FastQCFastQC Report
Thu 26 May 2016
SRR1547507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547507_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3048548
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159260.5224126370980545No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG31400.10299985435689384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGC551.8189894E-1245.035
CGCGAAA207.035124E-445.019
CCCGTAG406.8212103E-945.037
CCCGTAA207.035124E-445.018
CGTAGCA351.2128112E-745.039
CGCTAAT253.8923285E-545.039
CTATTCG453.8562575E-1045.043
TTACGTC253.8923285E-545.032
GACGATT502.1827873E-1145.030
TAGTACG406.8212103E-945.043
CCCGGTA302.1666874E-644.99999619
GCGTATC302.1666874E-644.99999630
CGCTTAT302.1666874E-644.99999623
CGGTCTA3800.043.22368631
TTACGAT650.041.53846413
CCGCGTA556.184564E-1140.90909242
TAGCACG501.0822987E-940.51
CGTTTTT84950.040.444381
ATATCGG950.040.263162
TACGGGA14150.040.2296834