FastQCFastQC Report
Thu 26 May 2016
SRR1547501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547501_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2983693
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142400.47726089782025155No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33460.11214290478276417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG502.1827873E-1145.01
CGCGAAC453.8562575E-1045.028
TCGCTAA453.8562575E-1045.013
ACGGATA302.1666729E-644.99999636
CGTTAGG5450.042.110092
ATCGTAC556.184564E-1140.9090911
CGGTCTA5200.040.67307731
CGACGGT5400.040.41666428
CGTTTTT72600.040.1342961
CCGCTCG2300.040.10869219
ACGGTCT5350.039.9532730
CATACCG403.4607E-739.37500423
TAGGGAC26650.038.8367735
ACGGGAC8300.038.765065
GACCGAT14400.038.759
CACGAAT700.038.57142635
CCGCGTA356.251643E-638.57142645
GTCGATC1000.038.2528
ACGGGTA4650.038.2258075
TAAGGGA40500.037.9444434
Produced by FastQC (version 0.11.5)