FastQCFastQC Report
Thu 26 May 2016
SRR1547492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547492_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences7197320
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT923821.2835611032995615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT347400.042.700781
ACCGTTC17350.041.88760845
CGGTCTA9200.041.0869631
ACGTAAG1700.039.7058831
ACGTTCA1600.039.3759
TCACGAC9750.039.23076625
ACGGGAT36800.038.702455
AAGGGAT128900.038.681155
TACGGGA27650.038.571434
CGTAAGG12250.038.571432
TAGGGAT124250.038.2997975
ATAGGGA104100.037.780984
ACGGGAC29350.037.717215
AGGGATT131800.037.6593326
TCGTCCC38250.037.58823438
GGGCGAT93250.037.471857
TATGGGA77300.037.4320834
AGGGATG152800.037.1220556
GGCGATA19200.037.0312468
AACGGGA30350.036.845144
Produced by FastQC (version 0.11.5)