##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547502_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2922982
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.587289281973	31.0	31.0	33.0	30.0	34.0
2	32.01491114211446	31.0	31.0	34.0	30.0	34.0
3	32.05761479201719	33.0	31.0	34.0	30.0	34.0
4	35.78447044832982	37.0	35.0	37.0	35.0	37.0
5	35.734327820013945	37.0	35.0	37.0	33.0	37.0
6	35.783055112894985	37.0	35.0	37.0	35.0	37.0
7	36.113485474765156	37.0	35.0	37.0	35.0	37.0
8	36.12435177500238	37.0	35.0	37.0	35.0	37.0
9	37.9573312459673	39.0	38.0	39.0	35.0	39.0
10	37.44142146616024	39.0	37.0	39.0	35.0	39.0
11	37.07863065869034	39.0	37.0	39.0	33.0	39.0
12	36.36947473504797	38.0	35.0	39.0	33.0	39.0
13	36.06651152829542	38.0	35.0	39.0	32.0	39.0
14	37.10747449009265	39.0	35.0	41.0	32.0	41.0
15	37.303562252521566	39.0	35.0	41.0	32.0	41.0
16	37.42511551559332	39.0	35.0	41.0	33.0	41.0
17	37.34020736357596	39.0	35.0	41.0	33.0	41.0
18	37.24010890248383	39.0	35.0	41.0	32.0	41.0
19	37.20747236897114	39.0	35.0	41.0	32.0	41.0
20	37.0783265172348	38.0	35.0	41.0	32.0	41.0
21	36.92756130554345	38.0	35.0	41.0	32.0	41.0
22	36.849952890575445	38.0	35.0	41.0	32.0	41.0
23	36.79580202683424	38.0	35.0	41.0	32.0	41.0
24	36.662549752273534	38.0	35.0	41.0	31.0	41.0
25	36.545672877903456	38.0	35.0	40.0	31.0	41.0
26	36.51087998489214	38.0	35.0	40.0	31.0	41.0
27	36.47094850395931	38.0	35.0	40.0	31.0	41.0
28	36.48871939683515	38.0	35.0	40.0	31.0	41.0
29	36.44153846995979	38.0	35.0	40.0	31.0	41.0
30	36.38574407916299	38.0	35.0	40.0	31.0	41.0
31	36.27374236310727	38.0	35.0	40.0	31.0	41.0
32	36.13693515731537	38.0	35.0	41.0	30.0	41.0
33	36.04729348316206	38.0	35.0	41.0	30.0	41.0
34	35.93455382208991	38.0	35.0	41.0	30.0	41.0
35	35.7706181563896	38.0	35.0	41.0	30.0	41.0
36	35.606838837871734	38.0	35.0	40.0	29.0	41.0
37	35.54873926695409	38.0	35.0	40.0	29.0	41.0
38	35.42692496908978	38.0	34.0	40.0	28.0	41.0
39	35.36073639865042	37.0	34.0	40.0	28.0	41.0
40	35.15401531723425	37.0	34.0	40.0	27.0	41.0
41	35.10679402062688	37.0	34.0	40.0	27.0	41.0
42	35.07281023283756	37.0	34.0	40.0	27.0	41.0
43	34.992770396807096	37.0	34.0	40.0	27.0	41.0
44	34.84374553110488	37.0	34.0	40.0	26.0	41.0
45	34.72371502800907	36.0	34.0	40.0	26.0	41.0
46	34.647096697824345	36.0	34.0	40.0	25.0	41.0
47	34.55619535118588	36.0	34.0	40.0	25.0	41.0
48	34.44579918726834	36.0	34.0	40.0	24.0	41.0
49	34.36003232315492	36.0	34.0	40.0	24.0	41.0
50	34.22337941184722	35.0	34.0	40.0	24.0	41.0
51	33.84372534623888	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	13.0
12	17.0
13	28.0
14	50.0
15	116.0
16	268.0
17	593.0
18	1244.0
19	2453.0
20	4189.0
21	6637.0
22	10032.0
23	14862.0
24	21375.0
25	30455.0
26	39723.0
27	46837.0
28	51376.0
29	57639.0
30	67188.0
31	82174.0
32	103112.0
33	136444.0
34	247231.0
35	415762.0
36	192208.0
37	251491.0
38	387288.0
39	751948.0
40	225.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.13311542801153	24.011711327678377	29.39002019170833	13.46515305260176
2	30.560331880251056	25.751167814239018	30.210825793658667	13.477674511851253
3	28.27023909144839	25.91634159909298	31.6583885908295	14.155030718629124
4	24.991395773220635	28.079748694997097	30.873778901135896	16.055076630646376
5	23.319883598325273	32.73431037207892	29.496452595329014	14.449353434266786
6	22.74735184821528	40.37845597407031	26.623667200140133	10.25052497757427
7	87.55284842670945	3.8005365753193145	6.770414597147707	1.8762004008235424
8	88.70058043463833	2.9961867709072445	6.21300439072153	2.0902284037329
9	84.03359993321888	5.429181568685678	7.877742661432742	2.6594758366626956
10	47.56443932942454	27.799418539012557	14.163412569766082	10.472729561796823
11	40.807196212634906	21.729624062002433	22.93370263655404	14.52947708880862
12	37.0776829963373	21.876870948914497	25.140387453634677	15.90505860111352
13	22.402190639559187	35.32591032035093	25.94251350162266	16.32938553846722
14	16.979235588860963	37.829278456042495	28.64557496419752	16.54591099089902
15	16.32333007866624	24.975111033868835	43.129687421954706	15.571871465510224
16	19.648940705074473	22.91149928395043	40.39686183493432	17.04269817604077
17	20.148259551375958	21.776357158545622	27.72511086281065	30.35027242726777
18	23.24978395351049	24.68489371470642	32.73372877424493	19.331593557538156
19	26.89972090146296	26.061091036482605	26.368174692830813	20.67101336922362
20	29.9762365967358	25.921644402873508	25.33611223059191	18.76600676979879
21	24.406239928949272	26.94737771221308	29.674455744168114	18.971926614669542
22	24.176748265983164	24.15837661675645	27.330650684814344	24.334224432446046
23	20.295403803376143	29.523787693526682	26.27771912382628	23.903089379270895
24	20.712785778359226	25.760233898121847	35.248763078253646	18.278217245265278
25	19.73009070873512	26.07313353280999	32.857540689610815	21.33923506884408
26	19.80703268100864	32.02092931123079	26.991065973037127	21.180972034723446
27	19.459339811192816	31.932834345199524	29.208322186041514	19.399503657566143
28	16.91953628178347	28.64673815986551	35.88944440985268	18.54428114849835
29	17.546122418817493	25.695437057087588	35.24072334348963	21.517717180605285
30	19.787737317574997	27.29825226429721	33.21238379162102	19.701626626506766
31	26.559007205655043	26.85928274618181	26.29821189456521	20.283498153597932
32	27.093392980182568	26.55339649713888	27.622475950929566	18.73073457174899
33	24.789855017923475	26.852406207085778	27.434038252715894	20.923700522274853
34	19.8957092448739	27.754430235971345	29.488789188575225	22.861071330579524
35	20.231941216196336	26.600574344966887	30.857015198861987	22.310469239974793
36	27.729113624374012	24.929917461003864	27.866986522667602	19.473982391954518
37	20.602624306273526	29.422350188950873	29.736720924042636	20.23830458073296
38	21.255484980749113	30.638402836555272	25.070082538996136	23.036029643699482
39	20.528761381356436	29.27500066712693	28.026960138652925	22.169277812863715
40	24.051362615301773	26.1273247662832	26.541011884438564	23.280300733976468
41	19.202684108215514	25.672036297178703	28.666717756045024	26.458561838560758
42	22.92104433075537	26.41702891088621	26.14600432024556	24.51592243811286
43	22.823678011017513	26.43738483507596	27.06725528929018	23.67168186461634
44	20.6434730012022	28.526381619866285	28.64112745134934	22.189017927582174
45	19.082053875117943	32.41111303456539	25.184315195919783	23.322517894396885
46	22.843349702461392	29.0656938701641	27.149671123530695	20.941285303843816
47	21.670677410945398	27.691378188439064	28.0053725955206	22.632571805094933
48	22.97130122593981	25.566903935775176	29.643562635691907	21.818232202593105
49	22.063632276900783	24.04441765293115	30.627968287180696	23.263981782987376
50	20.140185604974643	30.149552751265656	28.348857433949306	21.36140420981039
51	19.392695541744697	30.80227657919207	26.183773967817796	23.621253911245432
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4077.0
1	5224.5
2	6372.0
3	15609.5
4	24847.0
5	18414.5
6	11982.0
7	11966.0
8	11950.0
9	12340.5
10	12731.0
11	12474.5
12	12218.0
13	12263.0
14	12308.0
15	11178.0
16	10048.0
17	10465.5
18	10883.0
19	11113.5
20	11344.0
21	12910.5
22	14477.0
23	14269.5
24	14062.0
25	17771.0
26	23169.5
27	24859.0
28	31876.0
29	38893.0
30	43396.5
31	47900.0
32	56202.0
33	64504.0
34	74802.0
35	85100.0
36	88887.0
37	92674.0
38	107579.5
39	122485.0
40	152313.5
41	182142.0
42	215747.5
43	249353.0
44	257321.0
45	265289.0
46	258869.0
47	252449.0
48	242780.5
49	233112.0
50	222442.0
51	211772.0
52	194363.5
53	176955.0
54	163843.0
55	150731.0
56	139840.5
57	128950.0
58	121548.0
59	114146.0
60	102729.5
61	91313.0
62	79963.5
63	68614.0
64	58845.5
65	49077.0
66	41366.0
67	33655.0
68	28646.5
69	23638.0
70	18431.0
71	13224.0
72	11905.5
73	10587.0
74	8299.5
75	5158.0
76	4304.0
77	2810.5
78	1317.0
79	998.5
80	680.0
81	442.5
82	205.0
83	172.0
84	139.0
85	95.5
86	52.0
87	39.0
88	26.0
89	21.5
90	17.0
91	17.0
92	17.0
93	12.5
94	8.0
95	5.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2922982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.404908080326358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.10005533549428	13.30438953088165
2	8.953585138551478	2.937654823746263
3	2.833610816493348	1.3945537493997568
4	1.2520470743956102	0.821588686708061
5	0.71052404956399	0.5828040860979252
6	0.4549332605793983	0.4477882993492914
7	0.30771799044519965	0.3533659743541372
8	0.22708235178295144	0.2980212086130923
9	0.18737648538764165	0.2766504617278989
>10	1.639086218391856	5.9944944020584074
>50	0.5761745757262026	6.952398573738868
>100	1.734937172287235	63.35445969747592
>500	0.02014197216957973	2.130532643385613
>1k	0.0025177465211974662	0.6658796510272874
>5k	0.0	0.0
>10k+	2.0981221009978886E-4	0.4854182114358438
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14103	0.4824867207529845	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4606	0.15757880137476044	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.842327458739055E-5	0.0	0.0	0.03879599669105044	0.0
2	6.842327458739055E-5	0.0	0.0	0.18207433367704626	0.0
3	6.842327458739055E-5	0.0	0.0	0.2726325375934576	0.0
4	6.842327458739055E-5	0.0	0.0	0.49576083602293824	0.0
5	6.842327458739055E-5	0.0	0.0	0.8356876641730945	0.0
6	6.842327458739055E-5	0.0	0.0	1.19727045873016	0.0
7	6.842327458739055E-5	0.0	0.0	1.3714419041923624	0.0
8	6.842327458739055E-5	0.0	0.0	1.7261823712906887	0.0
9	6.842327458739055E-5	0.0	0.0	1.841680858794204	0.0
10	6.842327458739055E-5	0.0	0.0	2.0565983642731975	0.0
11	6.842327458739055E-5	0.0	0.0	2.383627405163631	0.0
12	6.842327458739055E-5	0.0	0.0	2.6338513203297182	0.0
13	6.842327458739055E-5	0.0	0.0	2.7322782008236794	0.0
14	6.842327458739055E-5	0.0	0.0	2.7726821444675336	0.0
15	6.842327458739055E-5	0.0	0.0	2.8373763505899112	0.0
16	6.842327458739055E-5	0.0	0.0	2.9812020737726064	0.0
17	6.842327458739055E-5	0.0	0.0	3.139054568245716	0.0
18	6.842327458739055E-5	0.0	0.0	3.370701564361327	0.0
19	6.842327458739055E-5	0.0	0.0	3.484078930352633	0.0
20	6.842327458739055E-5	0.0	0.0	3.6069671315115865	0.0
21	6.842327458739055E-5	0.0	0.0	3.7659486100153883	0.0
22	6.842327458739055E-5	0.0	0.0	3.9216799829762894	0.0
23	6.842327458739055E-5	0.0	0.0	4.0886669846068155	0.0
24	6.842327458739055E-5	0.0	0.0	4.217644857204047	0.0
25	6.842327458739055E-5	0.0	0.0	4.324453588834964	0.0
26	6.842327458739055E-5	0.0	0.0	4.438686245758612	0.0
27	6.842327458739055E-5	0.0	0.0	4.548779294569724	0.0
28	6.842327458739055E-5	0.0	0.0	4.656614375319451	0.0
29	6.842327458739055E-5	0.0	0.0	4.774986640355637	0.0
30	6.842327458739055E-5	0.0	0.0	4.934036542134026	0.0
31	6.842327458739055E-5	0.0	0.0	5.074270043400883	0.0
32	6.842327458739055E-5	0.0	0.0	5.197329302746305	0.0
33	6.842327458739055E-5	0.0	0.0	5.323364974536278	0.0
34	6.842327458739055E-5	0.0	0.0	5.450050667434832	0.0
35	6.842327458739055E-5	0.0	0.0	5.610811151077906	0.0
36	6.842327458739055E-5	0.0	0.0	5.7432443990418	0.0
37	6.842327458739055E-5	0.0	0.0	5.8873096036855515	0.0
38	6.842327458739055E-5	0.0	0.0	6.028535242433925	0.0
39	6.842327458739055E-5	0.0	0.0	6.222959977173995	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACG	20	7.0350745E-4	45.000004	2
ACTACGT	20	7.0350745E-4	45.000004	45
CGAATTA	20	7.0350745E-4	45.000004	2
ACGACGT	20	7.0350745E-4	45.000004	27
ATATGCG	45	3.8562575E-10	45.0	1
ATCGTAC	25	3.8922895E-5	45.0	12
ATATCGG	50	2.1827873E-11	45.0	2
CGACTCG	25	3.8922895E-5	45.0	21
AGTCGAC	35	1.212793E-7	45.0	41
TAATCGC	35	1.212793E-7	45.0	24
CCTTACG	60	0.0	44.999996	18
ACACCGT	30	2.1666565E-6	44.999996	10
CGACATA	30	2.1666565E-6	44.999996	18
CGTTAGT	30	2.1666565E-6	44.999996	19
CGAATCG	30	2.1666565E-6	44.999996	45
CGTAAGA	30	2.1666565E-6	44.999996	28
TATCGCC	55	1.8189894E-12	44.999996	33
CGGTCTA	400	0.0	44.4375	31
TCACGAC	410	0.0	43.90244	25
CCCGTAG	75	0.0	42.0	37
>>END_MODULE