##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547498_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	6893782
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.514159861742073	31.0	31.0	33.0	30.0	34.0
2	31.93689690216488	31.0	31.0	34.0	30.0	34.0
3	31.887972233528707	33.0	31.0	34.0	30.0	34.0
4	35.723137459235005	37.0	35.0	37.0	35.0	37.0
5	35.71339317083134	37.0	35.0	37.0	33.0	37.0
6	35.793003463120826	37.0	35.0	37.0	35.0	37.0
7	36.06943329510565	37.0	35.0	37.0	35.0	37.0
8	36.07032409786094	37.0	35.0	37.0	35.0	37.0
9	37.91821107775093	39.0	38.0	39.0	35.0	39.0
10	37.42976960977298	39.0	37.0	39.0	35.0	39.0
11	37.09588176707648	39.0	37.0	39.0	33.0	39.0
12	36.69999065824826	39.0	35.0	39.0	33.0	39.0
13	36.534227656168994	39.0	35.0	39.0	32.0	39.0
14	37.64812348287196	40.0	36.0	41.0	33.0	41.0
15	37.7577634453773	40.0	36.0	41.0	33.0	41.0
16	37.8000740377343	40.0	36.0	41.0	33.0	41.0
17	37.70930296316303	40.0	36.0	41.0	33.0	41.0
18	37.54211751981713	39.0	36.0	41.0	32.0	41.0
19	37.461804565331484	39.0	36.0	41.0	32.0	41.0
20	37.29392385776052	39.0	35.0	41.0	32.0	41.0
21	37.207474358777226	39.0	35.0	41.0	32.0	41.0
22	37.13367031333454	39.0	35.0	41.0	32.0	41.0
23	37.014847147763014	39.0	35.0	41.0	32.0	41.0
24	36.870195779326934	39.0	35.0	41.0	31.0	41.0
25	36.706089777715626	38.0	35.0	41.0	31.0	41.0
26	36.67843442104784	38.0	35.0	41.0	31.0	41.0
27	36.62028158128586	38.0	35.0	41.0	31.0	41.0
28	36.55737489233051	38.0	35.0	41.0	31.0	41.0
29	36.45407223495028	38.0	35.0	41.0	31.0	41.0
30	36.359409972639114	38.0	35.0	40.0	30.0	41.0
31	36.23897985169824	38.0	35.0	41.0	30.0	41.0
32	36.06810441641468	38.0	35.0	41.0	30.0	41.0
33	35.883650077707706	38.0	35.0	41.0	29.0	41.0
34	35.638001317709204	38.0	35.0	41.0	27.0	41.0
35	35.379062030101906	38.0	34.0	41.0	26.0	41.0
36	35.242367397170376	38.0	34.0	40.0	25.0	41.0
37	35.18829852757166	38.0	34.0	40.0	25.0	41.0
38	35.01942460611606	38.0	34.0	40.0	24.0	41.0
39	34.88202208889112	38.0	34.0	40.0	24.0	41.0
40	34.70361813007722	38.0	34.0	40.0	23.0	41.0
41	34.58035139492372	37.0	34.0	40.0	23.0	41.0
42	34.53123524938851	37.0	33.0	40.0	23.0	41.0
43	34.40859574613761	37.0	33.0	40.0	23.0	41.0
44	34.24296300637299	37.0	33.0	40.0	23.0	41.0
45	34.13753742140381	37.0	33.0	40.0	23.0	41.0
46	34.06319129325529	36.0	33.0	40.0	23.0	41.0
47	33.97366525370254	36.0	33.0	40.0	22.0	41.0
48	33.81964529774803	36.0	33.0	40.0	22.0	41.0
49	33.70020360376931	36.0	33.0	40.0	21.0	41.0
50	33.59707327559821	35.0	33.0	40.0	21.0	41.0
51	33.22979084630178	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	14.0
10	20.0
11	35.0
12	36.0
13	65.0
14	125.0
15	337.0
16	781.0
17	1863.0
18	3870.0
19	7127.0
20	12252.0
21	19554.0
22	29086.0
23	41677.0
24	60382.0
25	88202.0
26	118654.0
27	138059.0
28	143928.0
29	151563.0
30	168035.0
31	197997.0
32	240671.0
33	310217.0
34	505795.0
35	652531.0
36	535804.0
37	699179.0
38	1035318.0
39	1730163.0
40	438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.310435694079096	23.26545864084475	27.57654651684663	16.84755914822952
2	33.18542709937738	24.946799884301534	28.783199120598823	13.084573895722261
3	28.606257058897423	24.9834125883296	32.996503225660454	13.413827127112521
4	25.485183604587437	27.12776528181483	32.66973049046227	14.717320623135457
5	22.629015538930588	32.21545154749599	31.328202719494175	13.827330194079243
6	22.35883002972824	40.39161667717372	27.087990307787514	10.16156298531053
7	86.7182919332233	3.335426040452106	8.449657967136181	1.4966240591884106
8	87.31929730298985	2.6130504271820607	8.49139122763093	1.5762610421971568
9	82.56396851539547	5.194376613591785	9.657877200062318	2.5837776709504303
10	43.86171480328215	31.240761602267085	14.928046752856414	9.969476841594354
11	32.228927459557035	22.35945378023268	27.13918136662865	18.272437393581637
12	29.79339932710376	19.73790293919941	31.245809629605347	19.222888104091485
13	25.75742894103701	22.0111253880671	32.236586535518526	19.99485913537736
14	21.110879340251838	25.728823452786877	33.21900808583735	19.941289121123933
15	20.72809961208521	24.17197120535578	36.03371850168746	19.066210680871546
16	25.943988365167336	22.84114873374296	32.50693160880341	18.707931292286297
17	26.093470898847688	22.457542173512305	29.451772626404487	21.99721430123552
18	25.64714114835659	23.67121559689587	31.32406855917405	19.35757469557349
19	27.160287342999823	24.131195329356224	28.55479038936827	20.153726938275682
20	27.715628373511088	25.356923093883733	28.149163985748316	18.778284546856863
21	27.90492939869581	23.24681865483997	30.36173757742847	18.48651436903575
22	26.735730836861393	20.582562662991084	31.20768831970608	21.474018180441448
23	24.77610983347022	22.998290343384806	30.83873264341692	21.38686717972805
24	23.981973900538193	23.631498646171288	31.77385069617809	20.612676757112425
25	24.66643128546856	23.804466111635094	29.92998618175045	21.599116421145897
26	23.508474738539746	25.612965423043548	28.449333036640844	22.42922680177586
27	22.28685212268099	24.992406200254084	31.303673368261425	21.4170683088035
28	21.47648417080784	25.23819291065485	31.89268242018677	21.39264049835054
29	22.748848164911507	24.488053147024377	31.72558981412525	21.03750887393886
30	23.901437556337	23.347532602568517	31.469300885928796	21.281728955165686
31	26.1854233278627	22.964564298667987	29.801537095312845	21.048475278156463
32	27.19035791964411	23.868552849509893	28.58507855339783	20.356010677448168
33	26.41445580959769	23.65132810988221	28.446243295770014	21.487972784750085
34	24.007605694522976	24.84057372281282	29.496203970476582	21.655616612187618
35	23.734083845413156	25.340517005034393	29.542347001979465	21.383052147572986
36	26.235526449777495	25.601955501348893	27.700440774019253	20.462077274854355
37	24.774383640213745	26.608311664047395	26.915516040396987	21.701788655341872
38	24.50144202413131	27.13877520350948	25.939941239801318	22.41984153255789
39	24.149748280406893	25.878973254448717	26.828756696977074	23.142521768167313
40	25.313724744994836	24.704218961377077	27.404391377621167	22.577664916006917
41	22.974645847518822	24.81045962869148	28.52438617873324	23.690508345056458
42	23.481203786252596	25.321891524855296	27.981012454411818	23.21589223448029
43	23.307365971247712	24.536183476646055	28.448027512329226	23.708423039777003
44	22.862689884884666	24.889719460232424	29.172129318855745	23.07546133602716
45	23.176465400269404	25.769323718098423	27.956628161435916	23.097582720196257
46	24.287611647713838	25.571623819842287	27.99736051995842	22.143404012485455
47	23.621939307045103	25.57936993075789	28.839438206778222	21.959252555418782
48	23.466552902311097	25.496091985502296	29.280023650298197	21.75733146188841
49	23.953165330728474	24.524245182107588	29.389063941969734	22.133525545194203
50	23.023153328608302	25.30915540990417	29.053732769617607	22.61395849186992
51	22.27674156217879	26.500417332604947	28.646829853337398	22.576011251878867
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6858.0
1	10172.0
2	13486.0
3	73487.0
4	133488.0
5	92215.5
6	50943.0
7	46836.5
8	42730.0
9	43010.0
10	43290.0
11	43048.0
12	42806.0
13	41147.5
14	39489.0
15	37793.0
16	36097.0
17	34472.0
18	32847.0
19	32089.5
20	31332.0
21	32379.5
22	33427.0
23	36158.5
24	38890.0
25	43041.0
26	57259.0
27	67326.0
28	72885.0
29	78444.0
30	92234.5
31	106025.0
32	115669.0
33	125313.0
34	144023.0
35	162733.0
36	180138.0
37	197543.0
38	213978.0
39	230413.0
40	257205.5
41	283998.0
42	312730.5
43	341463.0
44	357607.0
45	373751.0
46	389905.0
47	406059.0
48	417473.5
49	428888.0
50	431597.5
51	434307.0
52	426791.0
53	419275.0
54	416294.5
55	413314.0
56	414073.5
57	414833.0
58	405701.5
59	396570.0
60	380741.5
61	364913.0
62	333747.0
63	302581.0
64	269664.0
65	236747.0
66	205222.0
67	173697.0
68	148677.0
69	123657.0
70	105828.5
71	88000.0
72	72275.5
73	56551.0
74	45290.0
75	26985.0
76	19941.0
77	15085.0
78	10229.0
79	7483.5
80	4738.0
81	3744.5
82	2751.0
83	2152.5
84	1554.0
85	1091.5
86	629.0
87	454.0
88	279.0
89	249.5
90	220.0
91	154.5
92	89.0
93	58.5
94	28.0
95	20.0
96	12.0
97	8.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6893782.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.17043690060394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.09187193824049	15.098156160068616
2	6.497172686238185	2.3611293265523665
3	1.9888813557893827	1.0841652953447576
4	1.0096932392392812	0.7338626917025501
5	0.6695689206505883	0.6083179911643499
6	0.4727978092241101	0.5154565655550282
7	0.34467504904422364	0.43840273609094427
8	0.27948977523848273	0.40627610602678615
9	0.22864567002261493	0.37391325477680104
>10	2.5433875677519717	11.105679767913216
>50	1.2454113636321524	16.71476675186676
>100	1.619779489929422	47.591124993114704
>500	0.005723220430250147	0.6801169379084342
>1k	0.0026600883547192307	0.8961010053741777
>5k	1.6121747604358973E-4	0.18619215397706027
>10k+	8.060873802179487E-5	1.2063382625632992
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82361	1.1947143092137233	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.8023302738612855E-5	0.0	0.0	0.008413378897098863	0.0
2	5.8023302738612855E-5	0.0	0.0	0.027546562975156452	0.0
3	5.8023302738612855E-5	0.0	0.0	0.05063983746512437	0.0
4	5.8023302738612855E-5	0.0	0.0	0.08049282672414068	0.0
5	5.8023302738612855E-5	0.0	0.0	0.1527028269823444	0.0
6	7.252912842326606E-5	0.0	0.0	0.3084518773584659	0.0
7	7.252912842326606E-5	0.0	0.0	0.4092673658668058	0.0
8	7.252912842326606E-5	0.0	0.0	0.5901405063287467	0.0
9	7.252912842326606E-5	0.0	0.0	0.7205333734080944	0.0
10	7.252912842326606E-5	0.0	0.0	0.8695227090151676	0.0
11	7.252912842326606E-5	0.0	0.0	0.9910089991241382	0.0
12	7.252912842326606E-5	0.0	0.0	1.0720530472242957	0.0
13	7.252912842326606E-5	0.0	0.0	1.1172096825806213	0.0
14	7.252912842326606E-5	0.0	0.0	1.150892209820386	0.0
15	7.252912842326606E-5	0.0	0.0	1.1741160367415158	0.0
16	7.252912842326606E-5	0.0	0.0	1.2067396387063007	0.0
17	7.252912842326606E-5	0.0	0.0	1.2429896970922492	0.0
18	8.703495410791928E-5	0.0	0.0	1.288044791668782	0.0
19	8.703495410791928E-5	0.0	0.0	1.3189422003770934	0.0
20	8.703495410791928E-5	0.0	0.0	1.3556274335335814	1.4505825684653214E-5
21	8.703495410791928E-5	0.0	0.0	1.3968384843036812	1.4505825684653214E-5
22	8.703495410791928E-5	0.0	0.0	1.4466950071818343	1.4505825684653214E-5
23	8.703495410791928E-5	0.0	0.0	1.4992206019859635	1.4505825684653214E-5
24	8.703495410791928E-5	0.0	0.0	1.5431877596361474	1.4505825684653214E-5
25	8.703495410791928E-5	0.0	0.0	1.58219392490218	1.4505825684653214E-5
26	8.703495410791928E-5	0.0	0.0	1.6230278242044787	1.4505825684653214E-5
27	8.703495410791928E-5	0.0	0.0	1.6708970489638344	1.4505825684653214E-5
28	1.015407797925725E-4	0.0	0.0	1.7157200503294128	1.4505825684653214E-5
29	1.015407797925725E-4	0.0	0.0	1.7687388432068203	1.4505825684653214E-5
30	1.015407797925725E-4	0.0	0.0	1.8370757880072215	2.9011651369306427E-5
31	1.015407797925725E-4	0.0	0.0	1.891472634324671	2.9011651369306427E-5
32	1.015407797925725E-4	0.0	0.0	1.952135997337891	2.9011651369306427E-5
33	1.015407797925725E-4	0.0	0.0	2.008462118471399	2.9011651369306427E-5
34	1.015407797925725E-4	0.0	0.0	2.0726649029516744	2.9011651369306427E-5
35	1.015407797925725E-4	1.4505825684653214E-5	0.0	2.143424320641413	2.9011651369306427E-5
36	1.015407797925725E-4	1.4505825684653214E-5	0.0	2.2091937342956305	2.9011651369306427E-5
37	1.015407797925725E-4	1.4505825684653214E-5	0.0	2.2822595782692288	2.9011651369306427E-5
38	1.015407797925725E-4	1.4505825684653214E-5	0.0	2.3736752917339135	2.9011651369306427E-5
39	1.015407797925725E-4	1.4505825684653214E-5	0.0	2.5387660938509513	2.9011651369306427E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	38010	0.0	42.05801	1
TACGGGA	2580	0.0	39.156975	4
TAGACGG	995	0.0	37.989952	2
ATAGGGA	8410	0.0	37.45541	4
GTAGGGA	8895	0.0	37.310287	4
TAGGGAC	7405	0.0	37.28224	5
TAACGGG	1850	0.0	37.094593	3
AACGGGA	2150	0.0	36.94186	4
CGGTCTA	725	0.0	36.931038	31
ACGGGAT	2740	0.0	36.788322	5
TAGGGAT	8650	0.0	36.70231	5
GACCGAT	3105	0.0	36.449276	9
GGGCGAT	9145	0.0	36.19191	7
TATGGGA	6965	0.0	36.180904	4
AAGGGAT	9205	0.0	36.07822	5
TAAGGGA	8780	0.0	36.030754	4
AGGGATT	12145	0.0	35.86661	6
CTACGGG	1295	0.0	35.791504	3
ATAACGG	550	0.0	35.590908	2
TACGGGC	925	0.0	35.51351	4
>>END_MODULE