##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547509_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2789690
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.608561166294464	31.0	31.0	33.0	30.0	34.0
2	32.037122404281476	33.0	31.0	34.0	30.0	34.0
3	32.086806060888485	33.0	31.0	34.0	30.0	34.0
4	35.82507124447519	37.0	35.0	37.0	35.0	37.0
5	35.77819901135968	37.0	35.0	37.0	35.0	37.0
6	35.82891073918607	37.0	35.0	37.0	35.0	37.0
7	36.134421387322604	37.0	35.0	37.0	35.0	37.0
8	36.13838921170453	37.0	35.0	37.0	35.0	37.0
9	37.98236649950353	39.0	38.0	39.0	35.0	39.0
10	37.486204918826104	39.0	37.0	39.0	35.0	39.0
11	37.13933662880105	39.0	37.0	39.0	33.0	39.0
12	36.51748258767103	39.0	35.0	39.0	33.0	39.0
13	36.25222300685739	39.0	35.0	39.0	32.0	39.0
14	37.300322616491435	40.0	35.0	41.0	32.0	41.0
15	37.495211654341524	40.0	35.0	41.0	33.0	41.0
16	37.59195717086845	40.0	35.0	41.0	33.0	41.0
17	37.52174148382078	39.0	35.0	41.0	33.0	41.0
18	37.41127329559915	39.0	35.0	41.0	33.0	41.0
19	37.39594901225584	39.0	35.0	41.0	33.0	41.0
20	37.258473522147625	39.0	35.0	41.0	32.0	41.0
21	37.13358007520549	39.0	35.0	41.0	32.0	41.0
22	37.09111370797472	39.0	35.0	41.0	32.0	41.0
23	37.01981689721797	39.0	35.0	41.0	32.0	41.0
24	36.91225297434482	38.0	35.0	41.0	32.0	41.0
25	36.81516476741143	38.0	35.0	41.0	32.0	41.0
26	36.766472618821446	38.0	35.0	41.0	32.0	41.0
27	36.729320462130204	38.0	35.0	41.0	31.0	41.0
28	36.72266129928415	38.0	35.0	41.0	31.0	41.0
29	36.71449802666246	38.0	35.0	41.0	31.0	41.0
30	36.67446562162821	38.0	35.0	41.0	31.0	41.0
31	36.54014496234348	38.0	35.0	41.0	31.0	41.0
32	36.41247844742606	38.0	35.0	41.0	31.0	41.0
33	36.29858156282598	38.0	35.0	41.0	30.0	41.0
34	36.17595037441436	38.0	35.0	41.0	30.0	41.0
35	35.99610852818772	38.0	35.0	41.0	30.0	41.0
36	35.874016109316806	38.0	35.0	41.0	29.0	41.0
37	35.81861461309321	38.0	35.0	41.0	29.0	41.0
38	35.6898816714402	38.0	35.0	41.0	29.0	41.0
39	35.61601755033713	38.0	35.0	41.0	28.0	41.0
40	35.44452609429722	38.0	35.0	40.0	27.0	41.0
41	35.403921582684816	38.0	35.0	40.0	27.0	41.0
42	35.37344830429188	38.0	34.0	40.0	27.0	41.0
43	35.268642035494985	38.0	34.0	40.0	27.0	41.0
44	35.14037724621732	38.0	34.0	40.0	26.0	41.0
45	35.057163340729616	38.0	34.0	40.0	26.0	41.0
46	35.0178872921364	37.0	34.0	40.0	26.0	41.0
47	34.945275281482886	37.0	34.0	40.0	26.0	41.0
48	34.80046385082213	37.0	34.0	40.0	25.0	41.0
49	34.74702063670157	37.0	34.0	40.0	25.0	41.0
50	34.60939029067746	37.0	34.0	40.0	24.0	41.0
51	34.22499955192154	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	8.0
11	10.0
12	16.0
13	23.0
14	39.0
15	88.0
16	242.0
17	510.0
18	1089.0
19	2215.0
20	3840.0
21	6164.0
22	9436.0
23	13629.0
24	19372.0
25	27752.0
26	37162.0
27	44677.0
28	47936.0
29	52596.0
30	61011.0
31	73321.0
32	92125.0
33	121305.0
34	216916.0
35	349126.0
36	176130.0
37	232722.0
38	382637.0
39	817352.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.094824156089025	23.672917062469306	29.204391885836777	14.027866895604888
2	30.853320619853818	25.83523617319487	30.39402227487642	12.917420932074892
3	28.991429155210795	24.83655890080977	32.226089637199834	13.945922306779607
4	25.72027716341242	27.682753280830486	31.62182894873624	14.975140607020851
5	24.445404328079466	32.000831633622376	29.462305847603137	14.091458190695025
6	22.817983360158298	40.14955066691998	26.199398499474853	10.83306747344687
7	88.65010090726926	3.5420064594990843	6.313067043291548	1.4948255899401008
8	89.38971713702956	2.9719431191279315	6.151292795973746	1.4870469478687596
9	84.06396409636912	6.091752130165	7.364725112826156	2.4795586606397126
10	45.426624463650086	33.07632747724657	12.74783219640892	8.749215862694422
11	37.63081202570895	21.96950198767605	24.843656463621404	15.556029522993594
12	34.763611727467925	21.68344869860092	27.5952525190971	15.95768705483405
13	24.54301373987791	31.47231412809309	26.658840229559555	17.32583190246945
14	17.530048141549777	35.08543960081586	30.063447909982834	17.321064347651532
15	17.06852732740914	26.616971778226254	41.00236943889823	15.312131455466377
16	22.02506371675706	24.117769357885642	37.62955740602003	16.227609519337275
17	22.11116647369421	24.13540572608426	28.45183515014213	25.301592650079403
18	23.493936602274804	26.551874939509407	31.76514236348842	18.18904609472737
19	27.185637113801175	27.572382594481827	27.453193724033852	17.78878656768315
20	28.639992257204206	27.76631095211296	26.668841340794135	16.9248554498887
21	24.714179711724242	26.17599088070717	31.254189533604094	17.855639873964492
22	23.84996182371518	22.394029444131785	31.123852471063092	22.632156261089943
23	21.165613383565915	26.78760005592019	30.06498929988637	21.981797260627527
24	20.33372883725432	26.18556183661984	35.18416024719592	18.296549078929917
25	20.42764608253964	27.533202613910507	31.561535511114137	20.477615792435717
26	18.86073362990153	33.45844878821661	27.54266603099269	20.138151550889166
27	18.03160924690557	32.43851467367342	31.18826823052024	18.34160784890077
28	16.6057877398564	29.224179030644983	35.866780896802155	18.303252332696466
29	18.02300614046722	27.344758736633818	35.682100878592244	18.950134244306717
30	20.334194838853062	28.399965587574243	32.45070240779441	18.815137165778275
31	26.09591029827687	27.767135416479967	27.813592191247054	18.323362093996106
32	27.12491352085716	27.519652721270106	28.478182163609578	16.87725159426316
33	25.941843000476755	27.862092203793253	27.642067756632454	18.553997039097535
34	20.0172062128767	28.87923030874398	29.486215314246383	21.61734816413293
35	19.567084514766876	28.404410525900726	31.233757155813013	20.794747803519385
36	26.050994913413316	26.667264104613775	28.60260459047421	18.679136391498695
37	20.733844979191236	31.27175420924906	30.06065190039037	17.933748911169342
38	21.13915883126799	31.592040692693452	26.70637239263144	20.562428083407116
39	20.996634034605997	29.745527280808982	28.610885080421124	20.646953604163905
40	23.23182862611975	27.12477013574985	27.52689366918905	22.11650756894135
41	19.30483315350451	26.12383454792468	29.74696113188204	24.82437116668877
42	21.834289831486654	26.329699715739025	27.566324573698154	24.26968587907617
43	21.709795712068367	26.956292634665502	27.898404482218453	23.43550717104768
44	19.759937484093214	30.04090060185899	28.920668604755367	21.278493309292433
45	19.237477999347597	32.55870723987253	26.408955833802324	21.79485892697755
46	21.94623775401568	30.52486118529299	26.8991178231273	20.62978323756403
47	20.837763335711138	29.01616308622105	28.33827414515591	21.8077994329119
48	22.0820234506343	27.380067319307884	30.59960067247615	19.938308557581667
49	21.321078686162263	26.367983539389684	30.78653900612613	21.52439876832193
50	19.953077223634168	30.69215575924207	28.884356326330167	20.4704106907936
51	19.516469571887914	31.61519738752335	27.462083600686814	21.406249439901927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1932.0
1	3524.5
2	5117.0
3	16237.0
4	27357.0
5	20033.5
6	12710.0
7	12091.0
8	11472.0
9	11561.0
10	11650.0
11	11520.0
12	11390.0
13	11125.0
14	10860.0
15	10067.5
16	9275.0
17	9943.0
18	10611.0
19	10831.5
20	11052.0
21	11921.5
22	12791.0
23	15476.0
24	18161.0
25	22801.5
26	30409.5
27	33377.0
28	41063.5
29	48750.0
30	57936.5
31	67123.0
32	74944.0
33	82765.0
34	90844.0
35	98923.0
36	105684.5
37	112446.0
38	125142.0
39	137838.0
40	162411.5
41	186985.0
42	208541.0
43	230097.0
44	235444.0
45	240791.0
46	237786.0
47	234781.0
48	219991.0
49	205201.0
50	192602.0
51	180003.0
52	164410.0
53	148817.0
54	143109.5
55	137402.0
56	127552.5
57	117703.0
58	105731.0
59	93759.0
60	89267.5
61	84776.0
62	71260.5
63	57745.0
64	50525.0
65	43305.0
66	33924.5
67	24544.0
68	20702.5
69	16861.0
70	13437.5
71	10014.0
72	8208.5
73	6403.0
74	5011.5
75	2573.0
76	1526.0
77	1336.0
78	1146.0
79	831.5
80	517.0
81	413.0
82	309.0
83	237.0
84	165.0
85	110.5
86	56.0
87	47.5
88	39.0
89	30.0
90	21.0
91	16.5
92	12.0
93	12.0
94	12.0
95	18.0
96	24.0
97	17.0
98	10.0
99	7.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2789690.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.071748364831457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.83208102740713	13.799449471055613
2	8.881283764707769	3.03238083175508
3	2.999377282761415	1.5361384266748443
4	1.4071759614709118	0.9609181567708468
5	0.7921855554867587	0.6761996230762086
6	0.48157951604260485	0.4932842589321999
7	0.32392744163099063	0.38710054403915306
8	0.22412569472846067	0.306097396999785
9	0.17507036849392407	0.268988154935993
>10	1.4935245369583212	5.890648465051266
>50	0.6809167140256702	8.621292468997469
>100	1.6977837236620448	62.006318712653176
>500	0.008437240480038398	0.8841800030347886
>1k	0.0023202411320105594	0.5853002268195591
>5k	0.0	0.0
>10k+	2.1093101200095996E-4	0.55170325920404
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15321	0.5492008072581541	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3782	0.13557061895766195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0753883047937226E-4	0.0	0.0	0.020934225666651134	0.0
2	1.0753883047937226E-4	0.0	0.0	0.06481006850223502	0.0
3	1.0753883047937226E-4	0.0	0.0	0.09484924848280633	0.0
4	1.0753883047937226E-4	0.0	0.0	0.15858392868024762	0.0
5	1.0753883047937226E-4	0.0	0.0	0.2708903139775387	0.0
6	1.0753883047937226E-4	0.0	0.0	0.38602855514411993	0.0
7	1.0753883047937226E-4	0.0	0.0	0.4507310848158756	0.0
8	1.0753883047937226E-4	0.0	0.0	0.584688621316347	0.0
9	1.0753883047937226E-4	0.0	0.0	0.6273456907398313	0.0
10	1.0753883047937226E-4	0.0	0.0	0.707569658277443	0.0
11	1.0753883047937226E-4	0.0	0.0	0.8549337023110095	0.0
12	1.0753883047937226E-4	0.0	0.0	0.983263373349727	0.0
13	1.0753883047937226E-4	0.0	0.0	1.0293975316253778	0.0
14	1.0753883047937226E-4	0.0	0.0	1.0516580695346078	0.0
15	1.0753883047937226E-4	0.0	0.0	1.0845291053844692	0.0
16	1.0753883047937226E-4	0.0	0.0	1.1586233595847568	0.0
17	1.0753883047937226E-4	0.0	0.0	1.2427545712964523	0.0
18	1.0753883047937226E-4	0.0	0.0	1.3558854209607518	0.0
19	1.0753883047937226E-4	0.0	0.0	1.4254630442809058	0.0
20	1.0753883047937226E-4	0.0	0.0	1.5010986883847308	0.0
21	1.0753883047937226E-4	0.0	0.0	1.5892805293778163	0.0
22	1.0753883047937226E-4	0.0	0.0	1.6894350268309382	0.0
23	1.0753883047937226E-4	0.0	0.0	1.783854119991827	0.0
24	1.0753883047937226E-4	0.0	0.0	1.8635403933770418	0.0
25	1.0753883047937226E-4	0.0	0.0	1.9274901512354419	0.0
26	1.0753883047937226E-4	0.0	0.0	1.9991827048883568	0.0
27	1.0753883047937226E-4	0.0	0.0	2.083421455430532	0.0
28	1.0753883047937226E-4	0.0	0.0	2.1581250963368688	0.0
29	1.0753883047937226E-4	0.0	0.0	2.2575268219766356	0.0
30	1.0753883047937226E-4	0.0	0.0	2.3594019407174276	0.0
31	1.0753883047937226E-4	0.0	0.0	2.4529965695113076	0.0
32	1.0753883047937226E-4	0.0	0.0	2.5391710190021115	0.0
33	1.0753883047937226E-4	0.0	0.0	2.6231229993296745	0.0
34	1.0753883047937226E-4	0.0	0.0	2.7230265728450114	0.0
35	1.0753883047937226E-4	0.0	0.0	2.841964519355197	0.0
36	1.0753883047937226E-4	0.0	0.0	2.943301943943592	0.0
37	1.0753883047937226E-4	0.0	0.0	3.057615720743165	0.0
38	1.0753883047937226E-4	0.0	0.0	3.1929354157630416	0.0
39	1.0753883047937226E-4	0.0	0.0	3.4404898035265568	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	30	2.16662E-6	45.000004	33
GATCGAC	40	6.8212103E-9	45.0	9
CGAAACG	20	7.035021E-4	45.0	13
CCGTAAA	20	7.035021E-4	45.0	34
CGCTAAA	35	1.2127566E-7	45.0	19
TCATCGT	35	1.2127566E-7	45.0	34
CGCTTAT	20	7.035021E-4	45.0	22
TATCCGT	20	7.035021E-4	45.0	25
CGTATCG	25	3.892244E-5	44.999996	10
CGTTACG	65	0.0	44.999996	25
CGTCGAA	25	3.892244E-5	44.999996	31
CGGTCGA	25	3.892244E-5	44.999996	37
ATACGCG	50	2.1827873E-11	44.999996	1
GTACGAT	25	3.892244E-5	44.999996	12
TATCGCG	170	0.0	42.352943	1
CGACGGT	510	0.0	41.911766	28
ACGGTCT	510	0.0	41.911766	30
CGTTTTT	7980	0.0	41.729324	1
TCGATAG	115	0.0	41.08696	1
CCTTACG	55	6.184564E-11	40.909092	33
>>END_MODULE