FastQCFastQC Report
Thu 26 May 2016
SRR1547489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547489_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2516550
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161370.641235024140192No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC60960.24223639506467187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28640.11380660030597446No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25540.10148814845721324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCATA600.045.00000430
CGTAAGA302.1665346E-645.00000410
GTATACG1200.045.0000041
CATCGAA302.1665346E-645.00000435
TCGAAGT302.1665346E-645.00000437
TACGTGC302.1665346E-645.00000441
TATCACG207.0348877E-445.01
AGGTAAC453.8562575E-1045.033
AACCGAT253.8921335E-545.024
CGAACTA406.8212103E-945.022
CGTATTA453.8562575E-1045.031
GATCGAC253.8921335E-545.09
ACGCATA207.0348877E-445.022
ACGTTAG800.045.01
ACGTTAC207.0348877E-445.045
GCGAACT207.0348877E-445.09
GCGAAAC406.8212103E-945.023
CACGCGT207.0348877E-445.038
GCACGAT351.212702E-745.013
GTCTAGA253.8921335E-545.037