##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547508_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3864766
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55044833244755	31.0	31.0	33.0	30.0	34.0
2	31.98004924489607	31.0	31.0	34.0	30.0	34.0
3	32.04004640901933	33.0	31.0	34.0	30.0	34.0
4	35.782080208737085	37.0	35.0	37.0	35.0	37.0
5	35.720567558294604	37.0	35.0	37.0	33.0	37.0
6	35.7690602742831	37.0	35.0	37.0	35.0	37.0
7	36.11027084175342	37.0	35.0	37.0	35.0	37.0
8	36.126310105191365	37.0	35.0	37.0	35.0	37.0
9	38.01990728546049	39.0	39.0	39.0	35.0	39.0
10	37.3527830662969	39.0	37.0	39.0	34.0	39.0
11	37.07280078535156	39.0	37.0	39.0	33.0	39.0
12	36.54623902197442	39.0	35.0	39.0	33.0	39.0
13	36.27654662662629	39.0	35.0	39.0	32.0	39.0
14	37.2546045478562	40.0	35.0	41.0	32.0	41.0
15	37.46655761306118	40.0	35.0	41.0	32.0	41.0
16	37.580751331387205	40.0	35.0	41.0	33.0	41.0
17	37.525696510474376	39.0	35.0	41.0	33.0	41.0
18	37.427900680144674	39.0	35.0	41.0	32.0	41.0
19	37.40063020633073	39.0	35.0	41.0	32.0	41.0
20	37.25972387461492	39.0	35.0	41.0	32.0	41.0
21	37.14939559083267	39.0	35.0	41.0	32.0	41.0
22	37.10707220049027	39.0	35.0	41.0	32.0	41.0
23	37.03331301300001	39.0	35.0	41.0	32.0	41.0
24	36.92017783224133	39.0	35.0	41.0	32.0	41.0
25	36.84062036356147	38.0	35.0	41.0	31.0	41.0
26	36.82027889916233	38.0	35.0	41.0	31.0	41.0
27	36.80263902135343	38.0	35.0	41.0	31.0	41.0
28	36.80189382746588	38.0	35.0	41.0	31.0	41.0
29	36.76203112943966	38.0	35.0	41.0	31.0	41.0
30	36.719064750621385	38.0	35.0	41.0	31.0	41.0
31	36.61372383218027	38.0	35.0	41.0	31.0	41.0
32	36.526005196692374	38.0	35.0	41.0	31.0	41.0
33	36.44561869981261	38.0	35.0	41.0	30.0	41.0
34	36.32281048839697	38.0	35.0	41.0	30.0	41.0
35	36.1637237545559	38.0	35.0	41.0	30.0	41.0
36	36.04723183758085	38.0	35.0	41.0	30.0	41.0
37	35.98607755294887	38.0	35.0	41.0	29.0	41.0
38	35.82734452745651	38.0	35.0	41.0	29.0	41.0
39	35.76570923052004	38.0	35.0	41.0	29.0	41.0
40	35.62766956654038	38.0	35.0	40.0	28.0	41.0
41	35.566611277370995	38.0	35.0	40.0	27.0	41.0
42	35.543346996946255	38.0	35.0	40.0	27.0	41.0
43	35.46860818999132	38.0	35.0	40.0	27.0	41.0
44	35.34109490716902	38.0	34.0	40.0	27.0	41.0
45	35.257855973686375	38.0	34.0	40.0	27.0	41.0
46	35.249347308478704	38.0	34.0	40.0	27.0	41.0
47	35.14654004925524	38.0	34.0	40.0	26.0	41.0
48	35.012909449110246	37.0	34.0	40.0	26.0	41.0
49	34.931028941985105	37.0	34.0	40.0	26.0	41.0
50	34.82147276186967	37.0	34.0	40.0	26.0	41.0
51	34.46873730518225	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	4.0
11	8.0
12	12.0
13	19.0
14	30.0
15	104.0
16	258.0
17	706.0
18	1563.0
19	3017.0
20	4964.0
21	7832.0
22	11819.0
23	17214.0
24	24689.0
25	35799.0
26	48621.0
27	58850.0
28	65093.0
29	73156.0
30	85834.0
31	104703.0
32	130147.0
33	169593.0
34	291127.0
35	453686.0
36	249411.0
37	336170.0
38	545741.0
39	1144289.0
40	301.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.82968024454779	24.52922117406332	30.8881831396778	13.752915441711089
2	28.862471880574397	25.80132923959691	32.15382250827087	13.182376371557813
3	28.291441189453643	25.512049112417156	32.727181930290214	13.469327767838985
4	25.455409202006017	26.968722039057475	32.91733574555355	14.658533013382957
5	23.417355669140125	31.92659012214452	31.01763470285135	13.638419505864002
6	21.654868625940097	40.887572494686616	26.632220424211972	10.825338455161322
7	89.71174451441563	3.4097795312833945	5.4509639134685	1.427512040832485
8	91.40589624313607	2.1562236885751944	4.944827190054974	1.4930528782337664
9	87.90345909687676	3.767472597306021	6.278879497490922	2.0501888083262996
10	41.918527538277864	26.172658318770143	17.648183615773892	14.260630527178101
11	34.9921056022538	26.202077952455593	22.94213931710225	15.863677128188355
12	33.473980054678606	22.705255635140652	26.637550630490953	17.183213679689793
13	24.93330773454331	28.86449011401984	26.835880878687092	19.366321272749758
14	17.77810092512716	32.87231361484758	31.140255322055722	18.20933013796954
15	17.26927322378638	26.93166934298221	38.87371188837824	16.92534554485317
16	22.325129128128328	24.742558799161447	36.18012060756072	16.752191465149508
17	22.816801845182866	24.470666529357793	28.24313296070189	24.469398664757453
18	24.076283014288574	26.2865591344987	31.105246734213665	18.531911116999066
19	26.87971276915601	26.992940840402756	26.72203181253406	19.405314577907173
20	27.67000641177241	28.395121464016192	26.696855644041577	17.238016480169822
21	24.85345813950961	26.50201849219332	30.84662823053194	17.79789513776513
22	24.63905964811324	22.208951331076708	31.421980011208955	21.730009009601098
23	20.96856575533939	25.710249986674484	31.475514947088644	21.845669310897478
24	21.05097695436153	26.049882450839196	34.37157644214424	18.52756415265504
25	20.16585221459721	27.672102269581135	31.737341924452867	20.424703591368793
26	19.554508604143177	32.1321911857018	27.614608491173854	20.698691718981173
27	18.933177325612988	31.682978995364792	30.669670557027256	18.714173121994968
28	17.371012889266776	29.363226648133416	34.53789957787871	18.727860884721093
29	18.038815286617613	27.896126182024993	34.07869971946555	19.986358811891847
30	21.10932460076496	28.33447096150194	31.299437016367875	19.256767421365225
31	25.26836036127414	26.83256890585355	28.51988969060481	19.3791810422675
32	25.95934656845977	27.92616681061674	28.316953730187027	17.797532890736463
33	25.042059467507215	27.469787304069637	27.193004699378953	20.29514852904419
34	20.656826312382172	28.32650152687123	30.29179515655023	20.724877004196372
35	20.666788105670562	27.90013677412811	29.855028739126766	21.578046381074557
36	25.761378567292304	26.481292787195915	28.443481442343472	19.313847203168315
37	21.25926899584606	29.770418183144855	29.87994615974163	19.09036666126746
38	21.37089283025156	30.20661535523755	26.70125435796113	21.721237456549762
39	20.35512628707663	29.175039316740005	29.253181175781407	21.21665322040196
40	22.868085674527254	27.399175008266997	28.02205877406291	21.710680543142846
41	20.382243064651263	25.95111838595144	28.465578511092264	25.201060038305034
42	21.370168336194222	26.6963640230741	28.72688799270124	23.206579648030438
43	21.73479584533708	26.058291756861863	29.69882264540725	22.508089752393808
44	21.09568858761436	28.65596519944545	28.91727468105443	21.331071531885758
45	19.238862068233885	31.565740332014926	26.794274219965708	22.401123379785478
46	22.63161598916985	28.755686631480405	27.600739604933388	21.011957774416356
47	20.860253893767435	29.04359539490877	28.139970181894586	21.956180529429208
48	21.080991708165513	27.65577527850328	30.086737463535957	21.176495549795252
49	22.287791809387684	25.07784429898214	30.82328917197057	21.81107471965961
50	20.58543777294667	29.119305023900544	28.764277061017406	21.530980142135384
51	19.661397352388217	31.1082223348063	27.110386502054716	22.119993810750767
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2710.0
1	4657.0
2	6604.0
3	20398.5
4	34193.0
5	24449.5
6	14706.0
7	14119.5
8	13533.0
9	13934.5
10	14336.0
11	14160.5
12	13985.0
13	13373.0
14	12761.0
15	12130.5
16	11500.0
17	11181.5
18	10863.0
19	12105.0
20	13347.0
21	16219.5
22	19092.0
23	21108.0
24	23124.0
25	27665.0
26	38947.5
27	45689.0
28	51191.0
29	56693.0
30	67969.0
31	79245.0
32	90815.0
33	102385.0
34	113311.5
35	124238.0
36	139006.5
37	153775.0
38	169506.0
39	185237.0
40	220235.0
41	255233.0
42	287464.0
43	319695.0
44	331187.5
45	342680.0
46	332914.0
47	323148.0
48	315117.0
49	307086.0
50	286119.0
51	265152.0
52	248983.5
53	232815.0
54	222623.5
55	212432.0
56	199864.0
57	187296.0
58	167827.5
59	148359.0
60	129277.0
61	110195.0
62	93350.0
63	76505.0
64	62359.5
65	48214.0
66	37012.0
67	25810.0
68	21093.5
69	16377.0
70	13570.5
71	10764.0
72	8346.5
73	5929.0
74	4740.5
75	2567.5
76	1583.0
77	1097.5
78	612.0
79	540.5
80	469.0
81	425.5
82	382.0
83	268.5
84	155.0
85	102.0
86	49.0
87	38.0
88	27.0
89	21.0
90	15.0
91	10.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3864766.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.597969653119534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.42081124149317	12.544013732240646
2	9.25540984872923	2.887312038953244
3	2.8513746985274975	1.334269680519143
4	1.3659863757199562	0.8522645614021847
5	0.7691641180393796	0.5998699285723348
6	0.5034579722289235	0.47117533034687087
7	0.3451072696753645	0.3768080903627092
8	0.2643096842862436	0.3298155547617946
9	0.21650872193554788	0.3039386826947734
>10	1.6861449266191146	5.7379372422503145
>50	0.5460230522434109	6.2852902372921164
>100	1.7389450078262148	63.965402280986325
>500	0.03425091794808864	3.1464932610452925
>1k	0.0021720094308544016	0.4782142287797536
>5k	1.6707764852726166E-4	0.15117824202459676
>10k+	1.6707764852726166E-4	0.536016907767955
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20568	0.532192634689914	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5801	0.15009964380767168	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.762436328616015E-5	0.0	0.0	0.02517616849247794	0.0
2	7.762436328616015E-5	0.0	0.0	0.0955814659930252	0.0
3	7.762436328616015E-5	0.0	0.0	0.13736924822874141	0.0
4	7.762436328616015E-5	0.0	0.0	0.24640560385803437	0.0
5	7.762436328616015E-5	0.0	0.0	0.4575179972086279	0.0
6	7.762436328616015E-5	0.0	0.0	0.6677247729875496	0.0
7	7.762436328616015E-5	0.0	0.0	0.7859208034846095	0.0
8	7.762436328616015E-5	0.0	0.0	1.0390021025852536	0.0
9	7.762436328616015E-5	0.0	0.0	1.1088640295427976	0.0
10	7.762436328616015E-5	0.0	0.0	1.2613959034001023	0.0
11	7.762436328616015E-5	0.0	0.0	1.4932857513236248	0.0
12	7.762436328616015E-5	0.0	0.0	1.642686775861721	0.0
13	7.762436328616015E-5	0.0	0.0	1.7021470381389197	0.0
14	1.0349915104821352E-4	0.0	0.0	1.7277113284478285	0.0
15	1.0349915104821352E-4	0.0	0.0	1.7724488364884188	0.0
16	1.0349915104821352E-4	0.0	0.0	1.875430491781391	0.0
17	1.0349915104821352E-4	0.0	0.0	2.005166677620327	0.0
18	1.0349915104821352E-4	0.0	0.0	2.1601049067395026	0.0
19	1.0349915104821352E-4	0.0	0.0	2.249217675792014	0.0
20	1.0349915104821352E-4	0.0	0.0	2.348473361647251	0.0
21	1.0349915104821352E-4	0.0	0.0	2.495985526678717	0.0
22	1.2937393881026692E-4	0.0	0.0	2.6458005478210067	0.0
23	1.2937393881026692E-4	0.0	0.0	2.7985394199804077	0.0
24	1.2937393881026692E-4	0.0	0.0	2.9105513762023367	0.0
25	1.2937393881026692E-4	0.0	0.0	3.0114112988988206	0.0
26	1.2937393881026692E-4	0.0	0.0	3.110304737725389	0.0
27	1.2937393881026692E-4	0.0	0.0	3.2259909138095293	0.0
28	1.2937393881026692E-4	0.0	0.0	3.3418582134080044	0.0
29	1.2937393881026692E-4	0.0	0.0	3.4964341954985114	0.0
30	1.2937393881026692E-4	0.0	0.0	3.663973446257807	0.0
31	1.552487265723203E-4	0.0	0.0	3.8066470259777696	0.0
32	1.552487265723203E-4	0.0	0.0	3.9365384605432774	0.0
33	1.552487265723203E-4	0.0	0.0	4.071941224902103	0.0
34	1.8112351433437367E-4	0.0	0.0	4.218107900969942	0.0
35	1.8112351433437367E-4	0.0	0.0	4.385233155125045	0.0
36	1.8112351433437367E-4	0.0	0.0	4.524827635101323	0.0
37	1.8112351433437367E-4	0.0	0.0	4.6785238744079205	0.0
38	1.8112351433437367E-4	0.0	0.0	4.870928796206549	0.0
39	1.8112351433437367E-4	0.0	0.0	5.239178775636093	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	100	0.0	45.000004	1
GACGCGT	25	3.8925224E-5	45.000004	10
CGTATCG	35	1.2129203E-7	45.0	41
TGCGCGA	20	7.0353586E-4	45.0	11
CCGACTA	35	1.2129203E-7	45.0	10
CTATTCG	95	0.0	45.0	1
CCCGGTA	45	3.8562575E-10	45.0	21
GTACGAT	45	3.8562575E-10	45.0	9
TAGTACG	110	0.0	42.954544	1
GCGATAC	190	0.0	42.63158	9
AATCGCG	155	0.0	42.09677	1
AATGCGG	810	0.0	41.944443	2
CGTTTTT	9490	0.0	41.799263	1
AACGTAC	70	0.0	41.785713	26
CGCATTA	70	0.0	41.785713	20
ATAGCCG	65	0.0	41.53846	27
CGGTACG	65	0.0	41.53846	2
GTACGTC	65	0.0	41.53846	22
TACGGGC	680	0.0	41.029415	4
CGCCTAA	55	6.184564E-11	40.909092	45
>>END_MODULE