FastQCFastQC Report
Thu 26 May 2016
SRR1547494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547494_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences278230
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10650.38277683930561046No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG207.024059E-445.0000042
AGGCATA207.024059E-445.00000437
TTGTTAG406.7830115E-945.0000041
TAATGCG207.024059E-445.0000041
TAGACGG351.2074997E-745.02
ATAGCGG253.8831597E-545.02
CACGACG253.8831597E-545.026
ACTAACC253.8831597E-545.014
ACGTCGC253.8831597E-545.020
CTAGGCG253.8831597E-545.01
CTATGCG302.1595515E-644.9999961
GTTAGCG302.1595515E-644.9999961
CGTTAGG600.044.9999962
TATTGCG302.1595515E-644.9999961
AGGGCGA1900.042.631586
CTCGTCC700.041.78571337
TATTAGG1250.041.42
CGAGGGA1800.041.254
GTTACGG556.002665E-1140.9090922
CGAGATA556.002665E-1140.90909219
Produced by FastQC (version 0.11.5)