FastQCFastQC Report
Thu 26 May 2016
SRR1547491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547491_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4392594
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191840.4367351045874032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA4300.043.95348731
TCGGTAA603.6379788E-1241.24999634
CGTTTTT99900.040.518021
CGTAAGG9550.039.5811542
AGTCGGC1650.039.54545230
TACGGGA17200.039.2441864
ATAGCGG10400.039.1586572
CGACGGT4850.038.96907428
GGACCGA24600.038.9634138
ACGGGAT22650.038.7417225
CGTTAGG11500.038.7391322
AAGGGAT86250.038.6608665
GGGCGAT64750.038.432437
ACGTAAG3000.038.251
CGCATCG1650.038.18181621
AGGGATT102750.038.167886
TTAGGGA67750.038.1586724
TAAGGGA67050.038.154364
TAGGGAC52050.038.0835725
ACGATAA659.094947E-1238.07692719
Produced by FastQC (version 0.11.5)