FastQCFastQC Report
Thu 26 May 2016
SRR1547504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547504_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3171290
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139030.4384020382872585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC302.1667147E-645.00000433
CGGTCGA207.035166E-445.042
TACGGGA13150.043.117874
CGCATCG750.042.00000421
ATAACGG1600.040.781252
CGTTTTT71900.040.4624441
GCGTAAG1800.040.01
CGTAAGG4800.039.8437542
CGGTCTA3850.039.7402631
TACGACG403.460791E-739.3751
TAGGGAC35500.039.3591545
GGCGATA6950.039.172668
CACGACG3850.039.15584626
GCTATCG356.2517665E-638.571431
CGACATA700.038.5714311
TAACGGC700.038.5714313
ACGGGAT17250.038.3478285
GGGCGAT35050.038.1312377
ACGGGAC9500.037.6578985
CGTAATG301.1403746E-437.50000423
Produced by FastQC (version 0.11.5)