FastQCFastQC Report
Thu 26 May 2016
SRR1547508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547508_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3864766
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205680.532192634689914No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC58010.15009964380767168No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG1000.045.0000041
GACGCGT253.8925224E-545.00000410
CGTATCG351.2129203E-745.041
TGCGCGA207.0353586E-445.011
CCGACTA351.2129203E-745.010
CTATTCG950.045.01
CCCGGTA453.8562575E-1045.021
GTACGAT453.8562575E-1045.09
TAGTACG1100.042.9545441
GCGATAC1900.042.631589
AATCGCG1550.042.096771
AATGCGG8100.041.9444432
CGTTTTT94900.041.7992631
AACGTAC700.041.78571326
CGCATTA700.041.78571320
ATAGCCG650.041.5384627
CGGTACG650.041.538462
GTACGTC650.041.5384622
TACGGGC6800.041.0294154
CGCCTAA556.184564E-1140.90909245
Produced by FastQC (version 0.11.5)