FastQCFastQC Report
Thu 26 May 2016
SRR1547509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547509_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2789690
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153210.5492008072581541No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37820.13557061895766195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG302.16662E-645.00000433
GATCGAC406.8212103E-945.09
CGAAACG207.035021E-445.013
CCGTAAA207.035021E-445.034
CGCTAAA351.2127566E-745.019
TCATCGT351.2127566E-745.034
CGCTTAT207.035021E-445.022
TATCCGT207.035021E-445.025
CGTATCG253.892244E-544.99999610
CGTTACG650.044.99999625
CGTCGAA253.892244E-544.99999631
CGGTCGA253.892244E-544.99999637
ATACGCG502.1827873E-1144.9999961
GTACGAT253.892244E-544.99999612
TATCGCG1700.042.3529431
CGACGGT5100.041.91176628
ACGGTCT5100.041.91176630
CGTTTTT79800.041.7293241
TCGATAG1150.041.086961
CCTTACG556.184564E-1140.90909233
Produced by FastQC (version 0.11.5)