FastQCFastQC Report
Thu 26 May 2016
SRR1547498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547498_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences6893782
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT823611.1947143092137233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT380100.042.058011
TACGGGA25800.039.1569754
TAGACGG9950.037.9899522
ATAGGGA84100.037.455414
GTAGGGA88950.037.3102874
TAGGGAC74050.037.282245
TAACGGG18500.037.0945933
AACGGGA21500.036.941864
CGGTCTA7250.036.93103831
ACGGGAT27400.036.7883225
TAGGGAT86500.036.702315
GACCGAT31050.036.4492769
GGGCGAT91450.036.191917
TATGGGA69650.036.1809044
AAGGGAT92050.036.078225
TAAGGGA87800.036.0307544
AGGGATT121450.035.866616
CTACGGG12950.035.7915043
ATAACGG5500.035.5909082
TACGGGC9250.035.513514
Produced by FastQC (version 0.11.5)