FastQCFastQC Report
Thu 26 May 2016
SRR1547490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547490_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3595195
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144790.4027319797674396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA453.8562575E-1045.020
CGGCATA253.8924685E-545.017
CCGCAAT556.184564E-1140.90909233
CCCGATA556.184564E-1140.90909232
TACGGGA15800.040.158234
CGTTAGG4650.039.677422
CGGTCTA4600.039.61956831
TAGACGG4150.039.5783122
CGTTTTT81900.038.4065971
GGGCGAT45650.038.2968257
ACGGGAT14250.037.736845
TAGGGAC32750.037.6488535
TAACGGG8650.037.196533
CACGACG4900.037.1938826
TAGGGAT55750.037.008975
ATAGGGC11950.036.9037634
TAAGGGA41600.036.887024
GTAGGGA48250.036.839384
AGGGATG62000.036.5443536
AGGGATA37000.036.4256746
Produced by FastQC (version 0.11.5)