FastQCFastQC Report
Thu 26 May 2016
SRR1547500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547500_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1927911
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86880.4506432091522897No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24770.12848103465357064No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACC253.8917926E-545.011
CTATCGA207.0344785E-445.038
CGACAAC207.0344785E-445.035
TCGATAG502.1827873E-1145.01
TCGAGTA253.8917926E-545.012
GTGCGTA453.8562575E-1045.018
TACGTCC453.8562575E-1045.044
TCTATCG207.0344785E-445.037
TAATCGC207.0344785E-445.027
CCCCGTA302.1662709E-644.99999638
TAATGCG600.044.9999961
ACGGTCC302.1662709E-644.99999630
GCCGATT1150.043.043489
CGACGGT2100.042.85714328
CGTTTTT47050.042.2263531
TATTGCG1100.040.9090921
TATCGGA556.184564E-1140.90909217
GGCGATA4700.040.691498
ACTTCGA501.0822987E-940.516
TAAGACG950.040.2631571
Produced by FastQC (version 0.11.5)