##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547504_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3171290
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64909737047069	31.0	31.0	33.0	30.0	34.0
2	32.09529875854936	33.0	31.0	34.0	30.0	34.0
3	32.16076801553942	33.0	31.0	34.0	30.0	34.0
4	35.882670774353656	37.0	35.0	37.0	35.0	37.0
5	35.826047444415366	37.0	35.0	37.0	35.0	37.0
6	35.87947239136125	37.0	35.0	37.0	35.0	37.0
7	36.17601922246152	37.0	35.0	37.0	35.0	37.0
8	36.179044805110856	37.0	35.0	37.0	35.0	37.0
9	38.01311516764471	39.0	39.0	39.0	35.0	39.0
10	37.52923384490223	39.0	37.0	39.0	35.0	39.0
11	37.23538402353616	39.0	37.0	39.0	34.0	39.0
12	36.814724607336444	39.0	35.0	39.0	33.0	39.0
13	36.629395608727044	39.0	35.0	39.0	33.0	39.0
14	37.73119708383655	40.0	36.0	41.0	33.0	41.0
15	37.874645648931505	40.0	36.0	41.0	33.0	41.0
16	37.95409596725623	40.0	36.0	41.0	33.0	41.0
17	37.87877456807797	40.0	36.0	41.0	33.0	41.0
18	37.75396825897348	39.0	36.0	41.0	33.0	41.0
19	37.74897029284613	40.0	36.0	41.0	33.0	41.0
20	37.6451831904367	40.0	35.0	41.0	33.0	41.0
21	37.53866533808009	39.0	35.0	41.0	33.0	41.0
22	37.47803322937984	39.0	35.0	41.0	33.0	41.0
23	37.39408284956595	39.0	35.0	41.0	32.0	41.0
24	37.311720782394545	39.0	35.0	41.0	32.0	41.0
25	37.21133797287539	39.0	35.0	41.0	32.0	41.0
26	37.19965093069382	39.0	35.0	41.0	32.0	41.0
27	37.153668381005836	39.0	35.0	41.0	32.0	41.0
28	37.14283020474318	39.0	35.0	41.0	32.0	41.0
29	37.105427129023205	39.0	35.0	41.0	32.0	41.0
30	37.03936694531247	39.0	35.0	41.0	32.0	41.0
31	37.00231104692412	39.0	35.0	41.0	32.0	41.0
32	36.89965849859205	39.0	35.0	41.0	31.0	41.0
33	36.81840292120872	39.0	35.0	41.0	31.0	41.0
34	36.71073317167462	39.0	35.0	41.0	31.0	41.0
35	36.53783917585588	39.0	35.0	41.0	30.0	41.0
36	36.430848645188554	39.0	35.0	41.0	30.0	41.0
37	36.38009012105484	39.0	35.0	41.0	30.0	41.0
38	36.24391651346928	39.0	35.0	41.0	30.0	41.0
39	36.15473198603723	39.0	35.0	41.0	30.0	41.0
40	35.997651428913784	39.0	35.0	41.0	29.0	41.0
41	35.925115016286746	38.0	35.0	41.0	29.0	41.0
42	35.89307663442952	38.0	35.0	41.0	29.0	41.0
43	35.80029956263855	38.0	35.0	41.0	28.0	41.0
44	35.66966975584068	38.0	35.0	40.0	28.0	41.0
45	35.57516562660621	38.0	35.0	40.0	28.0	41.0
46	35.51816768570519	38.0	34.0	40.0	27.0	41.0
47	35.44165245058005	38.0	34.0	40.0	27.0	41.0
48	35.303532316502114	38.0	34.0	40.0	27.0	41.0
49	35.206890571344786	38.0	34.0	40.0	27.0	41.0
50	35.12730213887724	38.0	34.0	40.0	26.0	41.0
51	34.760084382065344	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	10.0
11	17.0
12	22.0
13	40.0
14	49.0
15	102.0
16	245.0
17	530.0
18	1037.0
19	2082.0
20	3464.0
21	5353.0
22	8203.0
23	11891.0
24	17320.0
25	25381.0
26	35054.0
27	41485.0
28	46754.0
29	52986.0
30	62893.0
31	77269.0
32	97447.0
33	127809.0
34	216826.0
35	324603.0
36	218505.0
37	298393.0
38	485555.0
39	1009664.0
40	297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.35371410372435	23.603643943001114	29.73389377824166	13.308748175032873
2	30.34456010014852	25.403510874123782	31.22265702600519	13.02927199972251
3	29.406172251670455	25.234589078892185	31.993983520901587	13.36525514853577
4	27.170425915006195	27.747635820123673	31.023778966918826	14.058159297951306
5	24.0089048935922	31.794758599812695	29.288932894815673	14.907403611779433
6	22.530862834997745	40.830955226422056	26.0027622828565	10.635419655723696
7	89.36514793664408	3.4118607885119308	5.577477934846703	1.645513339997288
8	89.85488555130561	2.907302706469607	5.495870765524439	1.7419409767003333
9	84.35772193649903	6.11634382223007	6.844091836445106	2.6818424048257965
10	40.27730040456723	38.422597744135665	12.092460796710487	9.20764105458662
11	32.55148535769356	22.38243743082468	28.276127380340494	16.78994983114127
12	31.457577200445247	21.595407547086516	29.42619564908917	17.520819603379067
13	25.352963620482516	26.504198606876066	29.761989600446505	18.38084817219491
14	19.04817913215127	31.383853258453186	30.974808358743605	18.59315925065194
15	18.535107164592326	27.96187040604927	36.54304715115931	16.959975278199092
16	24.005310141929627	26.517505494609452	33.57718783208095	15.899996531379976
17	23.413595098524574	25.72010128370474	28.567617594102085	22.2986860236686
18	24.06251083943758	26.740159367323706	30.848329859457824	18.348999933780892
19	26.68589123038259	27.119184937359876	27.7726729501244	18.422250882133138
20	27.8797902430872	27.66959186955466	27.39686373683895	17.05375415051919
21	26.53494319346386	25.247265308439154	31.098953422739644	17.118838075357345
22	25.120250749694918	22.503050808976788	30.91376064629851	21.46293779502978
23	21.095074874893182	25.694401962608275	31.834426999738273	21.376096162760263
24	21.345950701449567	26.19069842240855	32.83171832282762	19.631632553314265
25	21.468046126339754	27.955059297635977	30.886137817733477	19.69075675829079
26	21.11758937214824	32.15063901440738	26.900378079582755	19.831393533861615
27	19.835398213345357	30.88818745683933	30.785358639544157	18.491055690271153
28	18.350671177974892	29.201649801815666	33.08338247211702	19.364296548092415
29	19.61075776734389	26.869665025904283	32.97837788407872	20.541199322673105
30	21.297736883098047	27.101873370142748	32.004168650612215	19.596221096146994
31	24.87788880865515	26.221569140633623	29.162927389169706	19.737614661541517
32	25.136773994179023	27.04565019282374	29.03045763711297	18.787118175884263
33	24.394489308767096	27.429563363804633	28.485095970409517	19.69085135701875
34	21.771897240555106	27.752271157793835	29.946204856698692	20.52962674495237
35	21.276893630036987	27.57811489961498	30.42405456454629	20.72093690580174
36	24.813214811638165	26.378981423963122	29.498279879796552	19.309523884602164
37	21.988023801039954	29.26793197720801	29.20404630292405	19.539997918827982
38	21.55791491790407	29.561282632619534	27.702102299064418	21.178700150411977
39	20.8707182250756	28.792352638831513	29.21202412898221	21.12490500711067
40	22.72936249917226	27.449618294132673	29.06615289046413	20.754866316230935
41	20.550154668920218	26.445232066446145	29.73263246186883	23.271980802764805
42	21.757329036448887	26.57729189068171	28.863553948077914	22.80182512479149
43	21.759914735013197	26.620964970091038	29.31255104389696	22.306569250998805
44	21.341189232142124	28.210223599859997	29.561787159168667	20.886800008829216
45	20.449722352733428	29.959511744432078	28.04609480684516	21.54467109598933
46	21.985658832840894	28.724367686335846	28.19877715377654	21.09119632704672
47	21.369537317621536	28.215363464079285	29.106546547304095	21.308552670995084
48	21.696596653097007	27.574236351768523	30.120298049058903	20.608868946075575
49	21.263271413210397	26.448164627012982	31.036959723014924	21.251604236761697
50	20.683444276619294	29.273828631251003	29.603568264018744	20.43915882811096
51	20.692904149415536	29.727082669828363	28.027458857436567	21.552554323319534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2192.0
1	3716.5
2	5241.0
3	15279.5
4	25318.0
5	18395.5
6	11473.0
7	11074.5
8	10676.0
9	10827.0
10	10978.0
11	10725.0
12	10472.0
13	10758.0
14	11044.0
15	10475.0
16	9906.0
17	9935.5
18	9965.0
19	10253.5
20	10542.0
21	13087.5
22	15633.0
23	19711.5
24	23790.0
25	28750.5
26	38784.0
27	43857.0
28	52967.0
29	62077.0
30	71833.0
31	81589.0
32	91063.0
33	100537.0
34	109738.0
35	118939.0
36	128738.0
37	138537.0
38	144793.5
39	151050.0
40	176919.5
41	202789.0
42	221638.0
43	240487.0
44	245492.5
45	250498.0
46	246780.0
47	243062.0
48	237939.0
49	232816.0
50	218985.5
51	205155.0
52	192351.0
53	179547.0
54	169925.0
55	160303.0
56	150269.5
57	140236.0
58	126597.0
59	112958.0
60	104504.5
61	96051.0
62	84313.5
63	72576.0
64	62232.5
65	51889.0
66	43719.0
67	35549.0
68	29826.5
69	24104.0
70	19062.0
71	14020.0
72	11434.0
73	8848.0
74	7154.5
75	4145.5
76	2830.0
77	2457.0
78	2084.0
79	1591.0
80	1098.0
81	952.5
82	807.0
83	545.0
84	283.0
85	218.0
86	153.0
87	111.0
88	69.0
89	56.5
90	44.0
91	33.0
92	22.0
93	17.5
94	13.0
95	11.0
96	9.0
97	5.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3171290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.196579357600108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.02133139366018	13.284650029334344
2	7.396867489219142	2.396079025735802
3	2.142457918471998	1.0410146909055136
4	1.0444832827351667	0.676682255060272
5	0.6373137688445752	0.5161151516391188
6	0.43624936335580133	0.4239448459976858
7	0.32277529327304233	0.36594989565186353
8	0.2445738200096511	0.3169007427662176
9	0.19609598274178344	0.2858475731565489
>10	2.3546117052015045	9.25444168442035
>50	1.2237294613217047	14.680161882312662
>100	1.9742182368607197	55.607161207443646
>500	0.003920210596060379	0.42763210358851417
>1k	0.0011760631788181137	0.2820403569621648
>5k	0.0	0.0
>10k+	1.9601052980301896E-4	0.4413785550253238
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13903	0.4384020382872585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2613163728325067E-4	0.0	0.0	0.021473911247473425	0.0
2	1.2613163728325067E-4	0.0	0.0	0.07016072323880818	0.0
3	1.2613163728325067E-4	0.0	0.0	0.10200896165282898	0.0
4	1.2613163728325067E-4	0.0	0.0	0.1682596041358564	0.0
5	1.2613163728325067E-4	0.0	0.0	0.2880846595549445	0.0
6	1.2613163728325067E-4	0.0	0.0	0.4169596599491059	0.0
7	1.2613163728325067E-4	0.0	0.0	0.4856383364498359	0.0
8	1.2613163728325067E-4	0.0	0.0	0.6404018553963844	0.0
9	1.2613163728325067E-4	0.0	0.0	0.6954898479798441	0.0
10	1.2613163728325067E-4	0.0	0.0	0.7988862576427889	0.0
11	1.2613163728325067E-4	0.0	0.0	0.9430547190575445	0.0
12	1.2613163728325067E-4	0.0	0.0	1.0601994771843635	0.0
13	1.2613163728325067E-4	0.0	0.0	1.1056068666063337	0.0
14	1.5766454660406332E-4	0.0	0.0	1.1261347905741828	0.0
15	1.5766454660406332E-4	0.0	0.0	1.158582154265299	0.0
16	1.5766454660406332E-4	0.0	0.0	1.2257787840279508	0.0
17	1.5766454660406332E-4	0.0	0.0	1.3110122379221074	0.0
18	1.5766454660406332E-4	0.0	0.0	1.4090165200911933	0.0
19	1.5766454660406332E-4	0.0	0.0	1.4795871711511719	0.0
20	1.5766454660406332E-4	0.0	0.0	1.55245972459157	0.0
21	1.5766454660406332E-4	0.0	0.0	1.6423600490652068	0.0
22	1.5766454660406332E-4	0.0	0.0	1.7477745649246836	0.0
23	1.5766454660406332E-4	0.0	0.0	1.8484591443860385	0.0
24	1.5766454660406332E-4	0.0	0.0	1.928174339149053	0.0
25	1.5766454660406332E-4	0.0	0.0	2.002055945687717	0.0
26	1.5766454660406332E-4	0.0	0.0	2.075149229493361	0.0
27	1.5766454660406332E-4	0.0	0.0	2.161675532669671	0.0
28	1.5766454660406332E-4	0.0	0.0	2.2452062094605036	0.0
29	1.5766454660406332E-4	0.0	0.0	2.3448502029142713	0.0
30	1.5766454660406332E-4	0.0	0.0	2.4539225362549626	0.0
31	2.2073036524568866E-4	0.0	0.0	2.5483005338521547	0.0
32	2.2073036524568866E-4	0.0	0.0	2.6342277117513695	0.0
33	2.2073036524568866E-4	0.0	0.0	2.7220153313005118	0.0
34	2.2073036524568866E-4	0.0	0.0	2.8185375667315196	0.0
35	2.2073036524568866E-4	0.0	0.0	2.9249926685985828	0.0
36	2.2073036524568866E-4	0.0	0.0	3.0248573924175965	0.0
37	2.2073036524568866E-4	0.0	0.0	3.1362000952293863	0.0
38	2.5226327456650134E-4	0.0	0.0	3.2655480892633597	0.0
39	2.83796183887314E-4	0.0	0.0	3.4902831339927918	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTTC	30	2.1667147E-6	45.000004	33
CGGTCGA	20	7.035166E-4	45.0	42
TACGGGA	1315	0.0	43.11787	4
CGCATCG	75	0.0	42.000004	21
ATAACGG	160	0.0	40.78125	2
CGTTTTT	7190	0.0	40.462444	1
GCGTAAG	180	0.0	40.0	1
CGTAAGG	480	0.0	39.843754	2
CGGTCTA	385	0.0	39.74026	31
TACGACG	40	3.460791E-7	39.375	1
TAGGGAC	3550	0.0	39.359154	5
GGCGATA	695	0.0	39.17266	8
CACGACG	385	0.0	39.155846	26
GCTATCG	35	6.2517665E-6	38.57143	1
CGACATA	70	0.0	38.57143	11
TAACGGC	70	0.0	38.57143	13
ACGGGAT	1725	0.0	38.347828	5
GGGCGAT	3505	0.0	38.131237	7
ACGGGAC	950	0.0	37.657898	5
CGTAATG	30	1.1403746E-4	37.500004	23
>>END_MODULE