##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547496_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3398968
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52789758538474	31.0	31.0	33.0	30.0	34.0
2	31.968823183978195	31.0	31.0	34.0	30.0	34.0
3	32.05700200766821	33.0	31.0	34.0	30.0	34.0
4	35.793869492151735	37.0	35.0	37.0	35.0	37.0
5	35.73321961254122	37.0	35.0	37.0	33.0	37.0
6	35.789108046913064	37.0	35.0	37.0	35.0	37.0
7	36.088062317738796	37.0	35.0	37.0	35.0	37.0
8	36.09218533390135	37.0	35.0	37.0	35.0	37.0
9	37.95407664914762	39.0	38.0	39.0	35.0	39.0
10	37.3803454460295	39.0	37.0	39.0	35.0	39.0
11	37.05244356522333	39.0	37.0	39.0	33.0	39.0
12	36.61129995928176	39.0	35.0	39.0	33.0	39.0
13	36.43842719319511	39.0	35.0	39.0	32.0	39.0
14	37.58100046837746	40.0	36.0	41.0	33.0	41.0
15	37.69992980222232	40.0	36.0	41.0	33.0	41.0
16	37.7433668101612	40.0	35.0	41.0	33.0	41.0
17	37.65012291966267	40.0	35.0	41.0	33.0	41.0
18	37.5572756201294	39.0	36.0	41.0	33.0	41.0
19	37.52819473440174	39.0	36.0	41.0	32.0	41.0
20	37.398132609662696	39.0	35.0	41.0	32.0	41.0
21	37.28868997884064	39.0	35.0	41.0	32.0	41.0
22	37.21332004302482	39.0	35.0	41.0	32.0	41.0
23	37.12189582249671	39.0	35.0	41.0	32.0	41.0
24	37.01366856057486	39.0	35.0	41.0	32.0	41.0
25	36.87085050521217	39.0	35.0	41.0	31.0	41.0
26	36.85484447043926	39.0	35.0	41.0	31.0	41.0
27	36.80673869245018	39.0	35.0	41.0	31.0	41.0
28	36.777220320991546	39.0	35.0	41.0	31.0	41.0
29	36.72071493465075	39.0	35.0	41.0	31.0	41.0
30	36.658687283904996	39.0	35.0	41.0	31.0	41.0
31	36.61146236151679	39.0	35.0	41.0	31.0	41.0
32	36.511552330001344	39.0	35.0	41.0	30.0	41.0
33	36.43164248677834	39.0	35.0	41.0	30.0	41.0
34	36.302569780003815	39.0	35.0	41.0	30.0	41.0
35	36.131503150368	38.0	35.0	41.0	30.0	41.0
36	36.027229147199975	38.0	35.0	41.0	29.0	41.0
37	35.983880107138404	38.0	35.0	41.0	29.0	41.0
38	35.842686956746874	38.0	35.0	41.0	29.0	41.0
39	35.75572173671538	38.0	35.0	41.0	28.0	41.0
40	35.594696684405385	38.0	35.0	40.0	27.0	41.0
41	35.52807205010462	38.0	35.0	40.0	27.0	41.0
42	35.49355216053814	38.0	34.0	40.0	27.0	41.0
43	35.38225514332586	38.0	34.0	40.0	27.0	41.0
44	35.23159970908817	38.0	34.0	40.0	26.0	41.0
45	35.11614996081163	38.0	34.0	40.0	26.0	41.0
46	35.04648293246656	37.0	34.0	40.0	26.0	41.0
47	34.95884750900861	37.0	34.0	40.0	26.0	41.0
48	34.81998330081366	37.0	34.0	40.0	25.0	41.0
49	34.69943553455049	37.0	34.0	40.0	24.0	41.0
50	34.61333322349607	37.0	34.0	40.0	24.0	41.0
51	34.224786170390544	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	9.0
11	9.0
12	16.0
13	34.0
14	61.0
15	128.0
16	299.0
17	684.0
18	1434.0
19	2698.0
20	4623.0
21	7324.0
22	10947.0
23	15888.0
24	22472.0
25	31900.0
26	41902.0
27	50101.0
28	56686.0
29	65210.0
30	76082.0
31	92100.0
32	115774.0
33	148850.0
34	249142.0
35	357382.0
36	243578.0
37	325207.0
38	512160.0
39	965968.0
40	295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.56420772422688	23.992811935858178	29.964948184272405	13.478032155642536
2	30.49140209616566	26.108101047141368	29.781363049019582	13.61913380767339
3	29.514223140670932	25.46372899068188	31.05245474508733	13.969593123559857
4	25.7039489633324	27.5357402599848	31.049689199780637	15.710621576902165
5	23.11045587954932	32.39018431476848	29.955533561951746	14.54382624373045
6	22.117683955836007	41.78653638398478	25.859702121349777	10.236077538829432
7	90.46525298267002	3.1147689533999734	4.954856885972448	1.4651211779575448
8	91.44022538605836	2.398669243134975	4.767888370823144	1.3932169999835244
9	86.91208625676971	4.812107675035481	5.995496280047355	2.2803097881474614
10	42.613081382348994	32.83199488786008	12.897473586100253	11.657450143690673
11	33.04611870426553	23.633585252935596	25.247133835917257	18.07316220688162
12	31.415417856243426	21.274781051189656	28.236688312452486	19.073112780114435
13	23.79119191472235	26.91616984920129	28.993300319391064	20.2993379166853
14	19.605274306789592	30.3021387668257	29.610605336678663	20.481981589706052
15	19.11459595971483	26.536554624815533	35.340638687978235	19.008210727491402
16	22.494092324493785	24.25495032609898	33.75156812303028	19.499389226376948
17	22.644020184950257	23.03349134207795	28.22989213196476	26.092596341007035
18	24.22741255581106	25.18502674929567	30.291253109767435	20.296307585125838
19	26.847972678766023	26.525433602199257	26.280153270051382	20.346440448983337
20	27.98349381341631	25.353901537172458	27.511674131677616	19.15093051773362
21	26.759857697983623	25.286204518547983	29.116425926928407	18.837511856539983
22	26.11527969666087	22.45831675967529	28.785001800546517	22.641401743117324
23	23.326933351534937	26.500161225407243	28.11382749116791	22.059077931889913
24	22.141308773721907	26.122958497991156	32.223074768576815	19.512657959710122
25	21.94068905620765	26.533288927698056	30.07203951316988	21.45398250292442
26	21.502614911349564	28.881030948217223	26.817610521781905	22.798743618651308
27	20.49272014329055	28.178994330043704	30.176747765792438	21.1515377608733
28	19.156991180852543	26.779157673740972	33.042411696726774	21.021439448679715
29	20.1898046701234	25.568819712336214	32.71893115792794	21.522444459612448
30	21.65166015096347	26.080886904495713	31.219240663636725	21.048212280904085
31	25.276701634143066	26.107571474635833	27.61188101800311	21.003845873217987
32	25.701183418025707	25.774440947958322	27.928712479787983	20.59566315422799
33	24.906471611383218	25.544577059860522	27.820826792132202	21.72812453662406
34	21.437448072473764	26.048641823047465	29.31325037481965	23.20065972965912
35	21.643540039211903	25.901656032066207	29.43343391288179	23.021370015840102
36	25.514126640792146	26.406132684979678	27.29134254867948	20.788398125548696
37	22.412567579335846	27.844892920439378	28.46478695886516	21.27775254135961
38	22.821191608747128	28.791503774086724	26.70548825408183	21.681816363084323
39	22.359316121834627	28.11703434689588	26.872833165831512	22.650816365437983
40	23.525287675553287	25.870911406050308	27.424559454516782	23.179241463879624
41	21.438213010537314	25.121742834883996	29.01542468184461	24.424619472734076
42	23.01533877341593	25.499239769247605	27.117819291031864	24.367602166304597
43	21.948868009348722	26.35579387625891	27.704497365082574	23.99084074930979
44	21.344360994278265	27.04067822939198	28.72613099034766	22.888829785982097
45	21.782758766778624	28.74681373875835	26.36968044418188	23.100747050281146
46	22.486590047332015	27.486019285853825	27.716295063678153	22.31109560313601
47	22.30229881540515	26.339612494145282	28.578380261302843	22.77970842914673
48	22.618836070242498	26.584274991703367	29.226782952943363	21.570105985110775
49	22.411155385987747	25.740430624824945	29.394274968166805	22.4541390210205
50	21.693878847932666	27.580871605734448	29.103598504016514	21.621651042316373
51	20.938414248089423	27.850806480084543	28.089731942165976	23.121047329660062
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3485.0
1	4629.5
2	5774.0
3	15431.5
4	25089.0
5	18073.0
6	11057.0
7	10897.0
8	10737.0
9	10953.5
10	11170.0
11	11422.0
12	11674.0
13	11444.5
14	11215.0
15	11108.5
16	11002.0
17	10680.0
18	10358.0
19	11473.5
20	12589.0
21	12277.5
22	11966.0
23	13997.5
24	16029.0
25	20739.0
26	30608.5
27	35768.0
28	41111.0
29	46454.0
30	55651.0
31	64848.0
32	69378.5
33	73909.0
34	86581.0
35	99253.0
36	107387.5
37	115522.0
38	130803.0
39	146084.0
40	170616.5
41	195149.0
42	217002.0
43	238855.0
44	237409.5
45	235964.0
46	240964.5
47	245965.0
48	248797.5
49	251630.0
50	251734.0
51	251838.0
52	236085.5
53	220333.0
54	204861.0
55	189389.0
56	183855.5
57	178322.0
58	172544.0
59	166766.0
60	151163.0
61	135560.0
62	122230.0
63	108900.0
64	95047.0
65	81194.0
66	66620.5
67	52047.0
68	43629.0
69	35211.0
70	28135.0
71	21059.0
72	17286.0
73	13513.0
74	11053.0
75	6685.0
76	4777.0
77	3492.0
78	2207.0
79	1628.0
80	1049.0
81	880.0
82	711.0
83	467.5
84	224.0
85	173.5
86	123.0
87	106.5
88	90.0
89	64.0
90	38.0
91	26.5
92	15.0
93	10.5
94	6.0
95	5.0
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3398968.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.35530791003208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.31315940506396	14.285702265224378
2	7.0269213340873025	2.4390876682531704
3	2.0638233629692437	1.0745486980874737
4	0.9925131254105201	0.6890148358499144
5	0.588183453502604	0.5104052471561865
6	0.38370871519899763	0.3995629740024846
7	0.31227912377610206	0.37937902429064896
8	0.23002360375728492	0.3193704375826313
9	0.19638697148269277	0.306752072364074
>10	2.672988132978913	11.74947937997678
>50	1.480052911734581	18.866379517738075
>100	1.7317670133464005	47.129426910047926
>500	0.005461897794403954	0.6425478700717716
>1k	0.0025602645911268537	0.7908691639470821
>5k	0.0	0.0
>10k+	1.7068430607512357E-4	0.4174739354073798
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14093	0.4146258511406992	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	3705	0.10900367405636063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.88413895041083E-5	0.0	0.0	0.016946320177183193	0.0
2	5.88413895041083E-5	0.0	0.0	0.06684381847666704	0.0
3	5.88413895041083E-5	0.0	0.0	0.093440126532524	0.0
4	5.88413895041083E-5	0.0	0.0	0.14925118447717073	0.0
5	5.88413895041083E-5	0.0	0.0	0.26872862586526264	0.0
6	5.88413895041083E-5	0.0	0.0	0.4113307333284691	0.0
7	5.88413895041083E-5	0.0	0.0	0.4871772843992647	0.0
8	5.88413895041083E-5	0.0	0.0	0.6650253841754321	0.0
9	5.88413895041083E-5	0.0	0.0	0.739400900508625	0.0
10	5.88413895041083E-5	0.0	0.0	0.8922708304402983	0.0
11	5.88413895041083E-5	0.0	0.0	1.0976272798096363	0.0
12	5.88413895041083E-5	0.0	0.0	1.2613828667995697	0.0
13	5.88413895041083E-5	0.0	0.0	1.329462354455823	0.0
14	8.826208425616246E-5	0.0	0.0	1.3559409797326718	0.0
15	8.826208425616246E-5	0.0	0.0	1.394040779436582	0.0
16	8.826208425616246E-5	0.0	0.0	1.4798609460283239	0.0
17	8.826208425616246E-5	0.0	0.0	1.5820978602917122	0.0
18	8.826208425616246E-5	0.0	0.0	1.7133141588858736	0.0
19	8.826208425616246E-5	0.0	0.0	1.7856008058916706	0.0
20	8.826208425616246E-5	0.0	0.0	1.8680670132816783	0.0
21	8.826208425616246E-5	0.0	0.0	1.9685975272494476	0.0
22	8.826208425616246E-5	0.0	0.0	2.0811611053708066	0.0
23	8.826208425616246E-5	0.0	0.0	2.1990204085475358	0.0
24	8.826208425616246E-5	0.0	0.0	2.2869588651614254	0.0
25	8.826208425616246E-5	0.0	0.0	2.363776299159039	0.0
26	8.826208425616246E-5	0.0	0.0	2.4358864219963237	0.0
27	8.826208425616246E-5	0.0	0.0	2.5135276354469944	0.0
28	8.826208425616246E-5	0.0	0.0	2.59696472576382	0.0
29	8.826208425616246E-5	0.0	0.0	2.6910815282756415	0.0
30	8.826208425616246E-5	0.0	0.0	2.7982905399521267	0.0
31	8.826208425616246E-5	0.0	0.0	2.891554142316138	0.0
32	8.826208425616246E-5	0.0	0.0	2.9834349720268034	0.0
33	8.826208425616246E-5	0.0	0.0	3.0819354580566807	0.0
34	8.826208425616246E-5	0.0	0.0	3.1800828957495333	0.0
35	8.826208425616246E-5	0.0	0.0	3.301001951180476	0.0
36	8.826208425616246E-5	0.0	0.0	3.4070635557616313	0.0
37	8.826208425616246E-5	0.0	0.0	3.5148315606384055	0.0
38	8.826208425616246E-5	0.0	0.0	3.6375452784492235	0.0
39	8.826208425616246E-5	0.0	0.0	3.8036545210193213	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATCG	25	3.8924234E-5	45.0	20
TACGTTG	110	0.0	40.909092	1
CGGGATA	820	0.0	40.609756	6
CGGTCTA	485	0.0	39.432987	31
CGTTTTT	7960	0.0	39.064068	1
TAGGGAT	4970	0.0	38.797783	5
TAGGGTA	1720	0.0	38.328487	5
ATAGGGA	4695	0.0	38.242813	4
CGTTAGG	395	0.0	38.164555	2
ACGGGTA	360	0.0	38.125	5
CTATGCG	160	0.0	37.96875	1
GGGCGAT	5025	0.0	37.925373	7
ACGGGAT	1425	0.0	37.736843	5
GGCGATT	1540	0.0	37.548702	8
ATAGGGC	1630	0.0	37.546013	4
TACGGGA	1135	0.0	37.466965	4
CGTAAGG	445	0.0	37.415733	2
ATAACGC	310	0.0	37.016132	11
CGAGGGA	2335	0.0	36.90578	4
CGCATCG	245	0.0	36.734695	21
>>END_MODULE