FastQCFastQC Report
Thu 26 May 2016
SRR1547487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547487_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1286573
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54940.4270259052537244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTACG302.1657052E-645.00000419
GTATTCG207.0336007E-445.01
CGAAACA253.891066E-545.031
ATTCGAC253.891066E-545.035
CAATTCG207.0336007E-445.01
TTAACGA253.891066E-545.022
TTACGTC253.891066E-545.028
TACCGGT351.2120836E-745.013
TATAGCG800.042.18751
TATTACG603.6379788E-1241.2500041
ACGGGAT5300.041.1792455
TAGGGAC10950.040.479455
TATGGGC4900.040.4081654
AACGGGA6150.040.24394
CGTTTTT29250.040.2307741
TACGTAG1350.040.01
TATCCGA451.9286745E-840.044
GGCGATT5600.039.7767838
ATTAACG1200.039.3750041
TCACGAC1600.039.37525
Produced by FastQC (version 0.11.5)