##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547489_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2516550
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60675766426258	31.0	31.0	33.0	30.0	34.0
2	32.01645149112873	31.0	31.0	34.0	30.0	34.0
3	32.03772585484095	33.0	31.0	34.0	30.0	34.0
4	35.774095090500886	37.0	35.0	37.0	35.0	37.0
5	35.7137907055294	37.0	35.0	37.0	33.0	37.0
6	35.77048498937037	37.0	35.0	37.0	35.0	37.0
7	36.113536389104134	37.0	35.0	37.0	35.0	37.0
8	36.12906797003835	37.0	35.0	37.0	35.0	37.0
9	37.99980807057281	39.0	39.0	39.0	35.0	39.0
10	37.495067850827525	39.0	37.0	39.0	35.0	39.0
11	37.10088494168604	39.0	37.0	39.0	33.0	39.0
12	36.07898352903777	37.0	35.0	39.0	33.0	39.0
13	35.60921579146053	37.0	35.0	39.0	31.0	39.0
14	36.54116826607856	38.0	35.0	41.0	31.0	41.0
15	36.860240805865175	38.0	35.0	41.0	32.0	41.0
16	37.02850450020862	38.0	35.0	41.0	33.0	41.0
17	36.992570781426956	38.0	35.0	41.0	32.0	41.0
18	36.888181438874646	38.0	35.0	41.0	32.0	41.0
19	36.8273978263893	38.0	35.0	41.0	32.0	41.0
20	36.639010152788536	37.0	35.0	41.0	32.0	41.0
21	36.486852238183225	37.0	35.0	40.0	32.0	41.0
22	36.38384335697681	37.0	35.0	40.0	31.0	41.0
23	36.37187419284338	37.0	35.0	40.0	31.0	41.0
24	36.22232659792176	37.0	35.0	40.0	31.0	41.0
25	36.09472492102283	36.0	35.0	40.0	31.0	41.0
26	36.03131509407721	36.0	35.0	40.0	31.0	41.0
27	35.969009159365	36.0	35.0	40.0	31.0	41.0
28	35.98531958435159	36.0	35.0	40.0	31.0	41.0
29	35.978751067930304	36.0	35.0	40.0	31.0	41.0
30	35.95941666169955	36.0	35.0	40.0	31.0	41.0
31	35.754933142596016	36.0	35.0	40.0	30.0	41.0
32	35.56576543283464	36.0	34.0	40.0	30.0	41.0
33	35.44309590510819	36.0	34.0	40.0	30.0	41.0
34	35.32177385706622	36.0	34.0	40.0	29.0	41.0
35	35.12506010212394	36.0	34.0	40.0	29.0	41.0
36	34.92672190101528	36.0	34.0	40.0	27.0	41.0
37	34.83763445987562	36.0	34.0	40.0	27.0	41.0
38	34.70112137648765	35.0	34.0	40.0	26.0	41.0
39	34.674125687945796	36.0	34.0	40.0	26.0	41.0
40	34.465163418171706	36.0	34.0	40.0	24.0	41.0
41	34.41344896783295	35.0	34.0	40.0	24.0	41.0
42	34.36351791142636	35.0	34.0	40.0	24.0	41.0
43	34.23778267866722	35.0	33.0	40.0	24.0	41.0
44	34.09013013848324	35.0	33.0	40.0	23.0	41.0
45	33.96367248812859	35.0	33.0	40.0	23.0	41.0
46	33.92418469730385	35.0	33.0	40.0	23.0	41.0
47	33.85789433947269	35.0	33.0	40.0	23.0	41.0
48	33.74812699926487	35.0	33.0	39.0	23.0	41.0
49	33.69509606405595	35.0	33.0	39.0	23.0	41.0
50	33.54239415072222	35.0	33.0	39.0	23.0	41.0
51	33.15443841767499	35.0	33.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	10.0
12	9.0
13	16.0
14	36.0
15	87.0
16	242.0
17	525.0
18	1248.0
19	2457.0
20	4278.0
21	6841.0
22	10441.0
23	15160.0
24	21320.0
25	30259.0
26	39734.0
27	45924.0
28	49877.0
29	54969.0
30	63866.0
31	76916.0
32	95941.0
33	127590.0
34	243425.0
35	451867.0
36	156141.0
37	191306.0
38	289432.0
39	536485.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.947408157994076	24.087619955891995	29.256044982217716	13.708926903896208
2	30.388627287357693	26.05571119190956	29.95469988674972	13.600961633983033
3	28.622479187776918	25.7048339989271	31.781367348155214	13.891319465140766
4	25.741789354473386	27.157179471896047	31.065188452444815	16.03584272118575
5	23.249289702171623	32.442311895253425	30.074427291331386	14.233971111243568
6	22.03099481432914	40.77165166597127	27.279807673203393	9.917545846496195
7	88.2391766505732	3.8972402694164634	6.369513818521389	1.494069261488943
8	89.18129979535475	2.9641771472849734	5.996979992449981	1.8575430649102938
9	85.5265740795931	4.797440940970773	7.280960044505375	2.3950249349307584
10	53.69271423178558	24.590332002145797	12.412469452226262	9.304484313842364
11	48.0050068546224	19.766823627585385	18.732669726411157	13.495499791381057
12	42.350916929923905	19.951083825077983	23.47145894180525	14.226540303192865
13	22.777890365778546	39.5848284357553	23.741153563410226	13.89612763505593
14	15.67034233375057	42.63181736901711	27.155589994238145	14.542250302994178
15	14.357314577496972	24.128310583934354	47.733961177008204	13.78041366156047
16	16.87119270429755	19.463034710218356	45.80369950924877	17.862073076235323
17	18.628161570403925	18.626214460272994	27.2179769923109	35.527646977012175
18	23.16731239196519	23.22866622956031	33.53332935963919	20.07069201883531
19	29.227275436609645	25.243766267310402	25.18471717231925	20.344241123760703
20	32.31022630188154	23.79388448471121	24.867457431801473	19.02843178160577
21	23.967932288251774	26.82132284278079	28.37134171782798	20.839403151139457
22	24.150006953964752	22.711887306034054	27.150821561264433	25.987284178736765
23	20.431463710238223	29.553833621426158	24.603365718940612	25.411336949395004
24	20.940017086884822	24.141185352963383	37.84812540978721	17.070672150364587
25	18.16200751028193	24.535534759889533	35.51667958117264	21.7857781486559
26	17.477061850549365	34.467584590014106	26.156960918718084	21.898392640718445
27	18.606703622022213	33.095468001827896	29.97258151040114	18.325246865748742
28	14.949077109534878	28.706165186465597	38.93036101011305	17.414396693886474
29	15.734279072539787	25.722040094573924	37.8457014563589	20.69797937652739
30	19.028709940195903	30.036518249190358	32.84246289563092	18.092308914982812
31	30.69507857980171	26.65426874093501	24.45284218473704	18.197810494526237
32	29.8857562933381	26.868331644513322	25.532256462220104	17.713655599928472
33	27.64471200651686	27.96733623413006	24.339870060201466	20.048081699151616
34	19.789910790566452	28.169160159742507	28.427728437742143	23.613200611948898
35	20.01037134171783	26.23361347877054	30.870397965468598	22.885617214043037
36	31.76988337207685	23.17069003198824	27.35109574616042	17.70833084977449
37	20.821601001370922	30.199002602769664	29.776519441298603	19.202876954560807
38	20.854741610538234	32.1884325763446	24.22109634221454	22.735729470902623
39	21.131270986072202	30.42355605889015	27.65106991714848	20.794103037889176
40	24.752180564661938	26.15974250461942	25.70745663706265	23.380620293655994
41	19.24869364804991	22.490989648526753	28.87715324551469	29.383163457908644
42	23.641612525083943	24.945262363155905	25.18817428622519	26.22495082553496
43	23.55232361765115	25.12833045240508	26.47155828415887	24.847787645784905
44	20.143490095567344	30.290516778923525	27.692237388488206	21.87375573702092
45	19.178120840038943	36.221016868331645	22.808448073751762	21.79241421787765
46	23.708450060598835	31.41427748306213	25.389839264071846	19.487433192267194
47	22.833124714390735	27.926089288907434	26.36569907214242	22.87508692455942
48	24.61775049174465	24.614651010311736	29.870656255588006	20.896942242355607
49	22.40984681409072	22.62875762452564	31.51501062963184	23.446384931751805
50	21.042299974170987	30.978681130913355	27.284814527825795	20.694204367089867
51	18.751107667242852	33.60457769565476	23.862311497884008	23.782003139218375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3706.0
1	4467.5
2	5229.0
3	15448.5
4	25668.0
5	18515.5
6	11363.0
7	11309.5
8	11256.0
9	12035.0
10	12814.0
11	12809.5
12	12805.0
13	12149.0
14	11493.0
15	11027.0
16	10561.0
17	10470.0
18	10379.0
19	9931.0
20	9483.0
21	9554.5
22	9626.0
23	8890.5
24	8155.0
25	10450.5
26	14874.5
27	17003.0
28	20431.5
29	23860.0
30	25986.0
31	28112.0
32	33357.0
33	38602.0
34	48702.0
35	58802.0
36	60069.5
37	61337.0
38	73530.0
39	85723.0
40	123016.0
41	160309.0
42	202425.0
43	244541.0
44	264426.0
45	284311.0
46	259561.0
47	234811.0
48	217840.0
49	200869.0
50	183914.0
51	166959.0
52	154441.5
53	141924.0
54	133369.5
55	124815.0
56	119356.5
57	113898.0
58	105069.0
59	96240.0
60	89543.0
61	82846.0
62	70562.0
63	58278.0
64	50841.0
65	43404.0
66	38457.5
67	33511.0
68	28333.0
69	23155.0
70	19497.0
71	15839.0
72	12521.5
73	9204.0
74	7518.5
75	4254.0
76	2675.0
77	2365.5
78	2056.0
79	1570.0
80	1084.0
81	836.5
82	589.0
83	472.5
84	356.0
85	254.0
86	152.0
87	111.5
88	71.0
89	55.0
90	39.0
91	30.5
92	22.0
93	16.0
94	10.0
95	10.0
96	10.0
97	12.0
98	14.0
99	8.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2516550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.069624386881504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.32394332908274	13.229148473452787
2	8.820197144573797	2.8347451026309134
3	2.5665500737400553	1.2373048696537714
4	1.1529403393942337	0.7410927277819606
5	0.644043542491262	0.5174768908315568
6	0.42239530092589067	0.4072640297197706
7	0.28722413788933576	0.32309088075092396
8	0.22748791598741497	0.2924516289977771
9	0.17412340098797008	0.2518287885758727
>10	1.3932512752204802	4.770028430267417
>50	0.43085775198656645	5.029878484889379
>100	1.460495828916384	59.81807283750005
>500	0.09177708163863339	8.659324940364268
>1k	0.004216784832045318	1.0020825941492142
>5k	2.4804616659090104E-4	0.24298709204190966
>10k+	2.4804616659090104E-4	0.643222228392437
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16137	0.641235024140192	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6096	0.24223639506467187	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2864	0.11380660030597446	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	2554	0.10148814845721324	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.973694144761678E-5	0.0	0.0	0.04549879795752121	0.0
2	3.973694144761678E-5	0.0	0.0	0.17118674375633308	0.0
3	3.973694144761678E-5	0.0	0.0	0.24187876259164331	0.0
4	3.973694144761678E-5	0.0	0.0	0.4497427033041267	0.0
5	3.973694144761678E-5	0.0	0.0	0.7280602412032345	0.0
6	3.973694144761678E-5	0.0	0.0	1.04043233792295	0.0
7	3.973694144761678E-5	0.0	0.0	1.1706502950467903	0.0
8	3.973694144761678E-5	0.0	0.0	1.4772605352566013	0.0
9	3.973694144761678E-5	0.0	0.0	1.5567344181518348	0.0
10	7.947388289523356E-5	0.0	0.0	1.7236693091732729	0.0
11	7.947388289523356E-5	0.0	0.0	1.9434145953785937	0.0
12	7.947388289523356E-5	0.0	0.0	2.112693965945441	0.0
13	7.947388289523356E-5	0.0	0.0	2.1822336134787705	0.0
14	7.947388289523356E-5	0.0	0.0	2.211122369911188	0.0
15	7.947388289523356E-5	0.0	0.0	2.2598001231845184	0.0
16	7.947388289523356E-5	0.0	0.0	2.368957501341122	0.0
17	7.947388289523356E-5	0.0	0.0	2.4946454471399337	0.0
18	7.947388289523356E-5	0.0	0.0	2.6839919731378274	0.0
19	7.947388289523356E-5	0.0	0.0	2.762551906379766	0.0
20	7.947388289523356E-5	0.0	0.0	2.8545826627724464	0.0
21	7.947388289523356E-5	0.0	0.0	2.97740954878703	0.0
22	7.947388289523356E-5	0.0	0.0	3.094673262998947	0.0
23	7.947388289523356E-5	0.0	0.0	3.225765432834635	0.0
24	7.947388289523356E-5	0.0	0.0	3.319783036299696	0.0
25	7.947388289523356E-5	0.0	0.0	3.403548508871272	0.0
26	7.947388289523356E-5	0.0	0.0	3.48361844588822	0.0
27	7.947388289523356E-5	0.0	0.0	3.5690131330591486	0.0
28	7.947388289523356E-5	0.0	0.0	3.656990721424172	0.0
29	7.947388289523356E-5	0.0	0.0	3.7507699032405477	0.0
30	7.947388289523356E-5	0.0	0.0	3.89012735689734	0.0
31	7.947388289523356E-5	0.0	0.0	4.003536587788838	0.0
32	7.947388289523356E-5	0.0	0.0	4.10303788917367	0.0
33	7.947388289523356E-5	0.0	0.0	4.204963143986808	0.0
34	7.947388289523356E-5	0.0	0.0	4.314041048260515	0.0
35	7.947388289523356E-5	0.0	0.0	4.466392481770678	0.0
36	7.947388289523356E-5	0.0	0.0	4.572847747908844	0.0
37	7.947388289523356E-5	0.0	0.0	4.690230672945104	0.0
38	1.1921082434285033E-4	0.0	0.0	4.811865450716258	0.0
39	1.1921082434285033E-4	0.0	0.0	4.987820627446306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCATA	60	0.0	45.000004	30
CGTAAGA	30	2.1665346E-6	45.000004	10
GTATACG	120	0.0	45.000004	1
CATCGAA	30	2.1665346E-6	45.000004	35
TCGAAGT	30	2.1665346E-6	45.000004	37
TACGTGC	30	2.1665346E-6	45.000004	41
TATCACG	20	7.0348877E-4	45.0	1
AGGTAAC	45	3.8562575E-10	45.0	33
AACCGAT	25	3.8921335E-5	45.0	24
CGAACTA	40	6.8212103E-9	45.0	22
CGTATTA	45	3.8562575E-10	45.0	31
GATCGAC	25	3.8921335E-5	45.0	9
ACGCATA	20	7.0348877E-4	45.0	22
ACGTTAG	80	0.0	45.0	1
ACGTTAC	20	7.0348877E-4	45.0	45
GCGAACT	20	7.0348877E-4	45.0	9
GCGAAAC	40	6.8212103E-9	45.0	23
CACGCGT	20	7.0348877E-4	45.0	38
GCACGAT	35	1.212702E-7	45.0	13
GTCTAGA	25	3.8921335E-5	45.0	37
>>END_MODULE