FastQCFastQC Report
Thu 26 May 2016
SRR1547497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547497_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2730179
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98780.36180777890387406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA207.0349936E-445.038
CCGCGTA207.0349936E-445.038
ACGTACT406.8212103E-945.011
TCGTTAG1500.042.01
TCGATAG1400.040.178571
CTTAACG900.040.0000041
CGTAAGG4300.039.767442
TACGCGG2000.039.3752
ATCGACC403.4605546E-739.37522
CGGTCTA3700.038.91892231
ACCGTTC5100.038.8235345
GACCGAT11800.038.7076269
TATCGGC356.251448E-638.57142622
TATCGCC356.251448E-638.57142641
TAGGGTA13850.038.339355
GGTACCT19850.038.3123448
AGGGTAC16100.038.2919246
TATGGGA24950.038.2364734
GTAGGGC10550.038.1753584
CTACGGG7100.038.028173
Produced by FastQC (version 0.11.5)