##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547506_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3505579
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.597857871695375	31.0	31.0	33.0	30.0	34.0
2	32.023333378024	31.0	31.0	34.0	30.0	34.0
3	32.07732759695331	33.0	31.0	34.0	30.0	34.0
4	35.80927858136987	37.0	35.0	37.0	35.0	37.0
5	35.7429078049589	37.0	35.0	37.0	35.0	37.0
6	35.795553601844375	37.0	35.0	37.0	35.0	37.0
7	36.12196929522912	37.0	35.0	37.0	35.0	37.0
8	36.12795803489238	37.0	35.0	37.0	35.0	37.0
9	37.98993261883415	39.0	39.0	39.0	35.0	39.0
10	37.482085270364756	39.0	37.0	39.0	35.0	39.0
11	37.139976876858285	39.0	37.0	39.0	33.0	39.0
12	36.47094559843039	38.0	35.0	39.0	33.0	39.0
13	36.18202214241927	38.0	35.0	39.0	32.0	39.0
14	37.21713531487951	39.0	35.0	41.0	32.0	41.0
15	37.42560786677465	39.0	35.0	41.0	33.0	41.0
16	37.534490878682234	39.0	35.0	41.0	33.0	41.0
17	37.481433166960436	39.0	35.0	41.0	33.0	41.0
18	37.37002731931016	39.0	35.0	41.0	33.0	41.0
19	37.33470904521051	39.0	35.0	41.0	32.0	41.0
20	37.18399499768797	39.0	35.0	41.0	32.0	41.0
21	37.040276370893366	39.0	35.0	41.0	32.0	41.0
22	36.97420939593716	39.0	35.0	41.0	32.0	41.0
23	36.92870649898348	38.0	35.0	41.0	32.0	41.0
24	36.82914691125204	38.0	35.0	41.0	32.0	41.0
25	36.73177041510119	38.0	35.0	41.0	31.0	41.0
26	36.694799632243345	38.0	35.0	41.0	31.0	41.0
27	36.64114943636986	38.0	35.0	41.0	31.0	41.0
28	36.640293657624035	38.0	35.0	41.0	31.0	41.0
29	36.6243231146695	38.0	35.0	41.0	31.0	41.0
30	36.58435625042254	38.0	35.0	41.0	31.0	41.0
31	36.512215813707236	38.0	35.0	41.0	31.0	41.0
32	36.36364891505797	38.0	35.0	41.0	31.0	41.0
33	36.27545521010937	38.0	35.0	41.0	30.0	41.0
34	36.14323482654363	38.0	35.0	41.0	30.0	41.0
35	35.97887624269771	38.0	35.0	41.0	30.0	41.0
36	35.83685148730067	38.0	35.0	41.0	29.0	41.0
37	35.775911768070266	38.0	35.0	41.0	29.0	41.0
38	35.65451755615834	38.0	35.0	41.0	29.0	41.0
39	35.59649832452784	38.0	35.0	40.0	29.0	41.0
40	35.410273452687846	38.0	35.0	40.0	27.0	41.0
41	35.374603738783236	38.0	34.0	40.0	27.0	41.0
42	35.33990676005305	38.0	34.0	40.0	27.0	41.0
43	35.24858518378847	38.0	34.0	40.0	27.0	41.0
44	35.11263303437178	37.0	34.0	40.0	27.0	41.0
45	35.01128715113823	37.0	34.0	40.0	26.0	41.0
46	34.96301067526934	37.0	34.0	40.0	26.0	41.0
47	34.90440323838088	37.0	34.0	40.0	26.0	41.0
48	34.79632037960063	37.0	34.0	40.0	26.0	41.0
49	34.69627385376282	36.0	34.0	40.0	26.0	41.0
50	34.58074885774932	36.0	34.0	40.0	25.0	41.0
51	34.209051914106055	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	10.0
12	15.0
13	29.0
14	59.0
15	117.0
16	314.0
17	681.0
18	1401.0
19	2753.0
20	4721.0
21	7340.0
22	11148.0
23	16013.0
24	23324.0
25	33464.0
26	45453.0
27	54487.0
28	59567.0
29	66166.0
30	77809.0
31	94454.0
32	119030.0
33	156148.0
34	280006.0
35	471156.0
36	227450.0
37	296226.0
38	474280.0
39	981638.0
40	312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.52198852172494	23.934876378481274	29.887074289297143	13.65606081049664
2	29.51084542667559	26.413896249378492	30.88867773340723	13.186580590538682
3	29.38384786079561	25.919826653457246	31.51644849538407	13.179876990363077
4	26.15479497110178	27.945540522692543	31.04434388727226	14.85532061893342
5	23.919272679349117	31.87131141531827	30.212726628040613	13.996689277291996
6	22.793838050718584	40.934521800820924	26.18968221797312	10.081957930487375
7	88.57549637306704	3.8227636575869495	5.941842987991428	1.659896981354578
8	89.22848408208743	3.0566990502852738	5.759362433423979	1.9554544342033087
9	85.02686717372508	5.6878478562314525	6.881430998987614	2.403853971055851
10	45.940770411963335	32.40243623093361	12.254837218045864	9.4019561390572
11	38.73785186412858	21.46683900148877	24.026016814911316	15.769292319471335
12	35.74348203249734	21.634657213544468	26.34503458629801	16.27682616766018
13	24.196174155538927	32.63634908812495	25.761992526769472	17.405484229566643
14	17.13671265146214	36.3557346732166	29.053773998532055	17.4537786767892
15	15.768636222432871	26.682810457273966	41.14307508117774	16.40547823911542
16	19.848704022930306	23.97618196594628	38.584439260960885	17.590674750162528
17	20.318269820762847	24.235368822097577	27.674372764099743	27.771988593039836
18	22.77957507162155	25.693416123270936	31.94895907352252	19.578049731584997
19	27.342587344344544	26.867573088496936	26.632576244894214	19.157263322264313
20	29.257791651536024	26.667720225389303	26.596690589486073	17.4777975335886
21	24.51383922598806	25.93514509300746	30.749299901671023	18.801715779333456
22	24.542365184182128	22.75159110663317	28.962405354436456	23.743638354748246
23	20.06005284719015	27.420320580423375	29.39354668658159	23.126079885804884
24	20.45331170685356	25.31125386134502	35.80170351317143	18.43373091862999
25	19.651874911391243	27.43001940620936	32.102400202648404	20.81570547975099
26	19.36684353711612	33.4179318166842	27.118088053357237	20.09713659284244
27	19.072084811096826	31.57897739574547	30.902997764420657	18.445940028737052
28	17.317396070663364	28.577076711150994	36.06685229458529	18.038674923600354
29	17.19553317725831	26.086646456976155	35.91361084716676	20.804209518598782
30	19.48970483905797	28.75168410125688	32.29010100756537	19.46851005211978
31	26.16041458486601	26.911331908366638	27.908941718329555	19.0193117884378
32	26.611695243496154	26.979280740784905	28.735338727211683	17.67368528850726
33	25.560599261919357	27.38760130637478	26.586677978159955	20.465121453545905
34	20.09217307611667	28.470532257296156	28.947800063841093	22.48949460274608
35	20.57876887099107	28.22951643651448	29.952341681645173	21.239373010849278
36	26.194018163618622	25.835332765286417	27.845129149849424	20.125519921245534
37	20.356466078784702	30.614143911747533	29.57751059097513	19.451879418492638
38	21.02708282996903	31.673312739493248	26.06550872195435	21.234095708583375
39	20.83869740205541	30.109034770005184	27.90854235491484	21.14372547302457
40	23.979947392427896	26.438143313843447	27.346409822742544	22.235499470986106
41	19.53643035857985	25.64007258144803	29.19429286859603	25.629204191376086
42	21.66295496407298	25.64669060374905	27.891654987663948	24.798699444514018
43	21.988065309610764	27.0153660779004	26.996995360823416	23.999573251665417
44	19.755281509844735	30.201230666888407	28.631304557677918	21.41218326558894
45	19.24957332298031	33.7771021563057	25.442815580536056	21.530508940177928
46	22.16983271522336	30.392839528077957	26.596291226071354	20.84103653062732
47	21.338129878117137	27.789703213078354	27.99292213925289	22.879244769551622
48	21.97303212964249	26.344264385426776	30.90622119769659	20.77648228723415
49	22.513285251879932	24.971709380961034	30.69803875479628	21.81696661236275
50	20.048186048581417	30.472911892728703	29.331103364094773	20.1477986945951
51	19.61327928995467	31.27560383035156	27.251589537705467	21.8595273419883
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2491.0
1	4222.0
2	5953.0
3	17591.0
4	29229.0
5	21392.0
6	13555.0
7	13497.0
8	13439.0
9	13896.5
10	14354.0
11	14210.0
12	14066.0
13	13488.5
14	12911.0
15	12366.5
16	11822.0
17	11759.5
18	11697.0
19	11638.5
20	11580.0
21	12628.5
22	13677.0
23	16618.0
24	19559.0
25	23978.5
26	33849.0
27	39300.0
28	45855.0
29	52410.0
30	64756.0
31	77102.0
32	89242.0
33	101382.0
34	111189.0
35	120996.0
36	123802.5
37	126609.0
38	144764.5
39	162920.0
40	208270.5
41	253621.0
42	278829.0
43	304037.0
44	304447.0
45	304857.0
46	304191.0
47	303525.0
48	280667.0
49	257809.0
50	242960.0
51	228111.0
52	209778.5
53	191446.0
54	182417.5
55	173389.0
56	159419.5
57	145450.0
58	135572.5
59	125695.0
60	116801.5
61	107908.0
62	92383.5
63	76859.0
64	64981.5
65	53104.0
66	44002.0
67	34900.0
68	29462.5
69	24025.0
70	19602.5
71	15180.0
72	12419.5
73	9659.0
74	7828.5
75	4565.5
76	3133.0
77	2298.5
78	1464.0
79	1145.5
80	827.0
81	625.0
82	423.0
83	342.5
84	262.0
85	238.0
86	214.0
87	146.5
88	79.0
89	58.0
90	37.0
91	64.0
92	91.0
93	54.0
94	17.0
95	12.5
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3505579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.740934878545303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.21554974718997	11.971931299485838
2	9.11198141122345	2.686382491947204
3	2.8930926462171063	1.27940670886454
4	1.2060545703094072	0.7111348752361164
5	0.7080941121303173	0.521898459739718
6	0.4345866404167697	0.3843728019281632
7	0.30863500251088105	0.3184697931276795
8	0.23384923952477848	0.27577251289856813
9	0.18070425814785154	0.23973747314699873
>10	1.4661963563551756	4.712118309505278
>50	0.4754834607685383	5.154334337040418
>100	1.638393861956317	60.07672055647022
>500	0.12406762629952228	10.436920568123368
>1k	0.002921529661684198	0.5693048469006334
>5k	1.947686441122799E-4	0.19195986718329894
>10k+	1.947686441122799E-4	0.46953509840198493
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16354	0.4665135203057755	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6686	0.1907245564855335	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.7051916388134456E-5	0.0	0.0	0.024732005754256287	0.0
2	5.7051916388134456E-5	0.0	0.0	0.08614839374608303	0.0
3	5.7051916388134456E-5	0.0	0.0	0.12685493608901696	0.0
4	5.7051916388134456E-5	0.0	0.0	0.23648019342881732	0.0
5	5.7051916388134456E-5	0.0	0.0	0.415822892594918	0.0
6	5.7051916388134456E-5	0.0	0.0	0.6001005825285923	0.0
7	5.7051916388134456E-5	0.0	0.0	0.6915262785405778	0.0
8	5.7051916388134456E-5	0.0	0.0	0.901677012556271	0.0
9	5.7051916388134456E-5	0.0	0.0	0.958557773195241	0.0
10	8.557787458220169E-5	0.0	0.0	1.0763414545785446	0.0
11	8.557787458220169E-5	0.0	0.0	1.250463903395131	0.0
12	8.557787458220169E-5	0.0	0.0	1.3847926405309936	0.0
13	8.557787458220169E-5	0.0	0.0	1.439248694723468	0.0
14	8.557787458220169E-5	0.0	0.0	1.460899896992765	0.0
15	8.557787458220169E-5	0.0	0.0	1.5010359201718175	0.0
16	8.557787458220169E-5	0.0	0.0	1.5874125215834531	0.0
17	1.1410383277626891E-4	0.0	0.0	1.7014022505269457	0.0
18	1.1410383277626891E-4	0.0	0.0	1.844916346201298	0.0
19	1.1410383277626891E-4	0.0	0.0	1.920938024788487	0.0
20	1.1410383277626891E-4	0.0	0.0	1.9979010599960805	0.0
21	1.1410383277626891E-4	0.0	0.0	2.1077259990432395	0.0
22	1.1410383277626891E-4	5.7051916388134456E-5	0.0	2.221601624153956	0.0
23	1.1410383277626891E-4	5.7051916388134456E-5	0.0	2.335733982888419	0.0
24	1.1410383277626891E-4	5.7051916388134456E-5	0.0	2.427530516356927	0.0
25	1.1410383277626891E-4	5.7051916388134456E-5	0.0	2.5071464656765685	0.0
26	1.1410383277626891E-4	5.7051916388134456E-5	0.0	2.5892726993172883	0.0
27	1.4262979097033615E-4	5.7051916388134456E-5	0.0	2.6771611765132093	0.0
28	1.4262979097033615E-4	5.7051916388134456E-5	0.0	2.763052836635546	0.0
29	1.4262979097033615E-4	5.7051916388134456E-5	0.0	2.867999836831519	0.0
30	1.4262979097033615E-4	5.7051916388134456E-5	0.0	2.98295944835361	0.0
31	1.4262979097033615E-4	5.7051916388134456E-5	0.0	3.0931552248572918	0.0
32	1.4262979097033615E-4	5.7051916388134456E-5	0.0	3.1873194128559077	0.0
33	1.4262979097033615E-4	5.7051916388134456E-5	0.0	3.283337788137138	0.0
34	1.996817073584706E-4	5.7051916388134456E-5	0.0	3.3998378014017083	0.0
35	1.996817073584706E-4	5.7051916388134456E-5	0.0	3.5308290014288652	0.0
36	2.2820766555253782E-4	5.7051916388134456E-5	0.0	3.6412529855980993	0.0
37	2.2820766555253782E-4	5.7051916388134456E-5	0.0	3.759749815936255	0.0
38	2.567336237466051E-4	5.7051916388134456E-5	0.0	3.889542925719261	0.0
39	2.567336237466051E-4	5.7051916388134456E-5	0.0	4.080552741786735	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	155	0.0	45.000004	1
ATATCGT	35	1.212884E-7	45.000004	37
CTCGACC	35	1.212884E-7	45.000004	22
GCGTAAT	25	3.892449E-5	45.0	39
ATCGATC	50	2.1827873E-11	45.0	40
AATGCGT	65	0.0	44.999996	41
GGCGATA	875	0.0	44.22857	8
CACCGTA	160	0.0	43.59375	38
TTACGAG	200	0.0	42.75	1
GCGATAC	195	0.0	42.692307	9
TACGGGA	1350	0.0	42.166668	4
TAAGTCG	75	0.0	42.0	32
CGTAAGT	75	0.0	42.0	24
CGTTTTT	9515	0.0	41.94955	1
TACCCGC	65	0.0	41.53846	40
CTATCGA	60	3.6379788E-12	41.250004	12
CGTTCTA	60	3.6379788E-12	41.250004	28
ACGCGAA	55	6.184564E-11	40.909092	27
TCGAAAG	110	0.0	40.909092	1
GACCGAT	1085	0.0	40.64516	9
>>END_MODULE