FastQCFastQC Report
Thu 26 May 2016
SRR1547499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547499_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences415943
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22530.5416607564017185No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9190.22094373507908538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCTCG253.8864975E-545.0000041
TATTGCG253.8864975E-545.0000041
AACGGAT253.8864975E-545.00000425
TCGTCCC950.045.00000438
GCGATAT502.1827873E-1145.0000049
CGATACA253.8864975E-545.00000410
TACAACG253.8864975E-545.0000041
TTTAGCG351.2094279E-745.01
TATCACG207.028091E-445.01
CGGGTCC207.028091E-445.06
AGCCGTT207.028091E-445.045
TACTTAC207.028091E-445.021
ATAGGCA207.028091E-445.018
CGAAAGT207.028091E-445.020
CGACGGT351.2094279E-745.028
ACGCATG453.8380676E-1045.01
CGTGCGG406.7975634E-945.02
TTTCGCA406.7975634E-945.013
ACCGAAC207.028091E-445.041
TACGGGT351.2094279E-745.04