##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547497_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2730179
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.501894198145983	31.0	31.0	33.0	30.0	34.0
2	31.934052675667054	31.0	31.0	34.0	30.0	34.0
3	32.03451824953602	33.0	31.0	34.0	30.0	34.0
4	35.76691821305489	37.0	35.0	37.0	35.0	37.0
5	35.7052797637078	37.0	35.0	37.0	33.0	37.0
6	35.75747853895294	37.0	35.0	37.0	33.0	37.0
7	36.062324484951354	37.0	35.0	37.0	35.0	37.0
8	36.06331050088657	37.0	35.0	37.0	35.0	37.0
9	37.937104123942056	39.0	38.0	39.0	35.0	39.0
10	37.35006569166344	39.0	37.0	39.0	34.0	39.0
11	37.03568007811942	39.0	37.0	39.0	33.0	39.0
12	36.487349364272454	39.0	35.0	39.0	33.0	39.0
13	36.249492798823816	39.0	35.0	39.0	32.0	39.0
14	37.30351489774114	40.0	35.0	41.0	32.0	41.0
15	37.464738758887236	40.0	35.0	41.0	32.0	41.0
16	37.560515995471356	40.0	35.0	41.0	33.0	41.0
17	37.47934512718763	39.0	35.0	41.0	33.0	41.0
18	37.40026459803551	39.0	35.0	41.0	32.0	41.0
19	37.36261761591456	39.0	35.0	41.0	32.0	41.0
20	37.21934605752956	39.0	35.0	41.0	32.0	41.0
21	37.092820287607516	39.0	35.0	41.0	32.0	41.0
22	37.017527788471014	39.0	35.0	41.0	32.0	41.0
23	36.9412130120406	39.0	35.0	41.0	32.0	41.0
24	36.80860778725498	38.0	35.0	41.0	31.0	41.0
25	36.67171676289357	38.0	35.0	41.0	31.0	41.0
26	36.62193467900823	38.0	35.0	41.0	31.0	41.0
27	36.55999295284302	38.0	35.0	41.0	31.0	41.0
28	36.53650804580945	38.0	35.0	41.0	31.0	41.0
29	36.49095022707302	38.0	35.0	41.0	31.0	41.0
30	36.45239121683963	38.0	35.0	40.0	30.0	41.0
31	36.38232218473587	38.0	35.0	41.0	30.0	41.0
32	36.25763695347448	38.0	35.0	41.0	30.0	41.0
33	36.16964492071765	38.0	35.0	41.0	30.0	41.0
34	36.07029502461194	38.0	35.0	41.0	30.0	41.0
35	35.913568670772136	38.0	35.0	41.0	29.0	41.0
36	35.768778530638464	38.0	35.0	41.0	29.0	41.0
37	35.715979062178704	38.0	35.0	41.0	28.0	41.0
38	35.54422878499908	38.0	34.0	40.0	27.0	41.0
39	35.458995179436954	38.0	34.0	40.0	27.0	41.0
40	35.273504044972874	38.0	34.0	40.0	26.0	41.0
41	35.19160099026474	38.0	34.0	40.0	26.0	41.0
42	35.160885055522	38.0	34.0	40.0	26.0	41.0
43	35.081003113715255	37.0	34.0	40.0	26.0	41.0
44	34.93673858014438	37.0	34.0	40.0	26.0	41.0
45	34.81767642341399	37.0	34.0	40.0	25.0	41.0
46	34.77483857285548	37.0	34.0	40.0	25.0	41.0
47	34.67448324816798	37.0	34.0	40.0	24.0	41.0
48	34.535374054228676	36.0	34.0	40.0	24.0	41.0
49	34.42729066482454	36.0	33.0	40.0	24.0	41.0
50	34.31519874704186	36.0	33.0	40.0	24.0	41.0
51	33.94201772118238	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	7.0
11	18.0
12	15.0
13	36.0
14	54.0
15	135.0
16	326.0
17	716.0
18	1411.0
19	2671.0
20	4553.0
21	6814.0
22	10209.0
23	14509.0
24	20108.0
25	27752.0
26	36294.0
27	43619.0
28	48488.0
29	55182.0
30	64460.0
31	77511.0
32	95437.0
33	124603.0
34	209859.0
35	314378.0
36	191600.0
37	256041.0
38	395452.0
39	727695.0
40	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.2351102253735	23.880082587991485	30.03030204246681	12.854505144168202
2	29.767718526880472	25.689634269401385	31.102869079280147	13.439778124437996
3	29.731860072178417	25.979798394171226	30.509977550922486	13.778363982727873
4	25.493786304854005	27.914067172884998	31.120486971733357	15.471659550527638
5	23.616363615718967	32.597093450649204	29.176072338114096	14.61047059551773
6	22.855021593822237	41.66741448088202	25.604768039018687	9.872795886277054
7	90.0673545580711	3.556396851635003	4.9436319010585015	1.4326166892353944
8	90.97202051587094	2.640705975688774	4.855469183522399	1.5318043249178899
9	86.81643218265177	4.629147026623529	6.301747980626911	2.2526728100977995
10	44.65296964045214	28.431176124349356	14.873933174344977	12.04192106085352
11	35.691029782296326	24.10581870272975	22.55910693035145	17.644044584622474
12	33.17255022472885	21.01202888162278	27.48068899511717	18.334731898531196
13	24.80313561858032	28.05892946945969	27.487538362869245	19.650396549090736
14	19.533591020955036	31.64693597013236	30.12538738302507	18.69408562588753
15	18.698261176281846	26.01679230555945	37.24473743296685	18.04020908519185
16	23.447290452384255	22.125399103868283	35.26853001213473	19.158780431612726
17	23.024937192762817	23.279206235195566	26.747733390374773	26.948123181666844
18	24.31488924352579	25.141831359775313	30.444084435489394	20.099194961209506
19	28.310927598520095	25.81112813482193	25.221569721252706	20.656374545405264
20	29.060915053555096	26.470755214218556	25.31801028430737	19.15031944791898
21	26.98782753804787	25.536274361497906	28.487875703387942	18.988022397066274
22	25.626085322610713	22.093496433750314	27.94318614274009	24.337232100898877
23	23.001165857623253	26.571371327667524	27.19711784465414	23.23034497005508
24	22.766822248651096	25.739667618863088	31.43292069860621	20.06058943387961
25	23.35769925708168	25.323357919022893	29.749258198821394	21.569684625074036
26	22.413621963981118	28.671013878577188	25.947529447702877	22.967834709738813
27	21.414236941973403	29.12131402373251	28.69310034250502	20.771348691789076
28	19.769912522219236	27.101959248825807	32.177890167641024	20.95023806131393
29	19.9518053578172	25.82079783047192	32.46175433918436	21.76564247252653
30	21.755313479445853	26.480534792773664	31.472332033906937	20.291819693873553
31	27.51010098605256	25.374673235710922	26.194143314412717	20.9210824638238
32	27.808799349786224	25.801055535186524	26.57554687806184	19.814598236965413
33	25.28383670081705	24.95283276298001	27.185763277792407	22.57756725841053
34	21.641181768667916	26.2864449547081	28.7986611866841	23.27371208993989
35	22.8658267461584	26.311534884709026	28.69060966332244	22.132028705810132
36	27.487281969423982	25.00766433263167	26.76601058025866	20.73904311768569
37	23.106433680722034	27.92520197393651	27.909781739585572	21.058582605755884
38	23.65570169574962	29.26672573483277	24.67695341587493	22.40061915354268
39	21.746303081226543	28.298327692067076	26.238169731728213	23.717199494978168
40	24.670323813933077	25.33929094026436	26.732679432374212	23.257705813428352
41	21.764030856584863	24.390561937513986	27.92670370697306	25.918703498928092
42	23.082589090312393	26.505002052978945	25.873981156546876	24.538427700161787
43	22.61822393330254	25.310758012569874	27.31513208474609	24.755885969381495
44	22.03485559005472	26.847140791867492	28.54450202715646	22.573501590921328
45	21.34413164851096	29.304085922571378	25.53535867062196	23.816423758295702
46	23.66471209396893	27.06020374488266	27.070166461612956	22.20491769953545
47	23.65925457634829	26.65561488825458	26.805202149749157	22.879928385647975
48	23.329862254452912	26.310985470183457	27.841104923889603	22.518047351474024
49	22.670088664516136	25.300648785299423	29.16563346212831	22.863629088056133
50	22.065366410041246	27.93249087330904	27.975857993193852	22.02628472345586
51	22.04672294380698	28.87470015702267	26.355085142769024	22.72349175640132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2160.0
1	3722.0
2	5284.0
3	12081.5
4	18879.0
5	14129.5
6	9380.0
7	9266.5
8	9153.0
9	9165.0
10	9177.0
11	9016.0
12	8855.0
13	8605.5
14	8356.0
15	8355.5
16	8355.0
17	8260.0
18	8165.0
19	7890.5
20	7616.0
21	8386.5
22	9157.0
23	10547.0
24	11937.0
25	12666.0
26	18307.5
27	23220.0
28	28057.0
29	32894.0
30	38957.5
31	45021.0
32	49050.0
33	53079.0
34	63219.5
35	73360.0
36	76603.5
37	79847.0
38	92460.0
39	105073.0
40	126428.0
41	147783.0
42	168442.5
43	189102.0
44	195359.5
45	201617.0
46	202887.0
47	204157.0
48	201514.5
49	198872.0
50	201281.0
51	203690.0
52	192855.0
53	182020.0
54	175932.0
55	169844.0
56	165879.5
57	161915.0
58	147706.5
59	133498.0
60	123881.5
61	114265.0
62	101213.5
63	88162.0
64	78737.0
65	69312.0
66	55140.0
67	40968.0
68	36376.5
69	31785.0
70	26228.0
71	20671.0
72	16854.5
73	13038.0
74	10531.0
75	6154.0
76	4284.0
77	3205.0
78	2126.0
79	1777.5
80	1429.0
81	1050.0
82	671.0
83	497.5
84	324.0
85	219.0
86	114.0
87	82.0
88	50.0
89	40.5
90	31.0
91	29.0
92	27.0
93	25.5
94	24.0
95	16.5
96	9.0
97	5.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2730179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.145930006321645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.96642418942362	14.225365020266487
2	7.113904924057933	2.4394903179904843
3	2.0010847158831715	1.029313754257587
4	0.9393456726311037	0.6442382061869613
5	0.5667680374740413	0.48588825501750976
6	0.39183509429870755	0.40310262605196484
7	0.2993194205715508	0.3592476884256793
8	0.23067658333119828	0.31641316415153187
9	0.18615503417451454	0.28726210676525765
>10	2.2645347118227797	9.46221568574093
>50	1.2305290902732133	15.598509067436522
>100	1.801479871779293	53.159684559874464
>500	0.005366658296549	0.5989896158192809
>1k	0.00236132965048156	0.6267049396924478
>5k	2.1466633186196003E-4	0.36357499232289886
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9878	0.36180777890387406	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.098829051135475E-4	0.0	0.0	0.016995222657562013	0.0
2	1.098829051135475E-4	0.0	0.0	0.05541761181226579	0.0
3	1.098829051135475E-4	0.0	0.0	0.08116683924387376	0.0
4	1.098829051135475E-4	0.0	0.0	0.16519063402069975	0.0
5	1.098829051135475E-4	0.0	0.0	0.29488908968972366	0.0
6	1.098829051135475E-4	0.0	0.0	0.4413996298411203	0.0
7	1.098829051135475E-4	0.0	0.0	0.5159002395081055	0.0
8	1.098829051135475E-4	0.0	0.0	0.6979395856462158	0.0
9	1.098829051135475E-4	0.0	0.0	0.7503171037503402	0.0
10	1.098829051135475E-4	0.0	0.0	0.8484059103817002	0.0
11	1.098829051135475E-4	0.0	0.0	0.9806683005033736	0.0
12	1.098829051135475E-4	0.0	0.0	1.091576779397981	0.0
13	1.098829051135475E-4	0.0	0.0	1.1334055386112045	0.0
14	1.098829051135475E-4	0.0	0.0	1.1487891453271013	0.0
15	1.098829051135475E-4	0.0	0.0	1.173146522627271	0.0
16	1.098829051135475E-4	0.0	0.0	1.232629801928738	0.0
17	1.098829051135475E-4	0.0	0.0	1.3131007161068926	0.0
18	1.098829051135475E-4	0.0	0.0	1.4156580942128703	0.0
19	1.098829051135475E-4	0.0	0.0	1.4734931299376341	3.662763503784917E-5
20	1.098829051135475E-4	0.0	0.0	1.5401920533415574	3.662763503784917E-5
21	1.098829051135475E-4	0.0	0.0	1.6279152392572063	3.662763503784917E-5
22	1.098829051135475E-4	0.0	0.0	1.7252714931878093	3.662763503784917E-5
23	1.098829051135475E-4	0.0	0.0	1.8213457798920876	3.662763503784917E-5
24	1.098829051135475E-4	0.0	0.0	1.8956998790189215	3.662763503784917E-5
25	1.098829051135475E-4	0.0	0.0	1.9585162731088328	3.662763503784917E-5
26	1.098829051135475E-4	0.0	0.0	2.02038034868776	3.662763503784917E-5
27	1.098829051135475E-4	0.0	0.0	2.089057164383727	3.662763503784917E-5
28	1.4651054015139668E-4	0.0	0.0	2.1661217085033617	3.662763503784917E-5
29	1.4651054015139668E-4	0.0	0.0	2.256848360492114	3.662763503784917E-5
30	1.4651054015139668E-4	0.0	0.0	2.3614935137952493	3.662763503784917E-5
31	1.4651054015139668E-4	0.0	0.0	2.4510114538277525	3.662763503784917E-5
32	1.4651054015139668E-4	0.0	0.0	2.5411154360208617	3.662763503784917E-5
33	1.4651054015139668E-4	0.0	0.0	2.6274467718050722	3.662763503784917E-5
34	1.4651054015139668E-4	0.0	0.0	2.7230082716188204	3.662763503784917E-5
35	1.4651054015139668E-4	0.0	0.0	2.8371399823967587	3.662763503784917E-5
36	1.4651054015139668E-4	0.0	0.0	2.9320788124148636	3.662763503784917E-5
37	1.4651054015139668E-4	0.0	0.0	3.0331344574842896	3.662763503784917E-5
38	1.4651054015139668E-4	0.0	0.0	3.165543358146114	3.662763503784917E-5
39	1.4651054015139668E-4	0.0	0.0	3.4258193327250703	3.662763503784917E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	20	7.0349936E-4	45.0	38
CCGCGTA	20	7.0349936E-4	45.0	38
ACGTACT	40	6.8212103E-9	45.0	11
TCGTTAG	150	0.0	42.0	1
TCGATAG	140	0.0	40.17857	1
CTTAACG	90	0.0	40.000004	1
CGTAAGG	430	0.0	39.76744	2
TACGCGG	200	0.0	39.375	2
ATCGACC	40	3.4605546E-7	39.375	22
CGGTCTA	370	0.0	38.918922	31
ACCGTTC	510	0.0	38.82353	45
GACCGAT	1180	0.0	38.707626	9
TATCGGC	35	6.251448E-6	38.571426	22
TATCGCC	35	6.251448E-6	38.571426	41
TAGGGTA	1385	0.0	38.33935	5
GGTACCT	1985	0.0	38.312344	8
AGGGTAC	1610	0.0	38.291924	6
TATGGGA	2495	0.0	38.236473	4
GTAGGGC	1055	0.0	38.175358	4
CTACGGG	710	0.0	38.02817	3
>>END_MODULE