FastQCFastQC Report
Thu 26 May 2016
SRR1547505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547505_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3112756
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210600.6765708587502522No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC47740.15336891166541805No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG351.2128294E-745.00000437
TAGCGAT351.2128294E-745.00000415
CGTTACC302.166702E-645.00000417
TCGTCTA551.8189894E-1245.00000410
TACCTCG700.045.00000434
ATACCCG650.045.00000432
GCGTACA302.166702E-645.00000436
TACGTCC750.045.00000428
ACGCGAG207.0351455E-445.01
CTCCGTA453.8562575E-1045.045
TCCGATT406.8212103E-945.043
GCGCGAT502.1827873E-1145.09
CGCGACA406.8212103E-945.029
ATATCCG406.8212103E-945.015
TCGCTAA406.8212103E-945.026
GCGATCG207.0351455E-445.09
ATGCGTA253.8923474E-545.044
GTACGTA502.1827873E-1145.014
GTTAGCG4400.044.488641
TACGGAC850.042.3529430