FastQCFastQC Report
Thu 26 May 2016
SRR1547503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547503_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4477049
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234610.5240282159073979No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC77680.17350714723024027No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG207.0354773E-445.00000418
CCGTCGA207.0354773E-445.00000421
GCTCGAC302.1669166E-645.00000426
TAGTCGA650.044.9999961
TAGACCG2050.042.804881
TCGCGCG800.042.1875041
GTCGTTA700.041.78571316
CGACTAG603.6379788E-1241.25000419
CGAGGGT8700.041.120694
CTTACGC556.184564E-1140.90909236
CGTTTTT112150.040.626391
CGACGGT4050.040.55555728
TACGGGA15150.039.801984
GCGATAT1700.039.7058839
CTTTACG850.039.7058831
CGGGTAC3650.039.4520576
AGGGCGA35600.039.2485966
TAACGCC2600.038.94230712
TAGGGTA23800.038.855045
TAGGGAC38650.038.7128075
Produced by FastQC (version 0.11.5)