FastQCFastQC Report
Thu 26 May 2016
SRR1547493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547493_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4636335
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203110.4380830979642325No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG207.0355023E-445.00000434
CGGTCTA5750.042.65217231
TAACGCC1750.042.4285712
CATACGA12850.042.02334618
TCGATAG2350.041.170211
GCGTAAG2850.041.052631
ACGGGTA5900.040.8050845
CGTACTC501.0822987E-940.50000436
CGTAAGG7950.040.188682
CGACGGT6050.040.16528728
ACATACG13250.040.07547417
TACGGGA17650.039.773374
CGTTTTT104500.039.7679441
TAACGCG850.039.7058831
CGGGTAT5500.039.681826
ATACGAA13450.039.47955319
GCGATCG403.4612458E-739.3750049
AGGGCGA33600.038.571436
TAGGGAT61800.038.5558245
TATTACG1700.038.382351
Produced by FastQC (version 0.11.5)