0
42	35.969127057387695	38.0	35.0	41.0	29.0	41.0
43	35.86739525387049	38.0	35.0	41.0	29.0	41.0
44	35.733035982617814	38.0	35.0	40.0	29.0	41.0
45	35.63617462960464	38.0	35.0	40.0	28.0	41.0
46	35.572693812296784	38.0	35.0	40.0	28.0	41.0
47	35.507598149279374	38.0	35.0	40.0	28.0	41.0
48	35.39959499290384	38.0	34.0	40.0	27.0	41.0
49	35.323099656837385	38.0	34.0	40.0	27.0	41.0
50	35.22379707425351	38.0	34.0	40.0	27.0	41.0
51	34.86345989699749	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	11.0
13	8.0
14	26.0
15	63.0
16	144.0
17	362.0
18	825.0
19	1752.0
20	3032.0
21	5109.0
22	8061.0
23	11895.0
24	17302.0
25	25910.0
26	35875.0
27	42683.0
28	48315.0
29	54013.0
30	63962.0
31	78173.0
32	97416.0
33	128533.0
34	226539.0
35	374090.0
36	200805.0
37	270287.0
38	453781.0
39	1015020.0
40	397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.032151862492253	24.499832353101976	30.394556421476626	14.073459362929148
2	28.721152803906218	26.511310714327475	31.77302158489364	12.994514896872666
3	28.455445901246655	25.69409133959194	32.97205242465449	12.878410334506915
4	26.01348949196828	27.059125790160437	32.64285775231806	14.284526965553223
5	23.29814697244871	32.63795949233801	30.74422898069738	13.319664554515894
6	22.27763400770259	41.24805768190952	26.256032365173816	10.218275945214069
7	90.41138747480161	3.3353042246070252	5.050927683037705	1.2023806175536573
8	91.52594703116462	2.6685088337748613	4.723407899597679	1.0821362354628334
9	87.03411622275264	5.871022810406685	5.562716846759359	1.5321441200813175
10	45.15260291955256	38.35678862491306	9.78782949883848	6.702778956695905
11	33.24360563761282	23.209915089453286	28.28994791727566	15.256531355658232
12	32.98918053374601	22.673950642604307	27.87874689237006	16.458121931279624
13	22.987565688036458	31.856292004554433	27.140531710861087	18.015610596548022
14	16.267721825603616	35.540952771609305	29.507054916446744	18.68427048634033
15	16.21333533898645	29.222292202380935	38.3576734718772	16.206698986755423
16	19.142368400345216	26.381143405567393	37.75395502891711	16.722533165170276
17	19.99302234965423	26.16470351780391	27.20326104028297	26.639013092258885
18	20.945117683066147	28.96783549683683	32.1293936997	17.95765312039702
19	25.232770054503412	27.577582907790415	28.65339880779512	18.536248229911052
20	28.26403454189735	27.1526035515861	28.29159120449477	16.29177070202178
21	24.251158754502054	27.729681780590187	29.685762401525224	18.333397063382538
22	24.007035797431982	24.019676468348234	29.902012679224967	22.071275054994818
23	20.300727881433033	27.9264654250789	29.96398356839188	21.80882312509619
24	20.732248784836504	26.5958135994026	34.96441177111917	17.70752584464173
25	19.210217201488184	29.667559835405825	31.175686680746267	19.946536282359723
26	18.782425296005012	35.6579105792619	27.13054558083725	18.429118543895836
27	18.955886902653248	34.51484630998275	29.420403117315853	17.10886367004815
28	17.314242970759917	30.710952414510352	34.95167629517104	17.02312831955869
29	17.901275728110544	26.56936299551035	35.003945469409736	20.52541580696937
30	19.328723491328027	29.58403660232671	33.005992626064625	18.08124728028065
31	26.43372859657899	27.111647777817456	27.597934387965577	18.856689237637976
32	24.560760386918297	28.920148565805277	29.001933706633416	17.51715734064301
33	23.10161614137832	30.7165143097135	27.870309244533466	18.311560304374716
34	20.085773272502212	29.866018368790943	29.24081078527324	20.807397573433608
35	20.777818403489583	28.292918474940976	30.40492177162795	20.524341349941487
36	24.988109868919402	28.770893843961666	28.663195327755215	17.577800959363717
37	21.152614296156383	31.4205996797486	30.326992035429274	17.09979398866574
38	20.05587808578528	31.525928070158255	27.45579004351235	20.96240380054412
39	21.397464091804135	30.126005367860902	28.629444736408832	19.847085803926127
40	22.041095453182276	29.099772499525184	28.80461283363076	20.054519213661784
41	17.970104181249525	28.27240898637936	30.880369006465386	22.877117825905728
42	21.58508324987857	28.463156710505565	27.843163403740817	22.108596635875045
43	22.42442379871754	27.647517419634564	27.966820766979026	21.961238014668865
44	19.45914361350643	31.993190470577414	29.01605965638232	19.53160625953383
45	19.37012168857874	34.746328596136564	26.129404444270282	19.754145271014405
46	22.228619806224835	31.364443499203166	27.204872725824796	19.202063968747204
47	21.626386642097415	29.951753719280404	27.923494867413584	20.498364771208596
48	22.05370452242123	26.9830921545992	30.41200054734105	20.55120277563852
49	20.67618740932294	28.3026201898692	29.53846698367363	21.48272541713424
50	18.329257243657466	31.655874167177295	28.89268670823973	21.12218188092551
51	18.73726649915671	32.17689102066782	27.387909640692087	21.69793283948339
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2248.0
1	3864.5
2	5481.0
3	17177.0
4	28873.0
5	21123.0
6	13373.0
7	12636.5
8	11900.0
9	11983.5
10	12067.0
11	11999.0
12	11931.0
13	11746.0
14	11561.0
15	11187.5
16	10814.0
17	10283.5
18	9753.0
19	10203.0
20	10653.0
21	14247.5
22	17842.0
23	19775.0
24	21708.0
25	26546.5
26	41974.5
27	52564.0
28	62429.5
29	72295.0
30	76421.5
31	80548.0
32	97478.5
33	114409.0
34	120826.5
35	127244.0
36	134676.5
37	142109.0
38	151245.5
39	160382.0
40	198091.0
41	235800.0
42	256093.0
43	276386.0
44	279245.0
45	282104.0
46	275367.5
47	268631.0
48	251241.0
49	233851.0
50	214539.0
51	195227.0
52	180252.5
53	165278.0
54	148691.0
55	132104.0
56	124886.0
57	117668.0
58	113029.5
59	108391.0
60	97462.0
61	86533.0
62	67162.0
63	47791.0
64	37973.5
65	28156.0
66	23356.0
67	18556.0
68	13393.5
69	8231.0
70	6345.0
71	4459.0
72	3659.5
73	2860.0
74	2246.5
75	1266.0
76	899.0
77	606.5
78	314.0
79	210.0
80	106.0
81	118.0
82	130.0
83	87.5
84	45.0
85	52.0
86	59.0
87	39.0
88	19.0
89	13.5
90	8.0
91	6.5
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3164389.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.016483819920948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.31194520849266	12.210195095896289
2	8.610770729039796	2.5860699865935
3	2.5445793208211476	1.1463190259884857
4	1.1536685504240658	0.6929618048397855
5	0.662914007366337	0.4977318732807778
6	0.42916406009390906	0.3866721098695059
7	0.2981919069600657	0.31344557622680397
8	0.22342038812024004	0.2683990914598434
9	0.17250890769008867	0.23314294990084075
>10	1.759331269514585	6.202762285307416
>50	0.805557887650242	8.986227766710387
>100	2.009765580067872	63.67510853747286
>500	0.015010872638305323	1.4545646005091
>1k	0.0029598903793841484	0.8033186678601746
>5k	0.0	0.0
>10k+	2.1142074138458207E-4	0.5430806280842645
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17106	0.5405782917334121	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4895	0.15469021033760388	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.320335458124776E-5	0.0	0.0	0.021394335525752366	0.0
2	6.320335458124776E-5	0.0	0.0	0.07587562717478794	0.0
3	6.320335458124776E-5	0.0	0.0	0.10668726253314621	0.0
4	6.320335458124776E-5	0.0	0.0	0.17026983724188144	0.0
5	6.320335458124776E-5	0.0	0.0	0.29986831581073	0.0
6	6.320335458124776E-5	0.0	0.0	0.4179637838457914	0.0
7	6.320335458124776E-5	0.0	0.0	0.4883407191720108	0.0
8	6.320335458124776E-5	0.0	0.0	0.659969428537389	0.0
9	6.320335458124776E-5	0.0	0.0	0.7191277684254369	0.0
10	6.320335458124776E-5	0.0	0.0	0.8436067752732044	0.0
11	6.320335458124776E-5	0.0	0.0	1.0418756985945785	0.0
12	6.320335458124776E-5	0.0	0.0	1.1879070493545516	0.0
13	6.320335458124776E-5	0.0	0.0	1.2448532718322558	0.0
14	6.320335458124776E-5	0.0	0.0	1.2688389448958393	0.0
15	6.320335458124776E-5	0.0	0.0	1.3051808737800568	0.0
16	6.320335458124776E-5	0.0	0.0	1.3896205555006038	0.0
17	6.320335458124776E-5	0.0	0.0	1.4952333610058688	0.0
18	6.320335458124776E-5	0.0	0.0	1.616457395092702	0.0
19	6.320335458124776E-5	0.0	0.0	1.6972312822475366	0.0
20	6.320335458124776E-5	0.0	0.0	1.777183525792815	0.0
21	6.320335458124776E-5	0.0	0.0	1.8749591153300054	0.0
22	6.320335458124776E-5	0.0	0.0	1.9994381221777726	0.0
23	6.320335458124776E-5	0.0	0.0	2.120914969682931	0.0
24	6.320335458124776E-5	0.0	0.0	2.2130654606623903	0.0
25	6.320335458124776E-5	0.0	0.0	2.2937129411080623	0.0
26	6.320335458124776E-5	0.0	0.0	2.3742656165218623	0.0
27	6.320335458124776E-5	0.0	0.0	2.4484347531229567	0.0
28	6.320335458124776E-5	0.0	0.0	2.5333800616801536	0.0
29	6.320335458124776E-5	0.0	0.0	2.632261709922516	0.0
30	6.320335458124776E-5	0.0	0.0	2.7511788215671333	0.0
31	6.320335458124776E-5	0.0	0.0	2.859856989769589	0.0
32	6.320335458124776E-5	0.0	0.0	2.9615511872908167	0.0
33	6.320335458124776E-5	0.0	0.0	3.0564194225172696	0.0
34	6.320335458124776E-5	0.0	0.0	3.160736559253619	0.0
35	6.320335458124776E-5	0.0	0.0	3.281802584953999	0.0
36	6.320335458124776E-5	0.0	0.0	3.396516673518964	0.0
37	6.320335458124776E-5	0.0	0.0	3.517645902573925	0.0
38	6.320335458124776E-5	0.0	0.0	3.6415560792304613	0.0
39	9.480503187187164E-5	0.0	0.0	3.7674887632335974	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTCG	45	3.8562575E-10	45.0	29
CGCGATC	20	7.035164E-4	45.0	12
CTCGACA	35	1.2128294E-7	45.0	44
CGTAAAC	40	6.8212103E-9	45.0	34
CCGCGAC	20	7.035164E-4	45.0	11
ACTCCCG	20	7.035164E-4	45.0	36
TATACGT	20	7.035164E-4	45.0	1
TATACGA	25	3.8923623E-5	45.0	17
TAAGCGT	25	3.8923623E-5	45.0	21
TGCGTTA	40	6.8212103E-9	45.0	30
TCCGCGA	30	2.1667129E-6	44.999996	30
CCGCGAA	30	2.1667129E-6	44.999996	31
ATACGCC	30	2.1667129E-6	44.999996	41
TATACGC	30	2.1667129E-6	44.999996	40
TGTCGCG	60	0.0	44.999996	1
CGGTCTA	285	0.0	44.210526	31
TGTTGCG	160	0.0	43.59375	1
CGACGGT	285	0.0	42.63158	28
CCGTACA	70	0.0	41.785713	42
TAAACGG	265	0.0	41.603775	2
>>END_MODULE