FastQCFastQC Report
Thu 26 May 2016
SRR1547506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547506_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3505579
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163540.4665135203057755No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC66860.1907245564855335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG1550.045.0000041
ATATCGT351.212884E-745.00000437
CTCGACC351.212884E-745.00000422
GCGTAAT253.892449E-545.039
ATCGATC502.1827873E-1145.040
AATGCGT650.044.99999641
GGCGATA8750.044.228578
CACCGTA1600.043.5937538
TTACGAG2000.042.751
GCGATAC1950.042.6923079
TACGGGA13500.042.1666684
TAAGTCG750.042.032
CGTAAGT750.042.024
CGTTTTT95150.041.949551
TACCCGC650.041.5384640
CTATCGA603.6379788E-1241.25000412
CGTTCTA603.6379788E-1241.25000428
ACGCGAA556.184564E-1140.90909227
TCGAAAG1100.040.9090921
GACCGAT10850.040.645169
Produced by FastQC (version 0.11.5)