##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547495_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1003144
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.543387589418867	31.0	31.0	33.0	30.0	34.0
2	31.97784465640028	31.0	31.0	34.0	30.0	34.0
3	32.068899380348185	33.0	31.0	34.0	30.0	34.0
4	35.796863660650914	37.0	35.0	37.0	35.0	37.0
5	35.74008716595025	37.0	35.0	37.0	35.0	37.0
6	35.79780470201686	37.0	35.0	37.0	35.0	37.0
7	36.09066594626494	37.0	35.0	37.0	35.0	37.0
8	36.08651599371576	37.0	35.0	37.0	35.0	37.0
9	37.94502484189707	39.0	38.0	39.0	35.0	39.0
10	37.40905194069845	39.0	37.0	39.0	35.0	39.0
11	37.081634341629915	39.0	37.0	39.0	33.0	39.0
12	36.53458825452776	39.0	35.0	39.0	33.0	39.0
13	36.31145279242063	39.0	35.0	39.0	32.0	39.0
14	37.43133388626159	40.0	35.0	41.0	33.0	41.0
15	37.56130126881086	40.0	35.0	41.0	33.0	41.0
16	37.65034232373418	40.0	35.0	41.0	33.0	41.0
17	37.57765784373928	39.0	35.0	41.0	33.0	41.0
18	37.4736169483145	39.0	35.0	41.0	33.0	41.0
19	37.45513106792245	39.0	35.0	41.0	32.0	41.0
20	37.33341175344716	39.0	35.0	41.0	32.0	41.0
21	37.17582520555374	39.0	35.0	41.0	32.0	41.0
22	37.11419895847455	39.0	35.0	41.0	32.0	41.0
23	37.02595240563668	39.0	35.0	41.0	32.0	41.0
24	36.90018581579514	39.0	35.0	41.0	31.0	41.0
25	36.78863054556474	38.0	35.0	41.0	31.0	41.0
26	36.75566120118348	38.0	35.0	41.0	31.0	41.0
27	36.714212515850164	38.0	35.0	41.0	31.0	41.0
28	36.70144665172697	38.0	35.0	41.0	31.0	41.0
29	36.660050800283905	38.0	35.0	41.0	31.0	41.0
30	36.61212049316948	38.0	35.0	41.0	31.0	41.0
31	36.56535153477467	38.0	35.0	41.0	31.0	41.0
32	36.43638301181087	38.0	35.0	41.0	30.0	41.0
33	36.38190828036653	38.0	35.0	41.0	30.0	41.0
34	36.26625190401378	38.0	35.0	41.0	30.0	41.0
35	36.128846905329645	38.0	35.0	41.0	30.0	41.0
36	35.993305048926175	38.0	35.0	41.0	30.0	41.0
37	35.95026735942198	38.0	35.0	41.0	30.0	41.0
38	35.82992172609316	38.0	35.0	41.0	29.0	41.0
39	35.75421973315895	38.0	35.0	40.0	29.0	41.0
40	35.59088525675277	38.0	35.0	40.0	28.0	41.0
41	35.56180368920115	38.0	35.0	40.0	28.0	41.0
42	35.51038335473272	38.0	34.0	40.0	28.0	41.0
43	35.4103548443693	38.0	34.0	40.0	27.0	41.0
44	35.281745193112855	38.0	34.0	40.0	27.0	41.0
45	35.17331509733398	37.0	34.0	40.0	27.0	41.0
46	35.12303418053639	37.0	34.0	40.0	27.0	41.0
47	35.041641080443085	37.0	34.0	40.0	27.0	41.0
48	34.92181880168749	37.0	34.0	40.0	26.0	41.0
49	34.80064776343177	37.0	34.0	40.0	26.0	41.0
50	34.70148253889771	36.0	34.0	40.0	26.0	41.0
51	34.306265102517685	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	5.0
12	3.0
13	11.0
14	25.0
15	46.0
16	95.0
17	193.0
18	399.0
19	723.0
20	1299.0
21	2039.0
22	3121.0
23	4463.0
24	6413.0
25	8943.0
26	11818.0
27	14383.0
28	16322.0
29	18729.0
30	22450.0
31	27063.0
32	33984.0
33	44533.0
34	76728.0
35	120177.0
36	71444.0
37	93553.0
38	145405.0
39	278702.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.6661536130406	23.872445032816824	29.328690596763774	13.132710757378801
2	30.829571826178494	25.838463869593998	30.040353129760035	13.291611174467475
3	29.85752793218122	25.419580837845814	30.490238689560023	14.232652540412941
4	26.423325065992522	26.9402000111649	30.452756533458807	16.183718389383777
5	23.397737513258317	32.65144386050258	28.757287089390953	15.193531536848148
6	22.309957493639995	41.53471485649119	26.112402606206086	10.042925043662725
7	89.80814319778617	3.27799398690517	5.184798991969249	1.7290638233394207
8	90.15395596245405	2.8787492124759755	5.171939422455799	1.7953554026141811
9	85.72547909372932	4.972865311460768	6.742401888462673	2.5592537063472443
10	44.96253778121586	30.89277312130661	12.823482969543754	11.321206127933776
11	36.1790530571882	22.73103363026644	24.068628232835966	17.021285079709394
12	32.93246034467634	21.947895815555892	27.385001555110733	17.73464228465704
13	23.90544129257614	30.10136132000989	27.193403938018868	18.799793449395104
14	19.327135486031917	33.09046358249663	28.71591715646009	18.866483775011364
15	17.843400349301795	26.335002751349755	38.16042362811321	17.661173271235235
16	21.237130461828013	23.67745807182219	35.972004019363126	19.113407446986674
17	21.222775593533928	22.65686681074701	27.980429529559064	28.139928066159992
18	23.095288413228808	24.97119057682646	31.154450407917505	20.77907060202723
19	27.141666600208943	26.04003014522342	26.066447090347943	20.751856164219692
20	28.663083266211032	24.89782124999003	26.5801320647883	19.85896341901063
21	25.975931670826917	25.712759085435394	29.197104304068013	19.11420493966968
22	25.909540404966787	23.031289625417685	27.461062419752295	23.59810754986323
23	22.343850932667692	28.170731221041045	27.27743972949048	22.207978116800778
24	21.04782563619979	25.866376113499157	33.372975365451026	19.71282288485003
25	20.783556498369126	26.772826234319304	30.942815787165152	21.50080148014642
26	20.803194755688118	30.06796631390907	26.808713405054508	22.320125525348306
27	20.709688738605823	29.587875718740282	29.51301109312322	20.189424449530676
28	18.618662923767673	27.475417288046383	34.017150080148014	19.88876970803793
29	20.073488950738877	25.70099606836107	32.84872361296085	21.376791367939198
30	20.55936136785945	27.374036030719417	31.03442775912531	21.032174842295824
31	26.608542741620344	26.06973674766534	26.49958530380484	20.822135206909476
32	26.06096432815229	26.381955133061652	27.623551553914496	19.93352898487156
33	24.6003564792293	26.251864139146523	27.8529303868637	21.294848994760475
34	20.94993340936097	26.498787811121833	29.141778249184565	23.409500530332632
35	21.971521536289902	25.948916606190135	29.931495378529902	22.148066478990057
36	27.08982957581364	25.85939805252287	27.208755672166706	19.84201669949678
37	21.697283739921687	28.619918974743406	28.964734873557536	20.71806241177737
38	22.316337435104032	30.05351175902961	25.908643225698402	21.721507580167952
39	21.9282575582369	28.16554751860152	27.743873262462813	22.162321660698765
40	24.019682119416554	26.284162592808215	27.613582895376936	22.0825723923983
41	20.998082030097372	24.485716906047386	29.19840022967789	25.317800834177344
42	22.731631749778696	25.944031963506735	26.861048862376684	24.46328742433788
43	22.584394663178966	26.078608853763768	27.447804103897344	23.889192379159923
44	20.75534519470784	28.049412646638967	28.903726683307678	22.291515475345513
45	20.411526161747464	30.484556554193613	26.032753024491	23.07116425956792
46	22.510925649757162	28.62899045401258	27.06301388434761	21.797070011882642
47	22.35970109974241	26.805722807493243	28.272610911294887	22.56196518146946
48	23.15071415469763	25.866475800084533	29.43794709433541	21.544862950882425
49	21.968630625314013	25.210936814654726	30.307911924908087	22.512520635123174
50	20.68317210689592	28.850494046717124	28.589614252789232	21.87671959359773
51	20.203181198312507	29.217041621143125	27.49595272463375	23.083824455910616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	860.0
1	1268.5
2	1677.0
3	4093.0
4	6509.0
5	4902.0
6	3295.0
7	3180.0
8	3065.0
9	3095.0
10	3125.0
11	3125.0
12	3125.0
13	3054.0
14	2983.0
15	3058.5
16	3134.0
17	3099.5
18	3065.0
19	3102.0
20	3139.0
21	3325.5
22	3512.0
23	4228.5
24	4945.0
25	5807.0
26	8313.5
27	9958.0
28	12339.5
29	14721.0
30	17099.0
31	19477.0
32	20574.5
33	21672.0
34	25879.0
35	30086.0
36	32058.5
37	34031.0
38	38852.0
39	43673.0
40	51705.0
41	59737.0
42	68457.0
43	77177.0
44	79164.5
45	81152.0
46	80890.0
47	80628.0
48	79363.0
49	78098.0
50	73877.0
51	69656.0
52	66291.0
53	62926.0
54	57354.0
55	51782.0
56	50529.5
57	49277.0
58	46014.5
59	42752.0
60	39758.0
61	36764.0
62	32943.0
63	29122.0
64	24274.5
65	19427.0
66	17439.0
67	15451.0
68	12851.0
69	10251.0
70	8333.0
71	6415.0
72	5181.5
73	3948.0
74	3376.5
75	2134.5
76	1464.0
77	1156.5
78	849.0
79	561.5
80	274.0
81	276.0
82	278.0
83	192.0
84	106.0
85	72.0
86	38.0
87	26.5
88	15.0
89	13.0
90	11.0
91	10.0
92	9.0
93	7.0
94	5.0
95	4.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1003144.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.78629753753116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.331374219083	15.894604718975359
2	5.367581500627376	2.124091292567225
3	1.6962314139546546	1.0068641834704195
4	0.94706624056657	0.7495573769440487
5	0.6378073250345103	0.6309922752374832
6	0.5097217532836675	0.6051303763093618
7	0.43674031478493885	0.6049031670478874
8	0.3718389689687306	0.5885853180851296
9	0.33549796905661955	0.5974436375092527
>10	6.50199069026899	33.524336921480106
>50	2.5272802221801856	34.60955200269431
>100	0.3338299591935372	8.35961165400257
>500	0.002532852497674789	0.3537171268056913
>1k	5.065704995349578E-4	0.3506099488711556
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3498	0.34870367564377597	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.968658537557918E-5	0.0	0.0	0.028610050002791224	0.0
2	9.968658537557918E-5	0.0	0.0	0.09490162927755137	0.0
3	9.968658537557918E-5	0.0	0.0	0.13338065123252493	0.0
4	9.968658537557918E-5	0.0	0.0	0.24343464148716434	0.0
5	9.968658537557918E-5	0.0	0.0	0.424963913456094	0.0
6	9.968658537557918E-5	0.0	0.0	0.6184555756700932	0.0
7	9.968658537557918E-5	0.0	0.0	0.7120612793377621	0.0
8	9.968658537557918E-5	0.0	0.0	0.9358576635059374	0.0
9	9.968658537557918E-5	0.0	0.0	0.9976633464387964	0.0
10	9.968658537557918E-5	0.0	0.0	1.1226703244997727	0.0
11	9.968658537557918E-5	0.0	0.0	1.2957262367117781	0.0
12	9.968658537557918E-5	0.0	0.0	1.442664263555382	0.0
13	9.968658537557918E-5	0.0	0.0	1.4952987806336877	0.0
14	9.968658537557918E-5	0.0	0.0	1.5140398586842965	0.0
15	9.968658537557918E-5	0.0	0.0	1.5477339245412423	0.0
16	9.968658537557918E-5	0.0	0.0	1.6275828794270812	0.0
17	9.968658537557918E-5	0.0	0.0	1.7220857623631303	0.0
18	9.968658537557918E-5	0.0	0.0	1.8473918001802334	0.0
19	9.968658537557918E-5	0.0	0.0	1.9152783648210028	0.0
20	9.968658537557918E-5	0.0	0.0	1.9893454977550582	0.0
21	9.968658537557918E-5	0.0	0.0	2.0866396050816234	0.0
22	9.968658537557918E-5	0.0	0.0	2.1979895209461455	0.0
23	9.968658537557918E-5	0.0	0.0	2.315619691689329	0.0
24	9.968658537557918E-5	0.0	0.0	2.4010510953562	0.0
25	9.968658537557918E-5	0.0	0.0	2.478009139266147	0.0
26	9.968658537557918E-5	0.0	0.0	2.554867496590719	0.0
27	9.968658537557918E-5	0.0	0.0	2.6352148844034358	0.0
28	9.968658537557918E-5	0.0	0.0	2.7129704209963874	0.0
29	9.968658537557918E-5	0.0	0.0	2.8003955563707703	0.0
30	9.968658537557918E-5	0.0	0.0	2.908057068576396	0.0
31	9.968658537557918E-5	0.0	0.0	3.0026596380978203	0.0
32	9.968658537557918E-5	0.0	0.0	3.1002528051805123	0.0
33	9.968658537557918E-5	0.0	0.0	3.2059205856786264	0.0
34	1.9937317075115836E-4	0.0	0.0	3.303812812517445	0.0
35	1.9937317075115836E-4	0.0	0.0	3.4292185369199237	0.0
36	1.9937317075115836E-4	0.0	0.0	3.5384750344915585	0.0
37	1.9937317075115836E-4	0.0	0.0	3.6446412479165504	0.0
38	1.9937317075115836E-4	0.0	0.0	3.760576746708349	0.0
39	1.9937317075115836E-4	0.0	0.0	3.9063185345274456	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	35	1.2117198E-7	45.0	1
TCGTTAA	20	7.032858E-4	45.0	23
TAGCCGT	25	3.8904498E-5	45.0	44
CGTTAAT	40	6.8139343E-9	45.0	24
GCGATAT	45	3.8380676E-10	45.0	9
TATCGTC	20	7.032858E-4	45.0	37
CCGTTTA	20	7.032858E-4	45.0	42
TATTACG	30	2.165225E-6	44.999996	1
CGCAACC	30	2.165225E-6	44.999996	16
CGGTCTA	100	0.0	42.75	31
ACGGGCC	95	0.0	42.63158	5
TGTAGCG	90	0.0	42.5	1
TTACGAG	70	0.0	41.785713	1
CGAGGGT	105	0.0	40.714287	4
TCGTTGA	150	0.0	40.5	24
CGTTGAT	150	0.0	40.5	25
CCCCGAT	50	1.0804797E-9	40.5	40
TACGGGA	420	0.0	39.642857	4
AAGGGAC	1030	0.0	39.538834	5
CGGTAGT	245	0.0	39.489796	12
>>END_MODULE