FastQCFastQC Report
Thu 26 May 2016
SRR1547502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547502_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2922982
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141030.4824867207529845No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC46060.15757880137476044No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG207.0350745E-445.0000042
ACTACGT207.0350745E-445.00000445
CGAATTA207.0350745E-445.0000042
ACGACGT207.0350745E-445.00000427
ATATGCG453.8562575E-1045.01
ATCGTAC253.8922895E-545.012
ATATCGG502.1827873E-1145.02
CGACTCG253.8922895E-545.021
AGTCGAC351.212793E-745.041
TAATCGC351.212793E-745.024
CCTTACG600.044.99999618
ACACCGT302.1666565E-644.99999610
CGACATA302.1666565E-644.99999618
CGTTAGT302.1666565E-644.99999619
CGAATCG302.1666565E-644.99999645
CGTAAGA302.1666565E-644.99999628
TATCGCC551.8189894E-1244.99999633
CGGTCTA4000.044.437531
TCACGAC4100.043.9024425
CCCGTAG750.042.037
Produced by FastQC (version 0.11.5)