##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547490_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3595195
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.561746163977197	31.0	31.0	33.0	30.0	34.0
2	31.990436680068814	31.0	31.0	34.0	30.0	34.0
3	32.068903912027025	33.0	31.0	34.0	30.0	34.0
4	35.796799339117904	37.0	35.0	37.0	35.0	37.0
5	35.750124263078916	37.0	35.0	37.0	35.0	37.0
6	35.80495522495998	37.0	35.0	37.0	35.0	37.0
7	36.10334599374999	37.0	35.0	37.0	35.0	37.0
8	36.094135644937204	37.0	35.0	37.0	35.0	37.0
9	37.899110340329244	39.0	38.0	39.0	35.0	39.0
10	37.40740015492901	39.0	37.0	39.0	35.0	39.0
11	37.11923720410159	39.0	37.0	39.0	33.0	39.0
12	36.70422411023603	39.0	35.0	39.0	33.0	39.0
13	36.55885397036879	39.0	35.0	39.0	32.0	39.0
14	37.66808587573136	40.0	36.0	41.0	33.0	41.0
15	37.762433748378044	40.0	36.0	41.0	33.0	41.0
16	37.82443316704657	40.0	36.0	41.0	33.0	41.0
17	37.732242896421475	40.0	36.0	41.0	33.0	41.0
18	37.60836171612388	39.0	36.0	41.0	33.0	41.0
19	37.59919475855969	39.0	36.0	41.0	33.0	41.0
20	37.50303697017825	39.0	35.0	41.0	32.0	41.0
21	37.39680045171402	39.0	35.0	41.0	32.0	41.0
22	37.33254691331068	39.0	35.0	41.0	32.0	41.0
23	37.22453330069718	39.0	35.0	41.0	32.0	41.0
24	37.104190454203454	39.0	35.0	41.0	32.0	41.0
25	36.975949844167005	39.0	35.0	41.0	31.0	41.0
26	36.93123154655033	39.0	35.0	41.0	31.0	41.0
27	36.87905051047301	39.0	35.0	41.0	31.0	41.0
28	36.84037611311765	39.0	35.0	41.0	31.0	41.0
29	36.75924616050033	39.0	35.0	41.0	31.0	41.0
30	36.690229319967344	39.0	35.0	41.0	31.0	41.0
31	36.62361207111158	39.0	35.0	41.0	30.0	41.0
32	36.51865420373582	39.0	35.0	41.0	30.0	41.0
33	36.42278012736444	39.0	35.0	41.0	30.0	41.0
34	36.27525266362464	39.0	35.0	41.0	30.0	41.0
35	36.107578587531414	39.0	35.0	41.0	29.0	41.0
36	36.002675793663485	39.0	35.0	41.0	29.0	41.0
37	35.97518326544179	39.0	35.0	41.0	29.0	41.0
38	35.8180310108353	38.0	35.0	41.0	28.0	41.0
39	35.71165708675051	38.0	35.0	41.0	27.0	41.0
40	35.55574982719992	38.0	35.0	41.0	27.0	41.0
41	35.47421071736026	38.0	34.0	40.0	26.0	41.0
42	35.454302200576045	38.0	34.0	40.0	27.0	41.0
43	35.355552897687055	38.0	34.0	40.0	26.0	41.0
44	35.204658996243595	38.0	34.0	40.0	26.0	41.0
45	35.08553360805186	38.0	34.0	40.0	26.0	41.0
46	35.013254635701266	38.0	34.0	40.0	26.0	41.0
47	34.937761373166126	37.0	34.0	40.0	25.0	41.0
48	34.794320196818255	37.0	34.0	40.0	24.0	41.0
49	34.67137721319706	37.0	34.0	40.0	24.0	41.0
50	34.56470316630948	37.0	34.0	40.0	24.0	41.0
51	34.18374107663145	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	10.0
11	14.0
12	27.0
13	38.0
14	102.0
15	197.0
16	380.0
17	774.0
18	1681.0
19	3195.0
20	5390.0
21	8738.0
22	12947.0
23	17956.0
24	25494.0
25	35290.0
26	46762.0
27	54446.0
28	60454.0
29	67857.0
30	78404.0
31	95110.0
32	118220.0
33	152430.0
34	250586.0
35	349734.0
36	258808.0
37	349977.0
38	549548.0
39	1050300.0
40	321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.845815039239874	23.501423427658306	28.141310832931175	13.511450700170643
2	31.594586663588487	25.734765429969723	29.605737658179876	13.064910248261915
3	30.563460396445812	25.89795546555889	29.945802661608063	13.592781476387234
4	26.88691433983414	27.73777222097828	30.051471477903146	15.323841961284437
5	23.93792269960322	32.053699451629186	29.023210145764	14.985167703003594
6	22.824631209155555	41.16928289007968	25.856177481332722	10.149908419432048
7	88.24486571660229	3.8374830850621455	5.998200375779339	1.9194508225562175
8	88.46785779352719	3.452191049442381	6.174825009491835	1.9051261475385897
9	82.86932975819114	6.2662804103810785	7.63886242609928	3.225527405328501
10	41.28474255221205	34.514734249463515	13.736417635204766	10.464105563119663
11	32.93946503597162	23.76257755142628	25.79670921883236	17.50124819376974
12	30.63675266571076	23.154960996552344	29.105764777710245	17.102521560026645
13	25.391362638187914	26.11722034548891	29.72200395249771	18.769413063825464
14	20.529929530943384	29.84171929478095	30.713021129591024	18.91533004468464
15	20.013657117346902	26.673879998164217	35.59153258724492	17.72093029724396
16	24.691039011792128	24.382766442432192	32.9070328591356	18.019161686640082
17	24.560003004009516	24.469548939626364	28.10912899022167	22.86131906614245
18	24.89717525753123	25.54378830633665	30.505021285354477	19.054015150777634
19	27.199943257597987	26.798490763366107	26.728786616581296	19.27277936245461
20	27.53867314568473	26.926411502018667	27.05422098105944	18.480694371237167
21	27.08008884080001	25.719550678057796	29.12626436118208	18.074096119960114
22	25.67184811950395	22.91533560766523	29.02365518421115	22.389161088619673
23	23.292088468080312	26.451026995754056	29.222726444601754	21.034158091563878
24	22.31606352367535	26.137302705416538	31.532475985308167	20.014157785599945
25	23.757821203022367	26.61830582207641	29.102955472512615	20.520917502388606
26	22.31837216061994	28.064096662350718	27.482069818187888	22.135461358841454
27	20.93102043143696	28.372091082681187	30.177306098834695	20.519582387047155
28	20.078271136892436	27.54871432564854	31.8240039830941	20.549010554364923
29	21.40559830551611	26.75660151952815	31.167989497092645	20.669810677863094
30	22.661524618275227	26.484961177349213	30.010249791735916	20.84326441263965
31	25.696353049000127	25.870001488097305	28.03625394450092	20.397391518401644
32	26.08562261574129	26.137636484251896	27.66984822798207	20.106892672024745
33	25.090015979661747	26.035778309660536	27.83640386682781	21.037801843849916
34	22.031350177111396	26.869641285104144	29.74823340597659	21.350775131807872
35	22.806662781851887	27.27181696681265	29.48961043837678	20.431909812958686
36	25.555665269894956	27.86850226482847	27.290119172951677	19.285713292324893
37	23.149147681836453	29.220974105716103	26.893144878094233	20.736733334353215
38	22.95497184436449	29.633580376029673	26.680277425841993	20.731170353763844
39	22.517582495525275	27.760719515909425	27.320743381096158	22.40095460746914
40	23.604199494046917	26.19958027311453	28.158111034311073	22.03810919852748
41	20.80615933210855	26.538588310230736	28.868308951252992	23.78694340640772
42	22.228057170751516	27.418596209663175	27.288339019163075	23.06500760042223
43	22.495358388070745	26.29326086623952	27.79932660119966	23.412054144490078
44	21.63793618983115	27.93842893083685	29.140895000132122	21.282739879199877
45	21.679825433669105	28.60762768083511	27.26305527238439	22.449491613111388
46	22.260044309140394	28.768759413606215	27.247701445957727	21.72349483129566
47	22.047427191014673	27.731096644271037	28.935315052451955	21.286161112262338
48	22.5120751447418	27.486937426203582	28.970417459970875	21.030569969083736
49	22.637687246449776	26.666815012815714	29.052471423664084	21.643026317070422
50	21.29292013367842	28.675802007957845	28.711154749603292	21.32012310876044
51	21.20861316284652	28.292234496320784	28.32157921892971	22.177573121902984
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2748.0
1	4832.0
2	6916.0
3	17448.5
4	27981.0
5	20710.5
6	13440.0
7	13082.5
8	12725.0
9	12965.5
10	13206.0
11	13107.0
12	13008.0
13	12947.5
14	12887.0
15	12440.5
16	11994.0
17	12373.0
18	12752.0
19	13206.5
20	13661.0
21	15789.0
22	17917.0
23	19402.5
24	20888.0
25	24730.0
26	35489.5
27	42407.0
28	48963.5
29	55520.0
30	63576.0
31	71632.0
32	82107.0
33	92582.0
34	102952.0
35	113322.0
36	122732.0
37	132142.0
38	152433.0
39	172724.0
40	186626.5
41	200529.0
42	219738.0
43	238947.0
44	242066.5
45	245186.0
46	251365.0
47	257544.0
48	259557.0
49	261570.0
50	256427.0
51	251284.0
52	235389.5
53	219495.0
54	214253.0
55	209011.0
56	195837.0
57	182663.0
58	176372.5
59	170082.0
60	154733.5
61	139385.0
62	124543.0
63	109701.0
64	94210.5
65	78720.0
66	67232.0
67	55744.0
68	45558.0
69	35372.0
70	27477.5
71	19583.0
72	16653.0
73	13723.0
74	10930.5
75	6327.5
76	4517.0
77	3630.0
78	2743.0
79	1981.5
80	1220.0
81	864.0
82	508.0
83	335.5
84	163.0
85	143.5
86	124.0
87	108.0
88	92.0
89	66.0
90	40.0
91	43.0
92	46.0
93	38.5
94	31.0
95	19.5
96	8.0
97	4.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3595195.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.48107245682214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.34609214775946	14.394980033711358
2	6.716193060022431	2.348125150325162
3	2.0027243741882432	1.050293096886854
4	0.9874717707617954	0.6904826229501363
5	0.6239788340043411	0.545390960437664
6	0.469218444457759	0.4921464975386076
7	0.34640707844095975	0.42388970664477404
8	0.2652270862035208	0.3709163129148447
9	0.23141414151351689	0.36408306377979727
>10	2.884426992837915	12.610700825238805
>50	1.4286965863930297	18.347517485985158
>100	1.6918963127272435	46.86053461364099
>500	0.0035274296198470187	0.41967449260840034
>1k	0.0025654033598887407	0.675436847851281
>5k	0.0	0.0
>10k+	1.603377099930463E-4	0.4058282894862104
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14479	0.4027319797674396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1125961178739958E-4	0.0	0.0	0.015047862494245792	0.0
2	1.1125961178739958E-4	0.0	0.0	0.044865438453268874	0.0
3	1.1125961178739958E-4	0.0	0.0	0.06806306751094168	0.0
4	1.1125961178739958E-4	0.0	0.0	0.12110608743058443	0.0
5	1.1125961178739958E-4	0.0	0.0	0.22677490372566717	0.0
6	1.1125961178739958E-4	0.0	0.0	0.35241482033658816	0.0
7	1.1125961178739958E-4	0.0	0.0	0.41830832541767554	0.0
8	1.1125961178739958E-4	0.0	0.0	0.5539894219924093	0.0
9	1.1125961178739958E-4	0.0	0.0	0.614459020998861	0.0
10	1.1125961178739958E-4	0.0	0.0	0.7214629526353925	0.0
11	1.1125961178739958E-4	0.0	0.0	0.8593136116399805	0.0
12	1.1125961178739958E-4	0.0	0.0	0.9792236582438505	0.0
13	1.1125961178739958E-4	0.0	0.0	1.0277884787890503	0.0
14	1.1125961178739958E-4	0.0	0.0	1.0508192184290421	0.0
15	1.1125961178739958E-4	0.0	0.0	1.0825838375943446	0.0
16	1.1125961178739958E-4	0.0	0.0	1.1448057754864478	0.0
17	1.1125961178739958E-4	0.0	0.0	1.217625191401301	0.0
18	1.1125961178739958E-4	0.0	0.0	1.3060487678693367	0.0
19	1.1125961178739958E-4	0.0	0.0	1.3623183165308141	0.0
20	1.1125961178739958E-4	0.0	0.0	1.427933672582433	0.0
21	1.1125961178739958E-4	0.0	0.0	1.5041186917538547	0.0
22	1.1125961178739958E-4	0.0	0.0	1.592848232154306	0.0
23	1.1125961178739958E-4	0.0	0.0	1.6809658446899265	0.0
24	1.1125961178739958E-4	0.0	0.0	1.746831534868067	0.0
25	1.1125961178739958E-4	0.0	0.0	1.8071064295538908	0.0
26	1.1125961178739958E-4	0.0	0.0	1.8669362857925649	0.0
27	1.1125961178739958E-4	0.0	0.0	1.932301307717662	0.0
28	1.1125961178739958E-4	0.0	0.0	1.9990014449842082	0.0
29	1.1125961178739958E-4	0.0	0.0	2.0751030194467894	0.0
30	1.1125961178739958E-4	0.0	0.0	2.17893605214738	0.0
31	1.1125961178739958E-4	0.0	0.0	2.265162251282615	0.0
32	1.1125961178739958E-4	0.0	0.0	2.3482731812878024	0.0
33	1.1125961178739958E-4	0.0	0.0	2.433303339596322	0.0
34	1.1125961178739958E-4	0.0	0.0	2.521949435287933	0.0
35	1.1125961178739958E-4	0.0	0.0	2.6242248334235	0.0
36	1.1125961178739958E-4	0.0	0.0	2.7076695422640498	0.0
37	1.1125961178739958E-4	0.0	0.0	2.797873272520684	0.0
38	1.1125961178739958E-4	0.0	0.0	2.918979359951268	0.0
39	1.1125961178739958E-4	0.0	0.0	3.1352124154600793	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	45	3.8562575E-10	45.0	20
CGGCATA	25	3.8924685E-5	45.0	17
CCGCAAT	55	6.184564E-11	40.909092	33
CCCGATA	55	6.184564E-11	40.909092	32
TACGGGA	1580	0.0	40.15823	4
CGTTAGG	465	0.0	39.67742	2
CGGTCTA	460	0.0	39.619568	31
TAGACGG	415	0.0	39.578312	2
CGTTTTT	8190	0.0	38.406597	1
GGGCGAT	4565	0.0	38.296825	7
ACGGGAT	1425	0.0	37.73684	5
TAGGGAC	3275	0.0	37.648853	5
TAACGGG	865	0.0	37.19653	3
CACGACG	490	0.0	37.19388	26
TAGGGAT	5575	0.0	37.00897	5
ATAGGGC	1195	0.0	36.903763	4
TAAGGGA	4160	0.0	36.88702	4
GTAGGGA	4825	0.0	36.83938	4
AGGGATG	6200	0.0	36.544353	6
AGGGATA	3700	0.0	36.425674	6
>>END_MODULE