FastQCFastQC Report
Thu 26 May 2016
SRR1547488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547488_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1252678
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49140.392279580227321No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13680.10920603698636043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGT207.033529E-445.00000421
GGTCGAC207.033529E-445.00000424
CGTTGGA207.033529E-445.00000411
GGCCGTA351.2120472E-745.00000444
TGTTACG750.045.0000041
GCGATCG207.033529E-445.0000049
CGCGTAG207.033529E-445.0000043
TATCGTG406.8157533E-945.0000041
TACGTCG207.033529E-445.00000413
TACGTAA207.033529E-445.00000444
TCTATCG207.033529E-445.00000431
ACGCGTC207.033529E-445.00000428
CGCGTAC302.1656597E-644.99999632
CGTTAGG2650.044.1509442
ACGTTAG900.042.51
CGGGATA3300.042.2727286
ATTAGCG800.042.1875041
TCGATAG750.042.0000041
CGTTTTT27800.041.3579141
TAGCACG603.6379788E-1241.2499961