FastQCFastQC Report
Thu 26 May 2016
SRR1547495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547495_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1003144
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34980.34870367564377597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG351.2117198E-745.01
TCGTTAA207.032858E-445.023
TAGCCGT253.8904498E-545.044
CGTTAAT406.8139343E-945.024
GCGATAT453.8380676E-1045.09
TATCGTC207.032858E-445.037
CCGTTTA207.032858E-445.042
TATTACG302.165225E-644.9999961
CGCAACC302.165225E-644.99999616
CGGTCTA1000.042.7531
ACGGGCC950.042.631585
TGTAGCG900.042.51
TTACGAG700.041.7857131
CGAGGGT1050.040.7142874
TCGTTGA1500.040.524
CGTTGAT1500.040.525
CCCCGAT501.0804797E-940.540
TACGGGA4200.039.6428574
AAGGGAC10300.039.5388345
CGGTAGT2450.039.48979612
Produced by FastQC (version 0.11.5)