##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547507_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3048548
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.574659477233094	31.0	31.0	33.0	30.0	34.0
2	32.00150727493876	31.0	31.0	34.0	30.0	34.0
3	32.05470112328886	33.0	31.0	34.0	30.0	34.0
4	35.79161981376052	37.0	35.0	37.0	35.0	37.0
5	35.73392218196991	37.0	35.0	37.0	33.0	37.0
6	35.78765530344282	37.0	35.0	37.0	35.0	37.0
7	36.10102547179838	37.0	35.0	37.0	35.0	37.0
8	36.10639556930053	37.0	35.0	37.0	35.0	37.0
9	37.956235886723775	39.0	38.0	39.0	35.0	39.0
10	37.446221611075174	39.0	37.0	39.0	35.0	39.0
11	37.11867256149485	39.0	37.0	39.0	33.0	39.0
12	36.53319219510403	39.0	35.0	39.0	33.0	39.0
13	36.24924882271822	39.0	35.0	39.0	32.0	39.0
14	37.293108391273485	39.0	35.0	41.0	32.0	41.0
15	37.47840480123652	40.0	35.0	41.0	33.0	41.0
16	37.59472542338189	40.0	35.0	41.0	33.0	41.0
17	37.519694293808065	39.0	35.0	41.0	33.0	41.0
18	37.41011425767283	39.0	35.0	41.0	32.0	41.0
19	37.37102581294439	39.0	35.0	41.0	32.0	41.0
20	37.23668218443666	39.0	35.0	41.0	32.0	41.0
21	37.10807538539659	39.0	35.0	41.0	32.0	41.0
22	37.05264375040183	39.0	35.0	41.0	32.0	41.0
23	36.988871751404275	39.0	35.0	41.0	32.0	41.0
24	36.86269397759195	38.0	35.0	41.0	32.0	41.0
25	36.770176162553454	38.0	35.0	41.0	31.0	41.0
26	36.72858947931933	38.0	35.0	41.0	31.0	41.0
27	36.688996204094536	38.0	35.0	41.0	31.0	41.0
28	36.69843085954363	38.0	35.0	41.0	31.0	41.0
29	36.65872736791417	38.0	35.0	41.0	31.0	41.0
30	36.611514071617044	38.0	35.0	41.0	31.0	41.0
31	36.534252043923864	38.0	35.0	41.0	31.0	41.0
32	36.40461295016513	38.0	35.0	41.0	31.0	41.0
33	36.27477376114793	38.0	35.0	41.0	30.0	41.0
34	36.141920350278234	38.0	35.0	41.0	30.0	41.0
35	35.967789255737486	38.0	35.0	41.0	30.0	41.0
36	35.84483432768649	38.0	35.0	41.0	29.0	41.0
37	35.798417476123056	38.0	35.0	41.0	29.0	41.0
38	35.65465657749197	38.0	35.0	41.0	29.0	41.0
39	35.595900408981585	38.0	35.0	40.0	28.0	41.0
40	35.425237194887536	38.0	35.0	40.0	27.0	41.0
41	35.367773116906804	38.0	34.0	40.0	27.0	41.0
42	35.33511757072547	38.0	34.0	40.0	27.0	41.0
43	35.23836724893293	38.0	34.0	40.0	27.0	41.0
44	35.11097578256928	38.0	34.0	40.0	26.0	41.0
45	35.011134809096	37.0	34.0	40.0	26.0	41.0
46	34.98458118422278	37.0	34.0	40.0	26.0	41.0
47	34.88664997238029	37.0	34.0	40.0	26.0	41.0
48	34.77436799420577	37.0	34.0	40.0	25.0	41.0
49	34.68423492101814	37.0	34.0	40.0	24.0	41.0
50	34.5565626652426	36.0	34.0	40.0	24.0	41.0
51	34.15872703988915	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	9.0
12	23.0
13	44.0
14	96.0
15	156.0
16	269.0
17	614.0
18	1335.0
19	2614.0
20	4228.0
21	6582.0
22	10127.0
23	14539.0
24	20850.0
25	29944.0
26	40080.0
27	48083.0
28	52483.0
29	58311.0
30	67920.0
31	82207.0
32	103210.0
33	135444.0
34	237248.0
35	374682.0
36	201524.0
37	266900.0
38	429269.0
39	859509.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.890904128785245	24.053254204952655	28.532271756915094	14.523569909347007
2	28.94991320458133	26.844058220503662	30.563140222820834	13.642888352094177
3	28.80686149603024	26.145233730943385	31.979322615225346	13.068582157801027
4	25.965574430843795	27.086501508259015	32.67604774469682	14.271876316200368
5	24.557559861284783	32.09363933256094	29.78417922237078	13.564621583783493
6	22.27270162713528	40.71233255963167	26.640289081884227	10.374676731348826
7	88.00891440777708	3.657511707212745	6.520350015810806	1.8132238691993696
8	89.1198367222691	3.1021325562202073	6.008335771652603	1.7696949498580965
9	84.40729816292871	5.422384689366872	7.1633446480094785	3.0069724996949367
10	44.62002894492723	31.64391703853769	13.50944777644964	10.226606240085443
11	36.27835284207433	21.98718865505808	25.070000537961022	16.664457964906575
12	34.41897585342268	20.989074142837836	27.540553732465423	17.051396271274065
13	24.47204374016745	29.4833474821456	27.593989007225733	18.450619770461216
14	17.579418136109386	33.71532939615843	30.086093445141753	18.619159022590427
15	16.824042134156983	26.87853364946197	39.99937019197336	16.298054024407683
16	22.135357553825624	23.98420493953187	36.856529731531204	17.0239077751113
17	21.602513721286332	24.176329190158725	28.382429930576787	25.83872715797816
18	23.578175577356827	25.860114388882838	31.12744165419078	19.434268379569552
19	26.95050233750625	26.63517189166777	27.114842869457856	19.299482901368126
20	29.2167943558704	26.759591779430732	26.654459762483647	17.369154102215216
21	25.37778640848036	26.34647707695598	30.48461103449905	17.791125480064608
22	24.63697471714403	21.583717887991266	30.92865849578225	22.850648899082447
23	21.392052872383836	26.81171495413554	29.610457175022336	22.18577499845828
24	21.363809918689157	25.7235247731051	34.42160005353368	18.49106525467206
25	20.733116224510816	26.370554112974435	31.800877007677098	21.095452654837647
26	20.049479293092972	31.76699202374376	27.37447466794028	20.809054015222987
27	18.997831098608255	31.926182562977523	29.971022270274243	19.104964068139978
28	17.916529442869194	28.07972844777251	34.65813889103927	19.345603218319017
29	18.417784466572282	26.10596913678249	35.219291282276025	20.2569551143692
30	20.163796010428573	27.74825917125136	32.569078787672034	19.518866030648034
31	25.869692719288	26.35638343237502	28.342640496393695	19.431283351943286
32	26.68158743113115	27.097326333716904	27.638830026622514	18.582256208529436
33	25.752161356816426	26.057093409715048	28.041939966174063	20.148805267294463
34	20.756340395493197	28.510851723509028	28.898938117425082	21.833869763572693
35	21.1406216992483	27.283349319085676	30.526073396252905	21.049955585413123
36	26.570026123912104	25.83931760300314	28.53994754223978	19.050708730844978
37	21.555114106781325	30.15619239060694	28.91878363076455	19.369909871847188
38	22.010937666062663	30.322763492652893	26.102065639117377	21.564233202167067
39	21.865360164904736	28.96139408006697	28.00359384205202	21.169651912976274
40	23.748486164560966	26.66164351028752	27.936873554229752	21.652996770921764
41	20.532364916019034	25.37234119325003	28.78439178257977	25.310902108151158
42	22.332664599671713	26.17141012705065	27.7547540665261	23.74117120675154
43	22.536335330786986	26.193879840501115	28.02002789524718	23.24975693346472
44	20.984777015156066	28.280840583779554	28.795118200533498	21.939264200530875
45	19.804116582714133	31.963479007055163	26.072346572860262	22.16005783737045
46	22.7620165403333	29.40475268882104	27.580638389161006	20.252592381684657
47	21.30817687633588	27.399371766493424	28.792887630439147	22.499563726731544
48	23.319298236406315	25.89973981055899	29.772731149386527	21.00823080364816
49	22.714616925828295	24.6338912820136	30.634256045829027	22.01723574632907
50	21.12966566378486	29.731695220150705	28.180104102018404	20.95853501404603
51	19.968358707161574	31.130000249299012	27.687508938681628	21.21413210485779
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2228.0
1	4074.0
2	5920.0
3	17134.5
4	28349.0
5	20776.5
6	13204.0
7	12857.0
8	12510.0
9	12845.5
10	13181.0
11	12659.0
12	12137.0
13	12140.5
14	12144.0
15	11240.0
16	10336.0
17	9805.5
18	9275.0
19	10293.0
20	11311.0
21	12555.0
22	13799.0
23	16761.0
24	19723.0
25	23752.0
26	28882.0
27	29983.0
28	38083.5
29	46184.0
30	52297.0
31	58410.0
32	70759.5
33	83109.0
34	91399.0
35	99689.0
36	106761.0
37	113833.0
38	132932.0
39	152031.0
40	172996.0
41	193961.0
42	215323.0
43	236685.0
44	243796.0
45	250907.0
46	243198.5
47	235490.0
48	229298.5
49	223107.0
50	213535.5
51	203964.0
52	191610.5
53	179257.0
54	170273.0
55	161289.0
56	152401.5
57	143514.0
58	132359.0
59	121204.0
60	111247.0
61	101290.0
62	89558.0
63	77826.0
64	65023.5
65	52221.0
66	43632.5
67	35044.0
68	28679.5
69	22315.0
70	18995.0
71	15675.0
72	11887.0
73	8099.0
74	6159.5
75	3894.0
76	3568.0
77	2627.0
78	1686.0
79	1403.0
80	1120.0
81	846.0
82	572.0
83	388.5
84	205.0
85	139.0
86	73.0
87	57.5
88	42.0
89	35.5
90	29.0
91	23.5
92	18.0
93	16.0
94	14.0
95	13.0
96	12.0
97	8.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3048548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.865456115102875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.31151472740639	13.059097246753101
2	8.292003748026938	2.631128431411799
3	2.389813883009742	1.13746461852464
4	1.0346451603614957	0.6566046954567555
5	0.5834914949148012	0.4628679353053276
6	0.4215911666527684	0.4013241691826712
7	0.2738043973740407	0.3040822155462023
8	0.21717328028358307	0.27564425181696994
9	0.17533210731709584	0.25035514687871024
>10	1.7018441218721554	6.229979711351688
>50	0.7222586302433149	8.428947613295263
>100	1.856575281417243	63.40240675054426
>500	0.017458000980447138	1.6710415813403772
>1k	0.0022861667950585538	0.5639163566850138
>5k	0.0	0.0
>10k+	2.0783334500532307E-4	0.5251392759072349
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15926	0.5224126370980545	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG	3140	0.10299985435689384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.280250138754581E-5	0.0	0.0	0.02709486614611284	0.0
2	3.280250138754581E-5	0.0	0.0	0.09598011905995904	0.0
3	3.280250138754581E-5	0.0	0.0	0.1341950331764499	0.0
4	3.280250138754581E-5	0.0	0.0	0.22938789220310785	0.0
5	3.280250138754581E-5	0.0	0.0	0.4427353612277058	0.0
6	3.280250138754581E-5	0.0	0.0	0.5885752823967344	0.0
7	3.280250138754581E-5	0.0	0.0	0.6879340590996107	0.0
8	3.280250138754581E-5	0.0	0.0	0.8521105785442775	0.0
9	3.280250138754581E-5	0.0	0.0	0.9052506307921017	0.0
10	3.280250138754581E-5	0.0	0.0	1.1232888575151188	0.0
11	6.560500277509162E-5	0.0	0.0	1.2806424566711758	0.0
12	6.560500277509162E-5	0.0	0.0	1.4634835994053563	0.0
13	6.560500277509162E-5	0.0	0.0	1.5217408418696377	0.0
14	6.560500277509162E-5	0.0	0.0	1.5481796579879996	0.0
15	6.560500277509162E-5	0.0	0.0	1.5935455174069755	0.0
16	6.560500277509162E-5	0.0	0.0	1.6755517708758398	0.0
17	6.560500277509162E-5	0.0	0.0	1.7714662849330238	0.0
18	6.560500277509162E-5	0.0	0.0	1.895098912662684	0.0
19	6.560500277509162E-5	0.0	0.0	1.9736609034858563	0.0
20	6.560500277509162E-5	0.0	0.0	2.049861114209125	0.0
21	6.560500277509162E-5	0.0	0.0	2.145710023263534	0.0
22	6.560500277509162E-5	0.0	0.0	2.246446505024687	0.0
23	6.560500277509162E-5	0.0	0.0	2.354432339592488	0.0
24	6.560500277509162E-5	0.0	0.0	2.4338471954517362	0.0
25	6.560500277509162E-5	0.0	0.0	2.5020107933350566	0.0
26	6.560500277509162E-5	0.0	0.0	2.5722081463044044	0.0
27	6.560500277509162E-5	0.0	0.0	2.653787967255231	0.0
28	6.560500277509162E-5	0.0	0.0	2.732415563081178	0.0
29	6.560500277509162E-5	0.0	0.0	2.8318399447868297	0.0
30	6.560500277509162E-5	0.0	0.0	2.936676739221426	0.0
31	9.840750416263743E-5	0.0	0.0	3.0263915805163637	0.0
32	9.840750416263743E-5	0.0	0.0	3.1112844541073326	0.0
33	1.3121000555018324E-4	0.0	0.0	3.1961445251969134	0.0
34	1.3121000555018324E-4	0.0	0.0	3.2948800543734262	0.0
35	1.3121000555018324E-4	0.0	0.0	3.400176083827448	0.0
36	1.3121000555018324E-4	0.0	0.0	3.496484227901283	0.0
37	1.3121000555018324E-4	0.0	0.0	3.597122302158273	0.0
38	1.3121000555018324E-4	0.0	0.0	3.7133415645743484	0.0
39	1.3121000555018324E-4	0.0	0.0	3.9008734650069474	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGC	55	1.8189894E-12	45.0	35
CGCGAAA	20	7.035124E-4	45.0	19
CCCGTAG	40	6.8212103E-9	45.0	37
CCCGTAA	20	7.035124E-4	45.0	18
CGTAGCA	35	1.2128112E-7	45.0	39
CGCTAAT	25	3.8923285E-5	45.0	39
CTATTCG	45	3.8562575E-10	45.0	43
TTACGTC	25	3.8923285E-5	45.0	32
GACGATT	50	2.1827873E-11	45.0	30
TAGTACG	40	6.8212103E-9	45.0	43
CCCGGTA	30	2.1666874E-6	44.999996	19
GCGTATC	30	2.1666874E-6	44.999996	30
CGCTTAT	30	2.1666874E-6	44.999996	23
CGGTCTA	380	0.0	43.223686	31
TTACGAT	65	0.0	41.538464	13
CCGCGTA	55	6.184564E-11	40.909092	42
TAGCACG	50	1.0822987E-9	40.5	1
CGTTTTT	8495	0.0	40.44438	1
ATATCGG	95	0.0	40.26316	2
TACGGGA	1415	0.0	40.229683	4
>>END_MODULE