##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547503_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4477049
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59058723726276	31.0	31.0	33.0	30.0	34.0
2	32.028323790961416	33.0	31.0	34.0	30.0	34.0
3	32.08872920533146	33.0	31.0	34.0	30.0	34.0
4	35.80866838848536	37.0	35.0	37.0	35.0	37.0
5	35.76066980727707	37.0	35.0	37.0	35.0	37.0
6	35.806643170534876	37.0	35.0	37.0	35.0	37.0
7	36.145613326992844	37.0	35.0	37.0	35.0	37.0
8	36.16058501928391	37.0	35.0	37.0	35.0	37.0
9	38.07249842474362	39.0	39.0	39.0	35.0	39.0
10	37.409943246098045	39.0	37.0	39.0	35.0	39.0
11	37.147789983982754	39.0	37.0	39.0	33.0	39.0
12	36.631233654132444	39.0	35.0	39.0	33.0	39.0
13	36.3883294554069	39.0	35.0	39.0	32.0	39.0
14	37.52290738832655	40.0	35.0	41.0	33.0	41.0
15	37.65021289693278	40.0	35.0	41.0	33.0	41.0
16	37.74458845547592	40.0	35.0	41.0	33.0	41.0
17	37.66371286085991	40.0	35.0	41.0	33.0	41.0
18	37.56310172169212	39.0	36.0	41.0	33.0	41.0
19	37.543863379650304	39.0	36.0	41.0	33.0	41.0
20	37.43239553554138	39.0	35.0	41.0	33.0	41.0
21	37.31457149564367	39.0	35.0	41.0	32.0	41.0
22	37.233813835854825	39.0	35.0	41.0	32.0	41.0
23	37.15543095463105	39.0	35.0	41.0	32.0	41.0
24	37.04265845649668	39.0	35.0	41.0	32.0	41.0
25	36.94242479812037	39.0	35.0	41.0	32.0	41.0
26	36.90297738532681	39.0	35.0	41.0	32.0	41.0
27	36.8538702614155	39.0	35.0	41.0	32.0	41.0
28	36.88308481770023	39.0	35.0	41.0	32.0	41.0
29	36.85634845631576	39.0	35.0	41.0	31.0	41.0
30	36.80742024489792	38.0	35.0	41.0	31.0	41.0
31	36.725742112717555	39.0	35.0	41.0	31.0	41.0
32	36.615099812398746	39.0	35.0	41.0	31.0	41.0
33	36.53526753895255	39.0	35.0	41.0	31.0	41.0
34	36.448893233020236	39.0	35.0	41.0	30.0	41.0
35	36.2943322711009	38.0	35.0	41.0	30.0	41.0
36	36.176160904202746	38.0	35.0	41.0	30.0	41.0
37	36.12682818526221	38.0	35.0	41.0	30.0	41.0
38	36.02387755863293	38.0	35.0	41.0	30.0	41.0
39	35.97235857816164	38.0	35.0	41.0	30.0	41.0
40	35.834305141623425	38.0	35.0	41.0	29.0	41.0
41	35.79680208994809	38.0	35.0	41.0	29.0	41.0
42	35.7716790680647	38.0	35.0	40.0	29.0	41.0
43	35.66382074442339	38.0	35.0	40.0	29.0	41.0
44	35.53927151567919	38.0	35.0	40.0	28.0	41.0
45	35.42553655320726	38.0	34.0	40.0	28.0	41.0
46	35.38503331100464	38.0	34.0	40.0	28.0	41.0
47	35.32272217704117	38.0	34.0	40.0	27.0	41.0
48	35.188971798164374	37.0	34.0	40.0	27.0	41.0
49	35.1148368043325	37.0	34.0	40.0	27.0	41.0
50	34.9892978611581	37.0	34.0	40.0	27.0	41.0
51	34.611415912579915	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	4.0
11	7.0
12	7.0
13	13.0
14	30.0
15	97.0
16	237.0
17	634.0
18	1322.0
19	2643.0
20	4681.0
21	7624.0
22	12048.0
23	17807.0
24	26450.0
25	38151.0
26	51142.0
27	61546.0
28	69328.0
29	78834.0
30	94333.0
31	115828.0
32	145678.0
33	189499.0
34	332244.0
35	555980.0
36	293837.0
37	387149.0
38	632299.0
39	1357186.0
40	407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.91530849896885	23.917674343077326	30.850745658580013	13.316271499373805
2	28.83935377968836	26.03710613844075	31.62312943190928	13.500410649961617
3	28.678868602956992	25.484442989120737	32.29066735700235	13.546021050919926
4	24.347354697257053	27.313013549773522	32.9036380883926	15.435993664576822
5	23.33954799243877	32.27762305036197	30.33643366422838	14.046395292970884
6	20.49361085840249	41.780489782443745	27.044019397598728	10.681879961555033
7	90.98589271638528	3.0518540225939006	4.844575076127154	1.1176781848936654
8	92.51209892945107	1.9300213153798405	4.3149404887013745	1.2429392664677112
9	89.2523847739884	3.2226361605602265	5.596253246278966	1.928725819172406
10	41.29820781501387	27.881356670431796	14.807342961848308	16.013092552706034
11	33.113017078883885	28.917530274964605	22.694725923258822	15.274726722892693
12	32.78695408515743	23.39719757366962	26.299689818002886	17.516158523170063
13	21.641241809057707	31.9935073303866	27.513770789642912	18.851480070912782
14	17.36320062612672	34.98887325110804	29.980350896315855	17.667575226449387
15	15.89495670027288	27.41278909388751	40.3634626290666	16.328791576773007
16	19.768110646097462	23.512407391565294	38.864640525488994	17.854841436848247
17	19.348526227879123	23.426413246761427	29.07368224024352	28.15137828511593
18	21.544481644047227	26.291224420371545	32.45088450003563	19.7134094355456
19	26.032951616120354	27.185831560029833	27.209932256716424	19.571284567133397
20	27.276382277701227	27.310243868226593	26.981701562792814	18.431672291279366
21	23.94181971204693	26.592762330722756	30.50902502965681	18.9563929275735
22	23.906439263899053	23.02009649659854	29.59570020341524	23.477764036087166
23	20.416506497918608	28.654343519581758	28.339001873778912	22.590148108720722
24	20.164264451874438	26.052361723090367	35.47618085037711	18.307192974658086
25	19.216519631569813	27.547275001904154	33.14221041583418	20.09399495069185
26	19.694133345424632	32.60346268267334	26.799527992657662	20.902875979244364
27	18.275408645292917	32.50462525650267	30.042713403404786	19.17725269479963
28	16.762715797839157	29.530411661788825	35.590139844348364	18.116732696023654
29	17.498244937681047	27.653192984932705	34.61927711758348	20.22928495980276
30	19.550556627814437	29.271558117858437	32.500269708908704	18.677615545418423
31	25.31547007861652	27.320496157178535	27.786673766581515	19.577359997623432
32	25.67182088022713	27.64229294787705	28.651350476619758	18.034535695276062
33	24.280569634149636	27.804699032778064	27.39328964235147	20.521441690720827
34	19.713543452394646	28.42944091074277	29.73420661690323	22.12280901995935
35	20.015260051877924	27.67485904219498	30.937074845506494	21.3728060604206
36	26.14997066147813	26.25204682816739	28.884785491514613	18.713197018839868
37	20.391266658015134	30.283519344997117	30.089105569315862	19.236108427671887
38	20.583446819545642	31.063787776278527	26.684094813346913	21.668670590828913
39	20.304714109673583	29.63740177960974	28.977748512468814	21.080135598247864
40	22.62664536394397	26.88998936576303	27.638495803820778	22.844869466472222
41	18.399307222235002	26.073357696107415	30.067640537327158	25.45969454433043
42	21.527818882482634	27.267269131966167	27.832708554228464	23.37220343132273
43	21.981220218943324	26.361985316667297	28.42443761504509	23.23235684934429
44	20.678129723395923	29.609995333980038	29.03472800945444	20.677146933169592
45	19.698131514754476	32.26091561651436	26.481617690581455	21.559335178149713
46	22.532766561187962	30.002865726955413	27.843117196170958	19.621250515685666
47	21.869829881245437	28.683492184249044	28.275433215048572	21.171244719456947
48	22.508732872925894	26.532901471482667	30.279833881648383	20.67853177394306
49	21.49377860282521	25.909187056027307	30.609291968883966	21.987742372263515
50	20.31898690409687	30.288902355100422	28.566339122042223	20.825771618760484
51	19.500769368394224	30.23933845709529	27.90740061142954	22.35249156308095
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4480.0
1	6222.0
2	7964.0
3	23226.0
4	38488.0
5	27394.5
6	16301.0
7	16205.0
8	16109.0
9	16351.0
10	16593.0
11	16626.5
12	16660.0
13	16312.0
14	15964.0
15	15254.0
16	14544.0
17	14417.0
18	14290.0
19	15352.5
20	16415.0
21	18075.5
22	19736.0
23	23894.0
24	28052.0
25	33008.5
26	41961.5
27	45958.0
28	60046.5
29	74135.0
30	89051.5
31	103968.0
32	110352.5
33	116737.0
34	136489.5
35	156242.0
36	159419.5
37	162597.0
38	191420.5
39	220244.0
40	260758.0
41	301272.0
42	347641.0
43	394010.0
44	410109.5
45	426209.0
46	406724.5
47	387240.0
48	368178.5
49	349117.0
50	331624.0
51	314131.0
52	294251.0
53	274371.0
54	245422.0
55	216473.0
56	200966.0
57	185459.0
58	170653.5
59	155848.0
60	137221.5
61	118595.0
62	101615.5
63	84636.0
64	69696.0
65	54756.0
66	41507.0
67	28258.0
68	23342.0
69	18426.0
70	13723.5
71	9021.0
72	8095.0
73	7169.0
74	5499.0
75	3057.0
76	2285.0
77	1808.0
78	1331.0
79	931.0
80	531.0
81	433.0
82	335.0
83	231.5
84	128.0
85	101.5
86	75.0
87	67.0
88	59.0
89	40.5
90	22.0
91	14.5
92	7.0
93	6.0
94	5.0
95	6.5
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4477049.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.657644336982138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.22483010992765	11.75907026746135
2	9.296503575606781	2.7252968597745384
3	2.9492065501640705	1.2968526206580904
4	1.349687614263907	0.7913296406364116
5	0.7612333104088976	0.5578943560718572
6	0.4809064995721999	0.4229373858043416
7	0.3219041847900348	0.33028499358669533
8	0.26363688937545593	0.30914366068589905
9	0.19577233430478055	0.25826051225342167
>10	1.6725471694433045	5.5134385007413425
>50	0.5935215378070178	6.406494173877928
>100	1.840951472070836	63.94998573297648
>500	0.04407707757040172	3.876644379806571
>1k	0.004914517359766045	1.099370432647238
>5k	1.5357866749268891E-4	0.17486556342125323
>10k+	1.5357866749268891E-4	0.5281309195965528
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23461	0.5240282159073979	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7768	0.17350714723024027	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.467228301499492E-5	0.0	0.0	0.032231052195318834	0.0
2	4.467228301499492E-5	0.0	0.0	0.11304321216944464	0.0
3	4.467228301499492E-5	0.0	0.0	0.15983742862765182	0.0
4	4.467228301499492E-5	0.0	0.0	0.28862762055988217	0.0
5	4.467228301499492E-5	0.0	0.0	0.4908813819102717	0.0
6	4.467228301499492E-5	0.0	0.0	0.7200725299187032	0.0
7	4.467228301499492E-5	0.0	0.0	0.8278890849753934	0.0
8	4.467228301499492E-5	0.0	0.0	1.0755298858690177	0.0
9	4.467228301499492E-5	0.0	0.0	1.1451293028063798	0.0
10	4.467228301499492E-5	0.0	0.0	1.305033739858554	0.0
11	4.467228301499492E-5	0.0	0.0	1.524598010877254	0.0
12	4.467228301499492E-5	0.0	0.0	1.680347925609034	0.0
13	4.467228301499492E-5	0.0	0.0	1.7447653577166566	0.0
14	4.467228301499492E-5	0.0	0.0	1.767950272601439	0.0
15	4.467228301499492E-5	0.0	0.0	1.8077086044847845	0.0
16	4.467228301499492E-5	0.0	0.0	1.9106558806928402	0.0
17	4.467228301499492E-5	0.0	0.0	2.0399151315967283	0.0
18	4.467228301499492E-5	0.0	0.0	2.197742307488705	0.0
19	4.467228301499492E-5	0.0	0.0	2.290146924905222	0.0
20	4.467228301499492E-5	0.0	0.0	2.3863263502365064	0.0
21	4.467228301499492E-5	0.0	0.0	2.5311315556296123	0.0
22	4.467228301499492E-5	0.0	0.0	2.6765621729849283	0.0
23	4.467228301499492E-5	0.0	0.0	2.835550828235295	0.0
24	4.467228301499492E-5	0.0	0.0	2.9524805290270444	0.0
25	4.467228301499492E-5	0.0	0.0	3.051340291339228	0.0
26	4.467228301499492E-5	0.0	0.0	3.1471400022648846	6.700842452249239E-5
27	4.467228301499492E-5	0.0	0.0	3.2431184023226014	6.700842452249239E-5
28	4.467228301499492E-5	0.0	0.0	3.3509796296623064	6.700842452249239E-5
29	4.467228301499492E-5	0.0	0.0	3.469450524218073	6.700842452249239E-5
30	4.467228301499492E-5	0.0	0.0	3.613630317648969	6.700842452249239E-5
31	6.700842452249239E-5	0.0	0.0	3.7426438709962744	6.700842452249239E-5
32	6.700842452249239E-5	0.0	0.0	3.863080346004701	6.700842452249239E-5
33	6.700842452249239E-5	0.0	0.0	3.995310303729086	6.700842452249239E-5
34	6.700842452249239E-5	0.0	0.0	4.126691488076186	6.700842452249239E-5
35	6.700842452249239E-5	0.0	0.0	4.280297133223246	6.700842452249239E-5
36	6.700842452249239E-5	0.0	0.0	4.417351697513251	6.700842452249239E-5
37	6.700842452249239E-5	0.0	0.0	4.561732516217714	6.700842452249239E-5
38	6.700842452249239E-5	0.0	0.0	4.71323856406307	6.700842452249239E-5
39	6.700842452249239E-5	0.0	0.0	4.8847131224161275	6.700842452249239E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	20	7.0354773E-4	45.000004	18
CCGTCGA	20	7.0354773E-4	45.000004	21
GCTCGAC	30	2.1669166E-6	45.000004	26
TAGTCGA	65	0.0	44.999996	1
TAGACCG	205	0.0	42.80488	1
TCGCGCG	80	0.0	42.187504	1
GTCGTTA	70	0.0	41.785713	16
CGACTAG	60	3.6379788E-12	41.250004	19
CGAGGGT	870	0.0	41.12069	4
CTTACGC	55	6.184564E-11	40.909092	36
CGTTTTT	11215	0.0	40.62639	1
CGACGGT	405	0.0	40.555557	28
TACGGGA	1515	0.0	39.80198	4
GCGATAT	170	0.0	39.705883	9
CTTTACG	85	0.0	39.705883	1
CGGGTAC	365	0.0	39.452057	6
AGGGCGA	3560	0.0	39.248596	6
TAACGCC	260	0.0	38.942307	12
TAGGGTA	2380	0.0	38.85504	5
TAGGGAC	3865	0.0	38.712807	5
>>END_MODULE