FastQCFastQC Report
Thu 26 May 2016
SRR1547496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547496_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3398968
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140930.4146258511406992No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37050.10900367405636063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG253.8924234E-545.020
TACGTTG1100.040.9090921
CGGGATA8200.040.6097566
CGGTCTA4850.039.43298731
CGTTTTT79600.039.0640681
TAGGGAT49700.038.7977835
TAGGGTA17200.038.3284875
ATAGGGA46950.038.2428134
CGTTAGG3950.038.1645552
ACGGGTA3600.038.1255
CTATGCG1600.037.968751
GGGCGAT50250.037.9253737
ACGGGAT14250.037.7368435
GGCGATT15400.037.5487028
ATAGGGC16300.037.5460134
TACGGGA11350.037.4669654
CGTAAGG4450.037.4157332
ATAACGC3100.037.01613211
CGAGGGA23350.036.905784
CGCATCG2450.036.73469521
Produced by FastQC (version 0.11.5)